Ncl Synchronously Regulates Na+, K+ and Cl− in Soybean and Greatly Increases the Grain Yield in Saline Field Conditions

Duc, Tuyen; Chen, Huatao; Hiền, Vũ Thị; Hamwieh, Aladdin; Yamada, Tetsuya; Satô, Tadashi; Yan, Yongliang; Huang, Cong; Shono, Masayuki; Suenaga, Kazuhiro; Xu, Donghe

doi:10.1038/srep19147

Cited by 91 publications

(139 citation statements)

References 43 publications

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“…This was similar to the previously reported salt tolerance phenotype of G. max (Tuyen et al, 2016), Brassica napus line N119 (Yong et al, 2014), Oryza sativa line FL478 (Walia et al, 2005), and Foxtail millet cv. Prasad (Puranik et al, 2011), all of which maintained lower Na + concentrations, and ratios of Na + /K + and Na + /Ca 2+ compared with sensitive lines.…”

Section: Discussionsupporting

confidence: 91%

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Identification and Analysis of NaHCO3 Stress Responsive Genes in Wild Soybean (Glycine soja) Roots by RNA-seq

et al. 2016

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Soil alkalinity is a major abiotic constraint to crop productivity and quality. Wild soybean (Glycine soja) is considered to be more stress-tolerant than cultivated soybean (G. max), and has considerable genetic variation for increasing alkalinity tolerance of soybean. In this study, we analyzed the transcriptome profile in the roots of an alkalinity tolerant wild soybean variety N24852 at 12 and 24 h after 90 mM NaHCO3 stress by RNA-sequencing. Compared with the controls, a total of 449 differentially expressed genes (DEGs) were identified, including 95 and 140 up-regulated genes, and 108 and 135 down-regulated genes at 12 and 24 h after NaHCO3 treatment, respectively. Quantitative RT-PCR analysis of 14 DEGs showed a high consistency with their expression profiles by RNA-sequencing. Gene Ontology (GO) terms related to transcription factors and transporters were significantly enriched in the up-regulated genes at 12 and 24 h after NaHCO3 stress, respectively. Nuclear factor Y subunit A transcription factors were enriched at 12 h after NaHCO3 stress, and high percentages of basic helix-loop-helix, ethylene-responsive factor, Trihelix, and zinc finger (C2H2, C3H) transcription factors were found at both 12 and 24 h after NaHCO3 stress. Genes related to ion transporters such as ABC transporter, aluminum activated malate transporter, glutamate receptor, nitrate transporter/proton dependent oligopeptide family, and S-type anion channel were enriched in up-regulated DEGs at 24 h after NaHCO3 treatment, implying their roles in maintaining ion homeostasis in soybean roots under alkalinity. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed “phenylpropanoid biosynthesis” and “phenylalanine metabolism” pathways might participate in soybean response to alkalinity. This study provides a foundation to further investigate the functions of NaHCO3 stress-responsive genes and the molecular basis of soybean tolerance to alkalinity.

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Section: Discussionsupporting

confidence: 91%

“…Maintenance of a low Na + concentration, and low ratios of Na + /K + and Na + /Ca 2+ are widely used as indices of plant salinity tolerance (Puranik et al, 2011; Yong et al, 2014; Tuyen et al, 2016). To evaluate the alkalinity tolerance of wild soybean N24852, the salt tolerant soybean variety Lee 68 was used for comparison.…”

Section: Resultsmentioning

confidence: 99%

Identification and Analysis of NaHCO3 Stress Responsive Genes in Wild Soybean (Glycine soja) Roots by RNA-seq

et al. 2016

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“…Recently, the overexpression of Ncl gene (homologous to the Na + /H + antiporter gene family) into a Japanese soybean salt-sensitive cultivar Kariyutaka, resulted in improved salt tolerance in transgenic soybean. A close association was observed between the high expression of the salt tolerance gene Ncl in the root, the lower accumulation of Na + , K + , and Cl - in the shoot under salt stress and salt tolerance in the transgenic lines (Do et al, 2016). …”

Section: Strategies To Improve Salt Tolerance In Cropsmentioning

confidence: 76%

“…This QTL is likely to be the Ncl locus based on pedigree tracing (Lee et al, 2004). Recently, the introgression of the tolerance allele Ncl into soybean cultivar Jackson, using DNA MAS, produced an improved salt-tolerant line (Do et al, 2016). …”

Section: Strategies To Improve Salt Tolerance In Cropsmentioning

confidence: 99%

New Insights on Plant Salt Tolerance Mechanisms and Their Potential Use for Breeding

et al. 2016

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Soil salinization is a major threat to agriculture in arid and semi-arid regions, where water scarcity and inadequate drainage of irrigated lands severely reduce crop yield. Salt accumulation inhibits plant growth and reduces the ability to uptake water and nutrients, leading to osmotic or water-deficit stress. Salt is also causing injury of the young photosynthetic leaves and acceleration of their senescence, as the Na+ cation is toxic when accumulating in cell cytosol resulting in ionic imbalance and toxicity of transpiring leaves. To cope with salt stress, plants have evolved mainly two types of tolerance mechanisms based on either limiting the entry of salt by the roots, or controlling its concentration and distribution. Understanding the overall control of Na+ accumulation and functional studies of genes involved in transport processes, will provide a new opportunity to improve the salinity tolerance of plants relevant to food security in arid regions. A better understanding of these tolerance mechanisms can be used to breed crops with improved yield performance under salinity stress. Moreover, associations of cultures with nitrogen-fixing bacteria and arbuscular mycorrhizal fungi could serve as an alternative and sustainable strategy to increase crop yields in salt-affected fields.

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“…Haplotype screening of GmSALT3 identified a total of nine haplotypes including two salt-tolerant haplotypes and seven salt-sensitive haplotypes (Guan et al, 2014b). Recently, Do et al (2016) identified the same gene in salt tolerant cultivar FT-Abhayra using map-based cloning and identified same insertion of a ~3.8-kb Ty1/copia type retrotransposon fragment responsible for loss of function of the salt tolerant gene named as Ncl . All of the characterized genes underlying salt tolerant QTLs were pinpointed at single gene Glyma03g32900 , and the presence of Ty1/copia type retrotransposon in all salt tolerant genotypes indicate the single point of origin for salt sensitivity in soybean.…”

Section: Qtlomics: Qtls To Genes In Soybeanmentioning

confidence: 99%

QTLomics in Soybean: A Way Forward for Translational Genomics and Breeding

et al. 2016

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Food legumes play an important role in attaining both food and nutritional security along with sustainable agricultural production for the well-being of humans globally. The various traits of economic importance in legume crops are complex and quantitative in nature, which are governed by quantitative trait loci (QTLs). Mapping of quantitative traits is a tedious and costly process, however, a large number of QTLs has been mapped in soybean for various traits albeit their utilization in breeding programmes is poorly reported. For their effective use in breeding programme it is imperative to narrow down the confidence interval of QTLs, to identify the underlying genes, and most importantly allelic characterization of these genes for identifying superior variants. In the field of functional genomics, especially in the identification and characterization of gene responsible for quantitative traits, soybean is far ahead from other legume crops. The availability of genic information about quantitative traits is more significant because it is easy and effective to identify homologs than identifying shared syntenic regions in other crop species. In soybean, genes underlying QTLs have been identified and functionally characterized for phosphorous efficiency, flowering and maturity, pod dehiscence, hard-seededness, α-Tocopherol content, soybean cyst nematode, sudden death syndrome, and salt tolerance. Candidate genes have also been identified for many other quantitative traits for which functional validation is required. Using the sequence information of identified genes from soybean, comparative genomic analysis of homologs in other legume crops could discover novel structural variants and useful alleles for functional marker development. The functional markers may be very useful for molecular breeding in soybean and harnessing benefit of translational research from soybean to other leguminous crops. Thus, soybean crop can act as a model crop for translational genomics and breeding of quantitative traits in legume crops. In this review, we summarize current status of identification and characterization of genes underlying QTLs for various quantitative traits in soybean and their significance in translational genomics and breeding of other legume crops.

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Ncl Synchronously Regulates Na+, K+ and Cl− in Soybean and Greatly Increases the Grain Yield in Saline Field Conditions

Cited by 91 publications

References 43 publications

Identification and Analysis of NaHCO3 Stress Responsive Genes in Wild Soybean (Glycine soja) Roots by RNA-seq

Identification and Analysis of NaHCO3 Stress Responsive Genes in Wild Soybean (Glycine soja) Roots by RNA-seq

New Insights on Plant Salt Tolerance Mechanisms and Their Potential Use for Breeding

QTLomics in Soybean: A Way Forward for Translational Genomics and Breeding

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