NCL-based mitochondrial-targeting fluorescent probe for the detection of Glutathione in living cells

Visualization of peroxynitrite/GSH cross-talk in the oxidant–antioxidant balance by a dual-fluorophore and dual-site based mito-specific fluorescent probe

“…[13][14][15] However, high-dose ONOO − can cause oxidative stress, indirectly activate the antioxidant program, and increase the content of GSH. [16][17][18][19] As the most commonly found small molecule thiol in most cells, GSH is a scavenger of ONOO − and considered as a cell protection mole-cule that participates in multiple cellular processes, such as differentiation, metabolism, and antioxidant. [20][21][22] The combination of GSH with active oxygen and transformation from reduced GSH to oxidized GSSG play a vital role in the antioxidant process.…”

Early diagnosis of heterotopic ossification with a NIR fluorescent probe by targeting type II collagen

“…As a radiation-free imaging modality with high resolution and sensitivity, fluorescence-based near-infrared (NIR) imaging has been a mature technology in real-time visualization and monitoring of dynamic physiological and pathological processes in living subjects. [12][13][14][15][16][17] However, few data are available concerning the application of NIR imaging in diagnosing HO. Perosky et al reported that NIR imaging with a commercial probe-IRDye 680RD BoneTag revealed dynamic turnover and progression of mineralization in a mouse HO model.…”

Systematic Screening of Trigger Moieties for Designing Formaldehyde Fluorescent Probes and Application in Live Cell Imaging

“…Due to its high sensitivity, selectivity, and spatial and temporal resolution, fluorescent techniques have attracted continuous attention and have been applied in the detection of many types of biomolecules in living organisms [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ]. To date, two primary strategies have been reported for FA fluorescent probe design: one employs the 2−aza−Cope mechanism of rearrangement [ 17 , 18 ], and the other utilizes the condensation reaction mechanism between amines or hydrazones [ 19 , 20 ].…”