NCF4 dependent intracellular reactive oxygen species regulate plasma cell formation

NCF4 regulates antigen presentation of cysteine peptides by intracellular oxidative response and restricts activation of autoreactive and arthritogenic T cells

“…The Ncf1 m1J mutation leads to expression of only small amounts of deficient NCF1 protein, abrogating both intracellular and extracellular ROS response by the NOX2 complex [ 11 , 30 ]. We also showed that mice with the NCF4 58A mutation developed severe CIA due to dysregulated B cells and lost the ability to regulate plasma cell differentiation [ 31 ]. These data suggested that NCF4 and NCF1 regulate autoimmunity and the development of arthritis differentially.…”

“…To induce GIA in mice, all the male mice over 3 months of age were given a single intradermal injection of 100 μL of emulsified hGPI 325-339 peptide (10 μg per mouse) 1:1 complete Freund's adjuvant (CFA) (10 mg/mL Mycobacterium tuberculosis H37Ra (BD 231141) in incomplete Freund's adjuvant (IFA) (BD 263910)) as described previously [ 54 ]. To induce CIA in mice, all the mice over 3 months were given a single intradermal injection of 100 μL of emulsified type II collagen (50 μg per mouse) 1:1 CFA (BD, 263810) as described previously [ 31 ]. Arthritis severity was scored daily after immunization, as earlier described in detail [ 55 ].…”

Gene expression analysis of anti-TNF-treated rheumatoid arthritis patients reveals the links between NADPH oxidase expression profile and immunophenotypic changes in peripheral blood mononuclear cells

“…Only recently, in vivo study on animal model showed that the deletion of NCF4, through impaired intracellular ROS production in activated B cells, led to increased differentiation of B cells into plasma cells as well as decreased expression of C-X-C Motif Chemokine Receptor 4 (CXCR4, CD184) and increased C-X-C Motif Chemokine Receptor 3 (CXCR3, CD183) [32]. CXCR3 and CXCR4 are chemokine receptors, highly expressed in RA patients.…”