NCAPH plays important roles in human colon cancer

Abstract

Colon cancer (CC) is one of the major malignancies worldwide, whose pathogenesis is complex and requires the accumulated alteration of multiple genes and signaling pathways. Condensins are multi-protein complexes that play pivotal roles in chromosome assembly and segregation during mitosis, meiosis and even tumorigenesis. Using tissue microarrays by immunohistochemistry and hematoxylin–eosin staining, we found that non-SMC condensin I complex subunit H (NCAPH) in colon cancerous tissues was higher than that in all corresponding adjacent non-cancerous tissues. We then characterized the exact function of the NCAPH in CC. We provided evidences showing that NCAPH is highly expressed in colorectal cancer cell lines comparing with normal human colonic epithelial cells, and identified many NCAPH mutations in CC patients. We found that depletion of NCAPH inhibits CC cell proliferation, migration in vitro and xenograft tumor formation in vivo. Furthermore, NCAPH knockdown promotes cell apoptosis and cell cycle arrest at G2/M phase. Interestingly, the NCAPH high expression in tumor tissues of colon patients had a significantly better prognosis and survival rate than low-expression patients, suggesting that NCAPH high expression promotes colonic cancerous cell proliferation; on the other hand, it may also sensitize these cells responding to chemo- or radio-therapies. Collectively, these findings reveal an important role of NCAPH in CC, indicating that NCAPH could be used as a new therapeutic target in future.

Micro‐ribonucleic acid expression signature of metastatic castration‐resistant prostate cancer: Regulation of NCAPH by antitumor miR‐199a/b‐3p

“…A recent report showed that NCAPH is upregulated in colon cancer tissue, and its high expression correlates with poor prognosis. Furthermore, inhibiting NCAPH promotes cell apoptosis and cell cycle arrest at the G2/M phase, and exerts antitumor effects in colorectal cancer in vitro and in vivo . Importantly, our present data showed that regardless of the expression of AR, the silencing of NCAPH inhibited the aggressiveness of PCa cells.…”

“…Furthermore, inhibiting NCAPH promotes cell apoptosis and cell cycle arrest at the G2/M phase, and exerts antitumor effects in colorectal cancer in vitro and in vivo. 33 Importantly, our present data showed that regardless of the expression of AR, the silencing of NCAPH inhibited the aggressiveness of PCa cells. This is the first study to report that dysregulated NCAPH is deeply involved in CRPC pathogenesis, and its expression was directly controlled by antitumor miR-199a/b-3p.…”

Identification and Characterization of a New Platinum-Induced TP53 Mutation in MDAH Ovarian Cancer Cells

“…C21orf45, also known as MIS18A, is a kinetocore protein belonging to the Mis18 complex assembly that is crucial for CENPA deposition at the centromere [26,27]. DEPDC1 is highly expressed in M phase and its silencing caused mitotic defects [28][29][30][31] and NCAPH is a regulatory subunit of the condensin complex required for the conversion of interphase chromatin into condensed chromosomes [32][33][34]. Overall, their altered expression could participate in the definition of the aberrant mitotic regulation.…”

Non‐SMC condensin I complex subunit H enhances proliferation, migration, and invasion of hepatocellular carcinoma

“…NCAPH encodes a regulatory subunit in the non‐SMC condensing I complex and is required for the conversion of interphase chromatin into condensed chromosomes . Previous studies have revealed that abnormal expression of NCAPH is associated with colon cancer and prostate cancer . However, there have been no relevant reports about the clinical relevance and functional role of NCAPH in HCC.…”

“…9 Previous studies have revealed that abnormal expression of NCAPH is associated with colon cancer and prostate cancer. 10,11 However, there have been no relevant reports about the clinical relevance and functional role of NCAPH in HCC. In this study, we aimed to explore NCAPH expression in HCC tissues and investigate the functions of NCAPH in HCC cells.…”