NCAPG upregulation mediated by four microRNAs combined with activation of the p53 signaling pathway is a predictor of poor prognosis in patients with breast cancer

Dong, Menglu; Cui, Xiaoqing; Li, Hanning; Li, Xingrui; Xia, Wenfei

doi:10.3892/ol.2021.12585

Cited by 16 publications

(16 citation statements)

References 39 publications

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“…First, we comprehensively analyzed the TCGA-glioma and GEO (GSE 10611 and 4290) datasets and detected a high expression of NCAPG in glioma; we then verified the results using the GEPIA online website. The functional enrichment of differentially expressed genes and NCAPG in gliomas predicted that NCAPG may be closely related to the cell cycle ( 32 ), p53 signaling pathway ( 33 ), and PI3K/ARK signaling pathway ( 34 ) in glioma, which is consistent with previous studies on NCAPG in tumors. Furthermore, we analyzed the relationship between the expression of NCAPG in glioma and the survival time of glioma patients, tumor classification, and IDH mutation using TCGA data.…”

Section: Discussionsupporting

confidence: 90%

NCAPG Promotes Tumor Progression and Modulates Immune Cell Infiltration in Glioma

Zheng

Han

et al. 2022

Front. Oncol.

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Glioma is one of the most deadly types of brain cancer. As it is highly invasive, the prognosis for glioma patients remains dismal, with median survival rarely exceeding 16 months. Thus, developing a new prognostic biomarker for glioma and investigating its molecular mechanisms is necessary for the development of an efficient treatment strategy. In this study, we analyzed a cohort of 1,131 glioma patients using RNA-seq data from The Cancer Genome Atlas (TCGA project) and Gene Expression Omnibus (GSE4290 and GSE16011 datasets), and validated the results using the RNA-seq data of 1,018 gliomas from the Chinese Glioma Genome Atlas (CGGA project). We used the R language as the main tool for statistical analysis and data visualization. We found that NCAPG, a mitosis-associated chromosomal condensing protein, is highly expressed in glioma tissues. Furthermore, the expression of NCAPG increased significantly with the increase in tumor grade, and high NCAPG expression was found to be a predictor of poor overall survival in glioma patients (P < 0.001). This result shows that NCAPG expression could be an independent prognostic factor. Importantly, when the expression of NCAPG was knocked down, the CCK-8 assay revealed that the proliferation of glioma cells (LN-229 and T98G cell lines) decreased significantly compared with the control group. In addition, the healing rates of these cells were significantly lower in the si-NCAPG group than in the control group (P < 0.001). We then used the CIBERSORT algorithm to analyze the expression levels of 22 subpopulations of immune cells and found that NCAPG was significantly negatively correlated with natural killer cell activation. In addition, it was positively correlated with MHC-I molecules and ADAM17. Our study is first in comprehensively describing the high expression of NCAPG in glioma. It also shows that NCAPG can function as an independent prognostic predictor of glioma, and that targeting NCAPG can be a new strategy for the treatment of glioma patients.

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Section: Discussionsupporting

confidence: 90%

NCAPG Promotes Tumor Progression and Modulates Immune Cell Infiltration in Glioma

Zheng

Han

et al. 2022

Front. Oncol.

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“…Knockdown of NCAPG has been shown to regulate tumor proliferation, cell cycle and apoptosis through multiple signaling pathways [ 34 – 36 ]. Zhang et al [ 11 ] demonstrated that NCAPG regulates cardia adenocarcinoma progression both in vivo and in vitro via the PI3K/AKT signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

NCAPG facilitates colorectal cancer cell proliferation, migration, invasion and epithelial–mesenchymal transition by activating the Wnt/β-catenin signaling pathway

Shi

Fang

et al. 2022

Cancer Cell Int

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Background The condensation complex gene non-SMC condensin I complex subunit G(NCAPG), a cell cycle-associated condensin, is over-expressed in various cancers. However, its biological function in colorectal cancer (CRC) has yet to be deciphered. In this study, we investigated the role of NCAPG in CRC progression. Methods Tissues and cells were used to measure NCAPG expression levels and their association with clinicopathological characteristics. NCAPG silencing and overexpression in CRC cells were used to measure its effect on proliferation, migration, invasion, and epithelial–mesenchymal transition (EMT) progression. In addition, mRNA, and protein expression levels of key EMT biomarkers were measured. The underlying mechanism of NCAPG modulating CRC progression was further explored using western blotting, co-immunoprecipitation (CO-IP), and immunofluorescence (IF) assays. Results NCAPG was over-expressed in CRC tissues and cell lines. High expression levels were associated with differentiation levels, lymph metastasis, and vascular invasion in patients. NCAPG silencing suppressed, while NCAPG overexpression promoted the proliferative, migration, and invasive capacity of HCT116 and SW480 cells. Mechanistically, we discovered that NCAPG participated in regulating the EMT process and the Wnt/β-catenin signaling pathway to facilitate CRC invasion and metastasis. Additional experiments demonstrated that NCAPG activated the Wnt/β-catenin signaling pathway by binding to β-catenin in CRC cells. Conclusion NCAPG acts as an oncogene involved in the development and progression of CRC by binding to β-catenin to activate the Wnt/β-catenin signaling pathway.

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“…Non-SMC condensing complex subunit G (NCAPG) is a crucial component of the condensing complex, which binds to chromosomes at start of mitotic division and dissociates from them when mitotic division ends. Previous studies have reported that NCAPG is a promising therapeutic target across different tumor types, and it may be associated with development and progression of hepatocellular carcinoma (HCC) [25,26], esophageal squamous cell carcinoma (ESCC) [27], colorectal cancer (CRC) [28], breast cancer (BC) [29], prostate cancer (PCa) [30], kidney renal papillary cell carcinoma (KIRP) [31], endometrial cancer (EC) [32], bladder cancer (BLCA) [33], gastric cancer (GC) [34], glioblastoma (GBM) [35], and so on. And in the present study, we also find NCAPG was highly associated with the prognosis of ES; therefore, NCAPG may be a potential therapeutic target.…”

Section: Discussionmentioning

confidence: 99%

Construction of a Prognosis-Related Gene Signature by Weighted Gene Coexpression Network Analysis in Ewing Sarcoma

Zhao

Xiong

Zhang

et al. 2022

Computational and Mathematical Methods in Medicine

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Background. Ewing sarcoma (ES) is the second most common pediatric bone tumor with a high rate of metastasis, high recurrence, and low survival rate. Therefore, the identification of new biomarkers which can improve the prognosis of ES patients is urgently needed. Methods. Here, GSE17679 dataset was downloaded from GEO databases. WGCNA method was used to identify one module associating with OVS (overall vital survival) and event. cytoHubba was used to screen out 50 hub genes from the module genes. Then, GSE17679 dataset was randomly divided into train cohort and test cohort. Next, univariate Cox analysis, LASSO regression analysis, and multivariate Cox analysis were conducted on 50 hub genes combined with train cohort data to select pivotal genes. Finally, an optimal 7-gene-based risk assessment model was established, which was verified by test cohort, entire GSE17679, and two independent datasets (GSE63157 and TCGA-SARC). Results. The results of the functional enrichment analysis revealed that the OVS and event-associated module were mainly enriched in the protein transcription, cell proliferation, and cell-cycle control. And the train cohort was divided into high-risk and low-risk subgroups based on the median risk score; the results showed that the survival of the low-risk subgroup was significantly longer than high-risk. ROC analysis revealed that AUC values of 1, 3, and 5-year survival were 0.85, 0.94, and 0.88, and Kaplan-Meier analysis also revealed that P value < 0.0001, indicating that this model was accurate, which was also verified in the test, entire cohort, and two independent datasets (GSE63157 and TCGA-SARC). Then, we performed a comprehensive analysis (differential expression analysis, correlation analysis and survival analysis) of seven pivotal genes, and found that four genes (NCAPG, KIF4A, NUF2 and CDC20) plays a more crucial role in the prognosis of ES. Conclusion. Taken together, this study established an optimal 7-gene-based risk assessment model and identified 4 potential therapeutic targets, to improve the prognosis of ES patients.

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NCAPG upregulation mediated by four microRNAs combined with activation of the p53 signaling pathway is a predictor of poor prognosis in patients with breast cancer

Cited by 16 publications

References 39 publications

NCAPG Promotes Tumor Progression and Modulates Immune Cell Infiltration in Glioma

NCAPG Promotes Tumor Progression and Modulates Immune Cell Infiltration in Glioma

NCAPG facilitates colorectal cancer cell proliferation, migration, invasion and epithelial–mesenchymal transition by activating the Wnt/β-catenin signaling pathway

Construction of a Prognosis-Related Gene Signature by Weighted Gene Coexpression Network Analysis in Ewing Sarcoma

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