NB-LRR genes: characteristics in three Solanum species and transcriptional response to Ralstonia solanacearum in tomato

Shi, Jianlei; Zai, Wen Shan; Xiong, Zhi Li; Wan, Hong; Wu, Wei

doi:10.1007/s00425-021-03745-7

Cited by 4 publications

(7 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The distribution of NLR genes on tomato chromosomes observed in the present study was consistent with that reported by Shi et al (2021 ). The maximum number of genes was distributed on chromosomes 4, 5, and 11.…”

Section: Discussionsupporting

confidence: 93%

“…The difference in the number of NLR genes between present research and earlier studies within tomato is due to improved genome annotation, selective strategies, and threshold differences for NLR gene identification ( Seo et al, 2016 ; Arafa et al, 2017 ; Shi et al, 2021 ). Genome size does not correlate with the number of NLR genes present in various plants, as potato has a smaller genome than tomato but more NLRs due to evolution ( Li et al, 2021 ).…”

Section: Discussionmentioning

confidence: 64%

“…A small proportion of NLR proteins show N-terminus resistance to powdery mildew 8 (RPW8) domains (RNL) ( Shao et al, 2014 ). Another variable region, the LRR domain at the C-terminus, makes the full-length gene sequences TNL, CNL, and RNL, while some genes lack and encode the partial domain sub-families CN, TN, and N. Both variable NLR regions exhibit specificity toward pathogen recognition by interacting with specific ligands to introduce resistance and are implicated in the activation of corresponding pathways ( Shi et al, 2021 ). Moreover, NLR required for cell death (NRC) works as a helper to NLRs, which evolve via duplication and establish specificities to diverse pathogens together with sensor NLRs ( Wu et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genome-wide characterization of the NLR gene family in tomato (Solanum lycopersicum) and their relatedness to disease resistance

Bashir

Rehman²,

Zaman³

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

Nucleotide-binding leucine-rich-repeat receptors (NLR), the largest group of genes associated with plant disease resistance (R), have attracted attention due to their crucial role in protecting plants from pathogens. Genome-wide studies of NLRs have revealed conserved domains in the annotated tomato genome. The 321 NLR genes identified in the tomato genome have been randomly mapped to 12 chromosomes. Phylogenetic analysis and classification of NLRs have revealed that 211 genes share full-length domains categorized into three major clades (CNL, TNL, and RNL); the remaining 110 NLRs share partial domains and are classified in CN, TN, and N according to their motifs and gene structures. The cis-regulatory elements of NLRs exhibit the maximum number of these elements and are involved in response to biotic and abiotic stresses, pathogen recognition, and resistance. Analysis of the phylogenetic relationship between tomato NLRs and orthologs in other species has shown conservation among Solanaceae members and variation with A. thaliana. Synteny and Ka/Ks analyses of Solanum lycopersicum and Solanum tuberosum orthologs have underscored the importance of NLR conservation and diversification from ancestral species millions of years ago. RNA-seq data and qPCR analysis of early and late blight diseases in tomatoes revealed consistent NLR expression patterns, including upregulation in infected compared to control plants (with some exceptions), suggesting the role of NLRs as key regulators in early blight resistance. Moreover, the expression levels of NLRs associated with late blight resistance (Solyc04g007060 [NRC4] and Solyc10g008240 [RIB12]) suggested that they regulate S. lycopersicum resistance to P. infestans. These findings provide important fundamental knowledge for understanding NLR evolution and diversity and will empower the broader characterization of disease resistance genes for pyramiding through speed cloning to develop disease-tolerant varieties.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-wide characterization of the NLR gene family in tomato (Solanum lycopersicum) and their relatedness to disease resistance

Bashir

Rehman²,

Zaman³

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

show abstract

“…One of the most studied and characterized plant defense mechanisms is mediated by resistance genes ( R ). Most R genes code for a protein with a nucleotide binding site and a leucine-rich repeat domain (NBS-LRR) (Shi et al 2021 ). When NBS-LRR-containing proteins recognize avirulence factors encoded by pathogen Avr genes, programmed cell death is induced as part of a hypersensitive response (HR) (Jubic et al 2019 ; Mur et al 2008 ).…”

Section: Dominant Resistance (R) and Recessive Gene Repertoire Agains...mentioning

confidence: 99%

Understanding tobamovirus-plant interactions: implications for breeding resistance to tomato brown rugose fruit virus

et al. 2022

View full text Add to dashboard Cite

The genus Tobamovirus comprises a group of single-stranded RNA viruses that affect a wide variety of vegetables of economic importance. Tobamoviruses express a series of proteins that interact with the plant’s cellular machinery, allowing viral infection; during incompatible interactions, active defense is mediated by host proteins encoded by resistance genes. The genes conferring viral resistance and tolerance in non-susceptible hosts have been studied for their ability to transfer desired resistance traits to different crops. The N gene from Nicotiana spp., the repertoire of Tm genes in Solanum spp., the L locus from Capsicum spp., and TOM genes are the most studied genetic sequences for understanding resistance to tobamoviruses. Through classical plant breeding and genetic engineering techniques, it has been possible to introgress these resistance genes ( R ) into new species. However, new reports highlight the ability of tobamoviruses to overcome R -mediated defense. One of the most notorious recent cases is the tomato brown rugose fruit virus (ToBRFV). The main characteristic of ToBRFV is its capacity to overcome the resistance mediated by the Tm-2 2 gene, resulting in a limited repertoire of options to combat the virus. To defeat emerging viruses, it is necessary to apply the knowledge from other tobamoviruses-host relationships and use new technologies such as genome-wide association studies (GWAS) to understand and associate the architecture of resistance genes present in the Solanaceae family for the benefit of plant breeding. Although new genomic tools such as CRISPR systems open the possibility of coping with viral diseases, there are no commercial ToBRFV-resistant tomato varieties. Hence, the world’s leading seed suppliers compete to develop and bring these varieties to market. Supplementary Information The online version contains supplementary material available at 10.1007/s42161-022-01287-9.

show abstract

“…This TF is involved in defense responses, and a binding motif found at -1065 nt from the start codon of the Tsw gene. Other MYB TFs have also been shown to be involved in defense responses (Liu et al, 2004;Shi et al, 2021;Zhu et al, 2022)…”

mentioning

confidence: 99%

Mind the gap : Analysis of Tsw resistance against tospoviruses

Grinsven¹

View full text Add to dashboard Cite

Tospoviruses are among the most important plant pathogens and cause serious crop losses worldwide. Tospoviruses have evolved to smartly utilize the host cellular machinery to accomplish their life cycle. Plants mount two layers of defense to combat their invasion. The first one involves the activation of an antiviral RNA interference (RNAi) defense response. However, tospoviruses encode an RNA silencing suppressor that enables them to counteract antiviral RNAi. To further combat viral invasion, plants also employ intracellular innate immune receptors (e.g., Sw-5b and Tsw) to recognize different viral effectors (e.g., NSm and NSs). This leads to the triggering of a much more robust defense against tospoviruses called effector-triggered immunity (ETI). Tospoviruses have further evolved their effectors and can break Sw-5b-/Tsw-mediated resistance. The arms race between tospoviruses and both layers of innate immunity drives the coevolution of host defense and viral genes involved in counter defense. In this review, a state-of-the-art overview is presented on the tospoviral life cycle and the multilined interplays between tospoviruses and the distinct layers of defense.

show abstract

NB-LRR genes: characteristics in three Solanum species and transcriptional response to Ralstonia solanacearum in tomato

Cited by 4 publications

References 52 publications

Genome-wide characterization of the NLR gene family in tomato (Solanum lycopersicum) and their relatedness to disease resistance

Genome-wide characterization of the NLR gene family in tomato (Solanum lycopersicum) and their relatedness to disease resistance

Understanding tobamovirus-plant interactions: implications for breeding resistance to tomato brown rugose fruit virus

Mind the gap : Analysis of Tsw resistance against tospoviruses

Contact Info

Product

Resources

About