NAuRA: Genomic Tool to Identify Staphylococcal Enterotoxins in Staphylococcus aureus Strains Responsible for FoodBorne Outbreaks

Merda, Déborah; Felten, Arnaud; Vingadassalon, Noémie; Denayer, Sarah; Titouche, Yacine; Decastelli, Lucia; Hickey, Bernadette B; Kourtis, Christos; Daskalov, H.; Mistou, Michel‐Yves; Hennekinne, Jacques‐Antoine

doi:10.3389/fmicb.2020.01483

Cited by 26 publications

(55 citation statements)

References 55 publications

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“…The results of the present European study were not in agreement with the results published by Omoe et al ( 40 ), who detected SEI in only 40% of the strains and SEG was not detected at all. In our study, SEG was produced by 96% of the strains and SEI for 100% of the strains, being positive for the two enterotoxin genes detected by NAuRa ( 35 ). Only for one strain (18SBCL678) was seg predicted, but SEG enterotoxin was not detected.…”

Section: Discussionmentioning

confidence: 57%

“…For the present study, 75 strains were chosen, originating from both human hosts and animal (cattle) as well as from environmental and food sources, with special attention on SFPO strains ( 35 ). Out of the 75 strains, 60 were allocated to the 15 previously defined v Saβ types ( 30 ).…”

Section: Discussionmentioning

confidence: 99%

“…If WGS data are not available, the standard MLST procedure can be performed using standard PCR and Sanger sequencing for the seven housekeeping genes ( arcC , aroE , glpF , gmk , pta , tpiA , and yqiL ) ( 42 ). Instead of the original primers ( 35 ), the newly designed primer by Boss et al ( 43 ) can be applied. They enable unidirectional Sanger sequencing, which considerably saves cost, work, and time.…”

Section: Discussionmentioning

confidence: 99%

“…to SFPO strains (35). Out of the 75 strains, 60 were allocated to the 15 previously defined vSaβ types (30).…”

Section: Downloaded Frommentioning

confidence: 99%

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Staphylococcal Enterotoxin Gene Cluster: Prediction of Enterotoxin (SEG and SEI) Production and of the Source of Food Poisoning on the Basis of v Saβ Typing

Schwendimann

Merda

Berger

et al. 2021

Appl Environ Microbiol

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Currently only five (SEA-SEE) out of 27 known staphylococcal enterotoxins can be analyzed using commercially available kits.Six genes (seg, sei, sem, sen, seo, and seu), encoding putative and undetectable enterotoxins, are located on the enterotoxin gene cluster (egc) which is part of the Staphylococcus aureus genomic island vSaβ. These enterotoxins have been described as likely being involved in staphylococcal food poisoning outbreaks.The aim of the present study was to determine if whole genome data can be used for the prediction of staphylococcal egc enterotoxin production, particularly enterotoxin G (SEG) and enterotoxin I (SEI). For this purpose whole genome sequences of 75 Staphylococcus aureus (S. aureus) strains from different origins (food poisoning outbreaks, human, and animal) were investigated applying bioinformatics methods (phylogenetic analysis using the core genome and different alignments). SEG and SEI expression was tested in vitro using a sandwich ELISA method.Strains could be allocated to 14 different vSaβ types, each type being associated with a single clonal complex (CC). In addition the vSaβ type and CC were associated with the origin of the strain (human or cattle derived). The amount of SEG and SEI produced also correlated with the vSaβ type and the CC of a strain. The present results show promising indications that the in vitro production of SEG and SEI can be predicted based on the vSaβ type or CC of a strain.IMPORTANCE Beside the infection properties in human and animals, S. aureus can produce different enterotoxins in food. The enterotoxins can cause vomiting and diarrhea, often involving many people. Most of these outbreaks remain undiscovered as detection methods for enterotoxins are only available for a few enterotoxins, but not for the more recently discovered enterotoxin G (SEG) and I (SEI).In this study we show promising results that in vitro production of SEG and SEI can be predicted based on the whole genome sequencing data of a strain. In addition, this data could be used to find the source (human- or cattle-derived) of an outbreak strain, which is the key for a better understanding of the role SEG and SEI play in foodborne outbreaks caused by S. aureus.

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Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…to SFPO strains (35). Out of the 75 strains, 60 were allocated to the 15 previously defined vSaβ types (30).…”

Section: Downloaded Frommentioning

confidence: 99%

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Staphylococcal Enterotoxin Gene Cluster: Prediction of Enterotoxin (SEG and SEI) Production and of the Source of Food Poisoning on the Basis of v Saβ Typing

Schwendimann

Merda

Berger

et al. 2021

Appl Environ Microbiol

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“…The resulting assembly was annotated using PROKKA v1.14.6 ( 18 ), and downstream analyses were performed using SCC mec Finder, SPAtyper v0.1.0, and TA finder ( 19 – 21 ). The NAuRA-curated enterotoxin database ( 22 ) was used to predict toxin gene clusters. ResFinder and PathogenFinder were used to identify the antibiotic resistance and virulence gene clusters, respectively ( 23 , 24 ).…”

Section: Announcementmentioning

confidence: 99%

Draft Genome Sequence of the Community-Associated Staphylococcus aureus Sequence Type 88 Strain LVP-7, Isolated from an Ocular Infection

Nadig

Murthy

Vandanashree

et al. 2021

Microbiol Resour Announc

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We report a de novo-assembled draft genome sequence of the Indian Staphylococcus aureus sequence type 88 (ST88) strain LVP-7, isolated from an ocular infection. The genome harbors a Panton-Valentine leukocidin phage, a type V staphylococcal cassette chromosome mec element, the delta-hemolysin-converting Newman phage ΦNM3, and the pathogenicity island SaPI3, encoding the superantigen enterotoxin B.

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Clonal Diversity, Antimicrobial Susceptibility and Presence of Genes Encoding Virulence Factors in Staphylococcus aureus Strains Isolated from Cut Wound Infections

et al. 2022

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NAuRA: Genomic Tool to Identify Staphylococcal Enterotoxins in Staphylococcus aureus Strains Responsible for FoodBorne Outbreaks

Cited by 26 publications

References 55 publications

Staphylococcal Enterotoxin Gene Cluster: Prediction of Enterotoxin (SEG and SEI) Production and of the Source of Food Poisoning on the Basis of v Saβ Typing

Staphylococcal Enterotoxin Gene Cluster: Prediction of Enterotoxin (SEG and SEI) Production and of the Source of Food Poisoning on the Basis of v Saβ Typing

Draft Genome Sequence of the Community-Associated Staphylococcus aureus Sequence Type 88 Strain LVP-7, Isolated from an Ocular Infection

Clonal Diversity, Antimicrobial Susceptibility and Presence of Genes Encoding Virulence Factors in Staphylococcus aureus Strains Isolated from Cut Wound Infections

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