Nature of the T Lymphocytes in Lymphocyte Predominance Hodgkin's Disease

Dargent, Jean‐Louis; Schandené, Liliane; Kornreich, Anne; Lespagnard, Laurence; Cochaux, Pascale; Jagodzinski, Robert; Capel, Paul D.; Velu, Thierry; Nève, Pierre

doi:10.3109/10428199709055592

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Another remarkable finding was detection of high levels of IFN γ transcripts in the three different NLPHL T cell subsets compared to the tonsillar T cell subsets. Dargent et al 10 previously analysed the cytokine profile of CD4 + T cells from one case of diffuse NLPHL and reported secretion of IL2 and IFNγ together with lack of secretion of IL4 and IL5 by ELISA. Although the CD4 + T cells in their study were composed of a heterogeneous group of T cells, these results are generally consistent with our findings in NLPHL.…”

Section: Discussionmentioning

confidence: 99%

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Cytokine gene expression profile distinguishes CD4⁺/CD57⁺ T cells of the nodular lymphocyte predominance type of Hodgkin's lymphoma from their tonsillar counterparts

Atayar

Poppema

Visser

et al. 2005

The Journal of Pathology

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Little is known about the cytokine profile of nodular lymphocyte predominance Hodgkin's lymphoma (NLPHL) and the significance of the characteristic rosetting CD4(+)/CD57(+) T cells. We analysed the T lymphocyte populations isolated from lymph node suspensions from five patients with NLPHL, two with follicular hyperplasia and progressive transformation of germinal centres (PTGC), three with classical Hodgkin's lymphoma (CHL) and five with hyperplasia of the tonsil. We sorted the T cells based on expression of CD3, CD4 and CD57 by flow cytometry and evaluated the cytokine mRNA profiles of the T cells with quantitative RT-PCR. NLPHL cases were as rich in T cells as the CHL cases, but all NLPHL cases had a much higher frequency of CD4(+)/CD57(+) T cells. In contrast to the CD4(+)/CD57(+) T cells from tonsils, IL2 and IL4 mRNAs were consistently absent from the CD4(+)/CD57(+) T cells of NLPHL. Even after stimulation, no IL4 transcripts could be detected in the CD4(+)/CD57(+) T cells of NLPHL. On the other hand, IFNgamma transcripts were elevated in NLPHL and PTGC T cell subsets as compared to tonsillar T cell subsets. IL13 mRNA was exclusively produced by the T cells of CHL cases, indicating that IL13 may be a key cytokine in CHL. In conclusion, elevated levels of CD4(+)/CD57(+) T cells are characteristic of NLPHL and these T cells display a distinct cytokine mRNA profile.

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Section: Discussionmentioning

confidence: 99%

“…CD4 + /CD57 + T cells of NLPHL are not only present in rosettes around the L&H cells but are also distributed throughout the nodules of NLPHL. Little is known about the cytokine profile of the T cells in NLPHL 9, 10.…”

Section: Introductionmentioning

confidence: 99%

Cytokine gene expression profile distinguishes CD4⁺/CD57⁺ T cells of the nodular lymphocyte predominance type of Hodgkin's lymphoma from their tonsillar counterparts

Atayar

Poppema

Visser

et al. 2005

The Journal of Pathology

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Langerhans' cell histiocytosis associated with simultaneous lymphocyte predominance Hodgkin's disease and malignant melanoma

et al. 1998

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Lymphocyte Predominance Hodgkin's Disease

Kraus

Haley

2000

The American Journal of Surgical Pathology

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Distinction of lymphocyte predominance Hodgkin's disease (LPHD) from other forms of lymphoma often requires immunohistochemistry (IHC). Most previously published recommended panels include markers to define the large neoplastic cells (for example, CD20, J chain, CD45) as well as the non-neoplastic background cells (CD21, CD45RO, CD57, TiA 1). In the present study we examine the practical use of a double IHC method designed to look simultaneously at two germinal center specific cell types: bcl6+ cells and [bc16+, CD57+] co-positive cells. All 10 nodular LPHD had bcl6+ large cells and numerous CD57+ small background cells, including [bcl6+CD57+] cells in rosettes. One case of LPHD with large cell transformation contained numerous bcl6+ large cells both singly (in areas of typical LPHD) and in sheets (in foci of large cell transformation), many CD57+ small cells but few [bcl6+CD57+] co-positive cells and no rosettes. In none of the five cases of florid progressive transformation of germinal centers were true rosettes seen, although all contained variable numbers of bcl6+ large cells and CD57+ cells. Lymphocyte-rich classic Hodgkin's disease LRCHD cases were notable for bcl6 reactivity in Reed-Sternberg cells in all cases, numerous background small bcl6+ lymphocytes, and rare CD57+ cells. Two phenotypic profiles were associated with the 10 cases of T cell-rich B cell large cell lymphoma (TCRBCL). In the first, group "A," six of six cases had bc16- large cells and few CD57+ small cells, and none had significant numbers of [bcl6+, CD57+] co-positive cells. In the second, group "B," four of four cases had bcl6+ large cells with numerous CD57+ and [bcl6+, CD57+] co-positive cells. These findings not only show that LPHD can be distinguished from its morphologic mimics through identification of specific germinal center cell types, but also identifies a second group of TCRBCL (group "B") whose phenotype suggests it might be an architectural variant of nodular LPHD.

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Nature of the T Lymphocytes in Lymphocyte Predominance Hodgkin's Disease

Cited by 3 publications

References 20 publications

Cytokine gene expression profile distinguishes CD4⁺/CD57⁺ T cells of the nodular lymphocyte predominance type of Hodgkin's lymphoma from their tonsillar counterparts

Cytokine gene expression profile distinguishes CD4⁺/CD57⁺ T cells of the nodular lymphocyte predominance type of Hodgkin's lymphoma from their tonsillar counterparts

Langerhans' cell histiocytosis associated with simultaneous lymphocyte predominance Hodgkin's disease and malignant melanoma

Lymphocyte Predominance Hodgkin's Disease

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Nature of the T Lymphocytes in Lymphocyte Predominance Hodgkin's Disease

Cited by 3 publications

References 20 publications

Cytokine gene expression profile distinguishes CD4+/CD57+ T cells of the nodular lymphocyte predominance type of Hodgkin's lymphoma from their tonsillar counterparts

Cytokine gene expression profile distinguishes CD4+/CD57+ T cells of the nodular lymphocyte predominance type of Hodgkin's lymphoma from their tonsillar counterparts

Langerhans' cell histiocytosis associated with simultaneous lymphocyte predominance Hodgkin's disease and malignant melanoma

Lymphocyte Predominance Hodgkin's Disease

Contact Info

Product

Resources

About

Cytokine gene expression profile distinguishes CD4⁺/CD57⁺ T cells of the nodular lymphocyte predominance type of Hodgkin's lymphoma from their tonsillar counterparts

Cytokine gene expression profile distinguishes CD4⁺/CD57⁺ T cells of the nodular lymphocyte predominance type of Hodgkin's lymphoma from their tonsillar counterparts