Nature of Myocardial Contraction and of Action Potentials. Importance of the »Cationlc Gradient»

Lenzi, F; Caniggia, A

doi:10.1111/j.0954-6820.1953.tb10244.x

Cited by 20 publications

(3 citation statements)

References 19 publications

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“…Cardiomyocytes contract via a mechanism known as "excitation-contraction coupling". This process is initiated by an action potential that depolarizes the plasma membrane of cardiomyocytes, which triggers the opening of L-type calcium channels (LTCCs) that allow the influx of extracellular calcium (Figure 1) [10]. Calcium is also released from intracellular stores in the sarcoplasmic reticulum (SR) through ryanodine receptors (RyR2, in fact) in a process known as "calcium-induced calcium release" [10].…”

Section: Introductionmentioning

confidence: 99%

“…Cells 2023, 12, x FOR PEER REVIEW 2 of 16 influx of extracellular calcium (Figure 1) [10]. Calcium is also released from intracellular stores in the sarcoplasmic reticulum (SR) through ryanodine receptors (RyR2, in fact) in a process known as "calcium-induced calcium release" [10]. Calcium then binds to sarcomeres, the contractile units of the cardiomyocyte.…”

Section: Introductionmentioning

confidence: 99%

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Measuring Single-Cell Calcium Dynamics Using a Myofilament-Localized Optical Biosensor in hiPSC-CMs Derived from DCM Patients

Hawey,

Bourque,

Alim

et al. 2023

Cells

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Synchronized contractions of cardiomyocytes within the heart are tightly coupled to electrical stimulation known as excitation-contraction coupling. Calcium plays a key role in this process and dysregulated calcium handling can significantly impair cardiac function and lead to the development of cardiomyopathies and heart failure. Here, we describe a method and analytical technique to study myofilament-localized calcium signaling using the intensity-based fluorescent biosensor, RGECO-TnT. Dilated cardiomyopathy is a heart muscle disease that negatively impacts the heart’s contractile function following dilatation of the left ventricle. We demonstrate how this biosensor can be used to characterize 2D hiPSC-CMs monolayers generated from a healthy control subject compared to two patients diagnosed with dilated cardiomyopathy. Lastly, we provide a step-by-step guide for single-cell data analysis and describe a custom Transient Analysis application, specifically designed to quantify features of calcium transients. All in all, we explain how this analytical approach can be applied to phenotype hiPSC-CM behaviours and stratify patient responses to identify perturbations in calcium signaling.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Measuring Single-Cell Calcium Dynamics Using a Myofilament-Localized Optical Biosensor in hiPSC-CMs Derived from DCM Patients

Hawey,

Bourque,

Alim

et al. 2023

Cells

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“…During this cycle, the presence of this cation is essential to convert electric stimulation (action potential) in mechanic output (contraction), in a process commonly termed excitation-contraction (EC) coupling (Santulli et al 2017a; Fabiato and Fabiato 1975). The action potential is the electrical signal that depolarizes the plasma membrane of cardiac myocytes, allowing the entrance of a relatively low amount of extracellular Ca 2+ , which in turn induces high Ca 2+ release from the sarcoplasmic reticulum (SR) (Lenzi and Caniggia 1953). Cytosolic Ca 2+ binds to myofilaments, activating contractile machinery (Ebashi et al 1967; Reddy and Honig 1972).…”

Section: Introductionmentioning

confidence: 99%

New Insights in Cardiac Calcium Handling and Excitation-Contraction Coupling

Gambardella

Trimarco

Iaccarino

et al. 2017

Advances in Experimental Medicine and Biology

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Excitation-contraction (EC) coupling denotes the conversion of electric stimulus in mechanic output in contractile cells. Several studies have demonstrated that calcium (Ca2+) plays a pivotal role in this process. Here we present a comprehensive and updated description of the main systems involved in cardiac Ca2+ handling that ensure a functional EC coupling and their pathological alterations, mainly related to heart failure.

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The Electrocardiogram and Disturbances in Electrolyte Balance

Burch¹

1954

Arch Intern Med

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EXCELLENT studies * have resulted in one of the most important recent diagnostic developments in electrocardiography, that is, identification of the electrocardiographic pattern of disturbances in electrolyte equilibrium and metabolism. It has been known for many years that ill health of many types can change the T waves, but interest in disturbances in electrolytes has been limited primarily to calcium. The duration of the Q-T interval is known to vary inversely with the ionic concentration of calcium in the plasma, whereas the configuration of the T wave remains unchanged except for duration.9 Changes in duration of the S-T segment and T wave are primarily responsible for changes in duration of the Q-T interval.10 Until recently, the U wave was considered to possess no clinical significance and was purely of physiologic interest. However, studies in animals and in man by

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Nature of Myocardial Contraction and of Action Potentials. Importance of the »Cationlc Gradient»

Cited by 20 publications

References 19 publications

Measuring Single-Cell Calcium Dynamics Using a Myofilament-Localized Optical Biosensor in hiPSC-CMs Derived from DCM Patients

Measuring Single-Cell Calcium Dynamics Using a Myofilament-Localized Optical Biosensor in hiPSC-CMs Derived from DCM Patients

New Insights in Cardiac Calcium Handling and Excitation-Contraction Coupling

The Electrocardiogram and Disturbances in Electrolyte Balance

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