Nature, Incidence, and Symptomatology of Viruses Infecting Vanilla tahitensis in French Polynesia

Abstract

A survey was carried out to identify the viruses infecting vanilla in French Polynesia and to assess their incidence. Virus identification was based on enzyme-linked immunosorbent assay (ELISA) and, for potyviruses, on the sequence of part of the coat protein and inoculation assays. Between 1998 and 1999, 3,610 vanilla plants from 49 plots in the Society Islands were indexed. Cymbidium mosaic virus (CymMV) was detected in 500 vines from 10 plots in the Leeward Islands. The data suggest that this virus has spread widely since its first detection in French Polynesia in 1986, most likely through the dissemination of symptomless infected cuttings. Viruses belonging to the Potyvirus genus were found in 674 plants from 27 plots in the four islands surveyed. Three distinct potyviruses have been identified: (i) Vanilla mosaic virus, (ii) Watermelon mosaic virus, and (iii) and a virus related to Bean common mosaic virus. The symptoms induced on Vanilla tahitensis by the three potyviruses can be differentiated from each other and from those due to CymMV. A significant proportion of the plants surveyed (97/476) were symptomatic but tested negative by ELISA for CymMV and the Potyvirus group. Odontoglossum ringspot virus was not detected in any sample tested.

Performances and application of antisera produced by recombinant capsid proteins of Cymbidium mosaic virus and Odontoglossum ringspot virus

“…Although there were several reports of CymMV and ORSV surveys in different countries (Wisler et al 1979;Zettler et al 1990;Hu et al 1993;Wong et al 1994;Grisoni et al 2004;Khentry et al 2006), no related information is available in Taiwan. HM-Cy and HM-OR were then used to investigate the infection rate of these two viruses in cultivated Oncidium and Phalaenopsis spp.…”

Identifikasi Molekuler Broad Bean Wilt Virus 2 (Bbwv2) Dan Cymbidium Mosaic Virus (Cymmv) Asal Tanaman Nilam (Pogostemon Cablin Benth.)

“…CymMV telah dilaporkan menginfeksi tanaman anggrek di beberapa negara. Selain tanaman anggrek, CymMV juga dilaporkan menginfeksi tanaman vanili (Grisoni et al, 2004). Di Indonesia, CymMV telah dilaporkan menginfeksi tanaman anggrek (Lakani et al, 2010).…”

Ultrasensitive Detection of Cymbidium Mosaic Potexvirus Using a Single-Wall Carbon Nanotube-Functionalized Quartz Crystal Microbalance

“…The virus reduces plant vigor and flower quality, thus influencing the economic value. 5) A number of methods have been developed to analyze CymMV, e.g., enzyme-linked immunosorbent assay (ELISA), 6,7) liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption-ionization (MALDI), 8) reverse transcription polymerase chain reaction (RT-PCR), 9) immuno-capillary zone electrophoresis (I-CZE), 10) and DIG-labeled cRNA probes. 11) Some of these methods require additional time-consuming labeling and amplification procedures.…”