Nature and bioprospecting of haloalkaliphilics: a review

Uma, G.; Babu, Mariavincent Michael; Prakash, Vincent Samuel Gnana; Nisha, Selvaraj Jeraldin; Citarasu, Thavasimuthu

doi:10.1007/s11274-020-02841-2

Cited by 16 publications

(6 citation statements)

References 162 publications

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“…Sugars (e.g., sucrose and alginate) act as compatible solutes that regulate cellular osmotic pressure. Moreover, such solutes function as stabilizers in the cell, representing an important adaptive mechanism in salt-tolerant bacteria that allows their survival in high-salt environments [ 62 , 63 ]. In addition, sugar is an important energy source and signaling molecule involved in the metabolic activity of microorganisms [ 64 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

Integrated Genomics and Transcriptomics Provide Insights into Salt Stress Response in Bacillus subtilis ACP81 from Moso Bamboo Shoot (Phyllostachys praecox) Processing Waste

Li,

Huang,

Zhong

et al. 2024

Microorganisms

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Salt stress is detrimental to the survival of microorganisms, and only a few bacterial species produce hydrolytic enzymes. In this study, we investigated the expression of salt stress-related genes in the salt-tolerant bacterial strain Bacillus subtilis ACP81, isolated from bamboo shoot processing waste, at the transcription level. The results indicate that the strain could grow in 20% NaCl, and the sub-lethal concentration was 6% NaCl. Less neutral protease and higher cellulase and β-amylase activities were observed for B. subtilis ACP81 under sub-lethal concentrations than under the control concentration (0% NaCl). Transcriptome analysis showed that the strain adapted to high-salt conditions by upregulating the expression of genes involved in cellular processes (membrane synthesis) and defense systems (flagellar assembly, compatible solute transport, glucose metabolism, and the phosphotransferase system). Interestingly, genes encoding cellulase and β-amylase-related (malL, celB, and celC) were significantly upregulated and were involved in starch and sucrose metabolic pathways, and the accumulated glucose was effective in mitigating salt stress. RT-qPCR was performed to confirm the sequencing data. This study emphasizes that, under salt stress conditions, ACP81 exhibits enhanced cellulase and β-amylase activities, providing an important germplasm resource for saline soil reclamation and enzyme development.

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Section: Discussionmentioning

confidence: 99%

Integrated Genomics and Transcriptomics Provide Insights into Salt Stress Response in Bacillus subtilis ACP81 from Moso Bamboo Shoot (Phyllostachys praecox) Processing Waste

Li,

Huang,

Zhong

et al. 2024

Microorganisms

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“…The following supporting information can be downloaded at: https:// www.mdpi.com/article/10.3390/microorganisms11102475/s1. Table S1: Information on sediment samples collected from four saline lakes; Table S2: Compositions of the media used in this study; Table S3: Taxonomic statistics of the isolated 255 actinobacterial strains; Table S4: Antibacterial profile of 46 bioactive strains isolated from the sediments of four saline lakes on the Northern Tibetan Plateau; Table S5: Antimicrobial activities of crude extracts from two selected streptomyces strains 378 and 549; Table S6: Putatively identified metabolites from liquid culture extracts of strains 378 and 549; Table S7: Biosynthesis gene clusters of secondary metabolites from strain 378 detected by anti-SMASH; Table S8: Biosynthesis gene clusters of secondary metabolites from strain 549 detected by anti-SMASH; Figure S1: Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences of strain LD-9 (1509 bp) and related type species of the family Microbacteriaceae; Figure S2: UPLC-MS/MS spectra of putative cosmomycin compounds (7)(8)(9)(10)(11)(12)(13)(14)(15) from strain 378 acquired by MS E method; Figure S3: UPLC-MS/MS spectra of putative kidamycin derivatives (16)(17)(18)(19)(20)(21)…”

Section: Supplementary Materialsmentioning

confidence: 99%

“…[11]. However, the antibiotic discovery of actinobacteria isolated from saline lakes has thus far been minimal, which needs more research to focus on the source [12,13].…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting of Actinobacterial Diversity and Antibacterial Secondary Metabolites from the Sediments of Four Saline Lakes on the Northern Tibetan Plateau

Liu,

Zhai,

Liu

et al. 2023

Microorganisms

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The Tibetan Plateau, known as the “Roof of the World” and “The Third Pole”, harbors numerous saline lakes primarily distributed in the Northern Tibetan Plateau. However, the challenging conditions of high altitude, low oxygen level, and harsh climate have limited investigations into the actinobacteria from these saline lakes. This study focuses on investigating the biodiversity and bioactive secondary metabolites of cultivable actinobacteria isolated from the sediments of four saline lakes on the Northern Tibetan Plateau. A total of 255 actinobacterial strains affiliated with 21 genera in 12 families of 7 orders were recovered by using the pure culture technique and 16S rRNA gene phylogenetic analysis. To facilitate a high-throughput bioactivity evaluation, 192 isolates underwent OSMAC cultivation in a miniaturized 24-well microbioreactor system (MATRIX cultivation). The antibacterial activity of crude extracts was then evaluated in a 96-well plate antibacterial assay. Forty-six strains demonstrated antagonistic effects against at least one tested pathogen, and their underlying antibacterial mechanisms were further investigated through a dual-fluorescent reporter assay (pDualrep2). Two Streptomyces strains (378 and 549) that produce compounds triggering DNA damage were prioritized for subsequent chemical investigations. Metabolomics profiling involving HPLC-UV/vis, UPLC-QTOF-MS/MS, and molecular networking identified three types of bioactive metabolites belonging to the aromatic polyketide family, i.e., cosmomycin, kidamycin, and hedamycin. In-depth analysis of the metabolomic data unveiled some potentially novel anthracycline compounds. A genome mining study based on the whole-genome sequences of strains 378 and 549 identified gene clusters potentially responsible for cosmomycin and kidamycin biosynthesis. This work highlights the effectiveness of combining metabolomic and genomic approaches to rapidly identify bioactive chemicals within microbial extracts. The saline lakes on the Northern Tibetan Plateau present prospective sources for discovering novel actinobacteria and biologically active compounds.

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“…Extreme environments have shaped biodiversity driven by harsh physicochemical conditions displayed in a myriad of adaptations in microorganisms of high biotechnological value, including microbial enzymes for a plethora of applications ( Manni & Filali-Maltouf, 2022 ; Pascoal, Magalhaes & Costa, 2020 ; Sysoev et al, 2021 ; Tatta et al, 2022 ; Uma et al, 2020 ; Wani et al, 2022a ). Bacterial enzymes possess key features more advantageous than fungal enzymes such as higher growth rates, genetic versatility ( Menendez, Garcia-Fraile & Rivas, 2015 ) and more specific, efficient catalytic activity in harsh conditions; moreover, bacteria produce multienzyme complexes with greater functionality and specificity in broader conditions ( Balla et al, 2022 ; Castiglia et al, 2016 ; Maki, Leung & Qin, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Systematic bioprospection for cellulolytic actinomycetes in the Chihuahuan Desert: isolation and enzymatic profiling

Escudero-Agudelo,

Martínez-Villalobos,

Arocha-Garza

et al. 2023

PeerJ

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The quest for microbial cellulases has intensified as a response to global challenges in biofuel production. The efficient deconstruction of lignocellulosic biomass holds promise for generating valuable products in various industries such as food, textile, and detergents. This article presents a systematic bioprospection aimed at isolating actinomycetes with exceptional cellulose deconstruction capabilities. Our methodology explored the biodiverse oligotrophic region of Cuatro Cienegas, Coahuila, within the Chihuahuan Desert. Among the evaluated actinomycetes collection, 78% exhibited cellulolytic activity. Through a meticulous screening process based on enzymatic index evaluation, we identified a highly cellulolytic Streptomyces strain for further investigation. Submerged fermentation of this strain revealed an endoglucanase enzymatic activity of 149 U/mg. Genomic analysis of strain Streptomyces sp. STCH565-A revealed unique configurations of carbohydrate-active enzyme (CAZyme) genes, underscoring its potential for lignocellulosic bioconversion applications. These findings not only highlight the significance of the Chihuahuan Desert as a rich source of cellulolytic microorganisms but also offer insights into the systematic exploration and selection of high-performing cellulolytic microorganisms for application in diverse environmental contexts. In conclusion, our bioprospecting study lays a foundation for harnessing the cellulolytic potential of actinomycetes from the Chihuahuan Desert, with implications for advancing cellulose deconstruction processes in various industries. The findings can serve as a blueprint for future bioprospecting efforts in different regions, facilitating the targeted discovery of microorganisms with exceptional cellulosic deconstruction capabilities.

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Nature and bioprospecting of haloalkaliphilics: a review

Cited by 16 publications

References 162 publications

Integrated Genomics and Transcriptomics Provide Insights into Salt Stress Response in Bacillus subtilis ACP81 from Moso Bamboo Shoot (Phyllostachys praecox) Processing Waste

Integrated Genomics and Transcriptomics Provide Insights into Salt Stress Response in Bacillus subtilis ACP81 from Moso Bamboo Shoot (Phyllostachys praecox) Processing Waste

Bioprospecting of Actinobacterial Diversity and Antibacterial Secondary Metabolites from the Sediments of Four Saline Lakes on the Northern Tibetan Plateau

Systematic bioprospection for cellulolytic actinomycetes in the Chihuahuan Desert: isolation and enzymatic profiling

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