Nature and Action of a New Clotting Factor Obtained From Erythrocytes

Georgatsos, John G.; Hussey, Clara V.; Quick, Armand J.

doi:10.1152/ajplegacy.1955.181.1.30

Cited by 28 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The prothrombin utilization of blood from mild cases of haemophilia to which haemolysate was added before clotting was restored to normal, but it was only improved, though remaining abnormal, in blood from patients with severe haemophilia or Christmas disease (Table III). These results agree with the observations of Georgatsos et al (1955) and Izarn et al (1955) that the thrombin generation of a haemophilic plasma is increased when haemolysate is substituted for platelets in the test of Pitney and Dacie (1953) (Table IV). Antiheparin Activity.-Lysed erythrocytes show a marked antiheparin activity comparable to the activity of a concentrated platelet suspension, and it is directly proportional to the concentration of haemolysate and not inhibited by an excess.…”

supporting

confidence: 92%

“…It was also shown by Leupold (1955), Georgatsos et al (1955), and Bounameaux (1957a) that lysed red cells can be substituted for platelets in the thromboplastin generation test of Biggs and Douglas (1953).…”

mentioning

confidence: 91%

“…Thromboplastic activity is not produced by whole erythrocytes, but only after lysis by freezing or dilution with distilled water. Lysed erythrocytes increase the prothrombin consumption of plateletpoor or platelet-rich plasma and restore to normal the prolonged recalcification time and the impaired prothrombin consumption of a thrombocytopenic plasma (Georgatsos et al, 1955). The E.C.F.…”

mentioning

confidence: 99%

“…A thromboplastic factor has been demonstrated in human red cells by Shinowara (1951), Ottaviani, Dettori, and Manai (1954), Quick, Georgatsos, and Hussey (1954), Leupold (1955), Bounameaux (1957a), and Georgatsos, Hussey, and Quick (1955), and named the "erythrocyte clotting factor" (E.C.F.). Its activity is similar though not identical with that of platelet factor 3.…”

mentioning

confidence: 99%

See 3 more Smart Citations

The Coagulation Properties of Human Erythrocytes

Serafini¹,

Centurelli²

1959

J Clin Pathol

View full text Add to dashboard Cite

supporting

confidence: 92%

mentioning

confidence: 91%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

The Coagulation Properties of Human Erythrocytes

Serafini¹,

Centurelli²

1959

J Clin Pathol

View full text Add to dashboard Cite

“…En effet, lors de l'hémolyse, il y a libération de potassium (Mincev [31]), d'ATP (Studer et al [41]), de substances histamino-semblables (Barsoom et Smirk [5]) et de plusieurs facteurs intervenant dans la coagulation (Stanski et Quick [40], Georgatsos et al [16], Ottaviani et al [35]) parmi lesquels le facteur R serait res ponsable pour Hellem [17] de la baisse des thrombocytes observée dans les perfusions.…”

Section: Méthodeunclassified

Influences sur le sang de matériaux utilisables en circulation extra-corporelle

Duc¹

1962

Vox Sanguinis

View full text Add to dashboard Cite

Hemophilia: Quantitative Studies of the Coagulation Defect

Quick¹

1956

AMA Arch Intern Med

View full text Add to dashboard Cite

The prothrombin-consumption test described by one of us in 1947 1 was based on the assumption that the amount of prothrombin remaining in serum when blood clotted under standardized conditions was a measure of the thromboplastin that resulted from the interaction of platelets with a plasma constituent which was designated as thromboplastinogen. It was concluded that the poor consumption of prothrombin observed when hemophilic blood clotted was caused by a lack of this plasma factor and that, therefore, the method could be employed for its assay. This assumption was supported by the significant increase in prothrombin consumption obtained after giving a hemophiliac a transfusion of normal blood or plasma. In mild hemophilia, however, the results were not always consistent, and, peculiarly, it was observed that when the platelet-rich plasma of such patients was clotted the prothrombin consumption was lower and more constant than when the test was done on whole blood.2This observation led to the discovery that the erythrocytes contain a potent clotting factor which is distinct from all the known primary clotting agents of the blood.3 The name "erythrocytin" is proposed for this substance.When the erythrocyte factor is added to normal plasma depleted of platelets, an ex¬ ceedingly high consumption of prothrombin is obtained, which shows not only that the new agent can completely replace platelets but also that it is more potent than the latter. Strikingly, no measurable amount of pro¬ thrombin consumption occurs when an ex¬ tract of hemolyzed erythrocytes (hemolysate) or a partially purified preparation of erythro¬ cytin is added to the blood or plasma of a severe hemophiliac. When a purified prepa¬ ration of thromboplastinogen is added to hémophilie blood or plasma and the pro¬ thrombin consumption with a fixed excess of hemolysate is determined, a straight-line proportion between the concentration of thromboplastinogen and the prothrombinconsumption time is obtained.4 The possi¬ bility of utilizing this observation for the development of a method to assay blood for its concentration of thromboplastinogen was investigated, and the results of this study, especially as it pertains to hemophilia, are reported in this paper. METHODS AND MATERIALSBlood was collected with a silicone-coated syringe and needle and transferred to containers similarly coated which were immersed and kept in an ice bath.The clotting time was carried out by standardized procedure previously described.5 The normal range is 5 to 10 minutes.

show abstract

Nature and Action of a New Clotting Factor Obtained From Erythrocytes

Cited by 28 publications

References 0 publications

The Coagulation Properties of Human Erythrocytes

The Coagulation Properties of Human Erythrocytes

Influences sur le sang de matériaux utilisables en circulation extra-corporelle

Hemophilia: Quantitative Studies of the Coagulation Defect

Contact Info

Product

Resources

About