Naturally-occurring human serum antibodies to inner core lipopolysaccharide epitopes of Neisseria meningitidis protect against invasive meningococcal disease caused by isolates displaying homologous inner core structures

Jäkel, Anne; Plested, Joyce S.; Hoe, J. Claire; Makepeace, Katherine; Gidney, Margaret-Anne J.; Lacelle, Suzanne; Michael, Frank St.; Cox, Andrew D.; Richards, James C.; Moxon, E. Richard

doi:10.1016/j.vaccine.2008.09.041

Cited by 22 publications

(21 citation statements)

References 51 publications

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“…Their immunochemical data also indicate that affinity-purified IgGs are bactericidal and bind to the OS moieties of the 3,4-branched LOSs. These recent data by us 3,7) and other investigators 5,6) show that NHS contains anti-LOS Abs that are specific for the OS of LOS. They also suggest that NHS may contains numerous unidentified anti-LOS antibodies that recognize the OS moiety of LOS.…”

mentioning

confidence: 59%

“…The results will be published elsewhere. As described earlier, recent studies have shown that NHS contains bactericidal anti-LOS IgGs, 3,[5][6][7] but the specificities of these anti-LOS IgGs have not yet been fully characterized. Characterization of such antibodies should help not only to understand the immune responses of the host against pathogenic Neisseria species, but also to develop effective carbohydratebased vaccines against those bacteria.…”

Section: Nhs-igg Bound To the Os Moiety Of Neisserial Loss And Salmonmentioning

confidence: 94%

“…This anti-15253 LOS IgG contained at least three species of IgG2, and each of the three IgG2s was found to recognize a different OS structure expressed in the 2,3:3,4-dibranched 15253 LOS. Other investigators 5,6) have also purified anti-LOS human IgGs using N. meningitidis LOSs. Their immunochemical data also indicate that affinity-purified IgGs are bactericidal and bind to the OS moieties of the 3,4-branched LOSs.…”

mentioning

confidence: 99%

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Western Blot Analysis of Neisserial Lipooligosaccharide at the Lower Femtomole Level with Normal Human Sera

Yamasaki

Ikezaki

Seo

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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mentioning

confidence: 59%

Section: Nhs-igg Bound To the Os Moiety Of Neisserial Loss And Salmonmentioning

confidence: 94%

mentioning

confidence: 99%

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Western Blot Analysis of Neisserial Lipooligosaccharide at the Lower Femtomole Level with Normal Human Sera

Yamasaki

Ikezaki

Seo

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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“…However, most anti-LPS antibodies in normal human sera have likely developed after exposure to antigen, either from pathogenic or commensal organisms ("naturally induced" antibodies) (14,23,40) or as a result of immunization (2,11,12,39,54,65).…”

Section: Discussionmentioning

confidence: 99%

“…The presence of bactericidal antibodies to meningococcal LPS in human sera has been demonstrated by several investigators (13,19,23,54). These antibodies are typically specific for the wild-type LPS that contains the LNnT.…”

mentioning

confidence: 94%

Importance of Antibodies to Lipopolysaccharide in Natural and Vaccine-Induced Serum Bactericidal Activity against Neisseria meningitidis Group B

et al. 2011

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Analysis of the specificity of bactericidal antibodies in normal, convalescent, and postvaccination human sera is important in understanding human immunity to meningococcal infections and can aid in the design of an effective group B vaccine. A collection of human sera, including group C and group B convalescent-phase sera, normal sera with naturally occurring cross-reactive bactericidal activity, and some postvaccination sera, was analyzed to determine the specificity of cross-reactive bactericidal antibodies. Analysis of human sera using a bactericidal antibody depletion assay demonstrated that a significant portion of the bactericidal activity could be removed by purified lipopolysaccharide (LPS). LPS homologous to that expressed on the bactericidal test strain was most effective, but partial depletion by heterologous LPS suggested the presence of antibodies with various degrees of cross-reactivity. Binding of anti-L3,7 LPS bactericidal antibodies was affected by modification of the core structure, suggesting that these functional antibodies recognized epitopes consisting of both core structures and lacto-N-neotetraose (LNnT). When the target strain was grown with 5-cytidinemonophospho-N-acetylneuraminic acid (CMP-NANA) to increase LPS sialylation, convalescentphase serum bactericidal titers were decreased by only 2-to 4-fold, and most remaining bactericidal activity was still depleted by LPS. Highly sialylated LPS was ineffective in depleting bactericidal antibodies. We conclude that natural infections caused by strains expressing L3,7 LPS induce persistent, protective bactericidal antibodies and appear to be directed against nonsialylated bacterial epitopes. Additionally, subsets of these bactericidal antibodies are cross-reactive, binding to several different LPS immunotypes, which is a useful characteristic for an effective group B meningococcal vaccine antigen.

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Aggregation of monocytes and platelets interferes in measurement of monocyte viability with phosphatidylserine expression but not with Mitochondrial membrane potential in whole blood

et al. 2015

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Naturally-occurring human serum antibodies to inner core lipopolysaccharide epitopes of Neisseria meningitidis protect against invasive meningococcal disease caused by isolates displaying homologous inner core structures

Cited by 22 publications

References 51 publications

Western Blot Analysis of Neisserial Lipooligosaccharide at the Lower Femtomole Level with Normal Human Sera

Western Blot Analysis of Neisserial Lipooligosaccharide at the Lower Femtomole Level with Normal Human Sera

Importance of Antibodies to Lipopolysaccharide in Natural and Vaccine-Induced Serum Bactericidal Activity against Neisseria meningitidis Group B

Aggregation of monocytes and platelets interferes in measurement of monocyte viability with phosphatidylserine expression but not with Mitochondrial membrane potential in whole blood

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