Naturally occurring bovine leukosis virus in water buffalo (<i>Bubalus bubalis</i>) in Brazil

Molnár, Éva; Molnár, L.; Guedes, V. T. M.; Lima, Elaine S.

doi:10.1136/vr.146.24.705

Cited by 9 publications

(3 citation statements)

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“…En este estudio se encontró un 24.6 % de animales positivos por ELISA, pero ninguno positivo por AGID que es la prueba considerada como la Gold estándar; tampoco se obtuvieron resultados positivos por medio de PCR, por lo que se concluyó que no había presencia del virus en los animales evaluados; sin embargo, en el año 2000 se encontró en la misma región amazónica la presencia de anticuerpos al VLB en búfalos. Las pruebas que se realizaron fueron AGID y dos tipos de ELISA para detección de antígeno gp51 e inmunoglobulina G. La conclusión de ese estudio fue la presencia natural del virus en búfalos (31) con 87, 81 y 69 muestras de suero positivas por cada prueba, respectivamente.…”

Section: Discussionunclassified

Prevalencia del virus de la leucosis bovina en búfalos de agua en la región centro occidental de Colombia

Florez

Marín

et al. 2021

Rev. Mex. Cienc. Pecu.

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La leucosis viral bovina (LVB) es una enfermedad de alta morbilidad y baja mortalidad. Existen reportes de la infección natural en algunas especies de bóvidos; sin embargo, en búfalos es poco estudiado. En Colombia la producción de búfalos crece rápidamente y hasta el momento no hay reportes de la enfermedad. El objetivo de este trabajo fue caracterizar la producción bufalina en el eje cafetero y determinar la prevalencia de LVB por PCR en búfalos y la presencia en humanos, bovinos y ovejas en cercanías. Se tomaron muestras de sangre de 140 búfalos, 10 de leche de búfala y 58 muestras de bovinos, 35 de ovejas y 9 de humanos en contacto con los búfalos. Se realizaron análisis hematológicos. Posteriormente, extracción de ADN para evaluación por PCR. Se recolectó información productiva y los resultados fueron procesados y analizados mediante el software R. La mayoría de animales fueron hembras de la raza mediterránea, con peso al nacimiento de 33.39 kg, peso al destete 202.93 kg, intervalo entre partos 491.77 días, tiempo al pico 67.26 días y 381.59 L de leche (ajustada a 305 días a dos tetas). Se encontró una prevalencia de 33.6 % en búfalos y 3.4 % en bovinos; ninguna muestra de leche, oveja o humano resultó positiva. No se encontraron factores de riesgo asociados a la infección, ni una alteración significativa del cuadro hemático, ni de los factores productivos. Estos resultados se constituyen en el primer reporte molecular del VLB en América y una de las primeras en el mundo.

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Section: Discussionunclassified

Prevalencia del virus de la leucosis bovina en búfalos de agua en la región centro occidental de Colombia

Florez

Marín

et al. 2021

Rev. Mex. Cienc. Pecu.

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“…No Brasil, mais precisamente no Estado do Maranhão, foram encontrados 4,2% (10/232) de búfalos soropositivos para o BLV [9]. Aproximadamente 568 amostras de búfalos criados do Estado do Pará apresentaram soropositividade após a inoculação com sangue bovino, com prevalência que variaram de 24,6% (IDGA) a 42,2% (ELISA -2; Kit ELISA Leucose modificado com Imunoglobulina G anti-bovina) [27].…”

Section: Discussionunclassified

Bovine Leukosis Virus and Antibodies against Bovine Herpesvirus Type-1 and Bovine Viral Diarrhea Virus and in Buffaloes of the State of Pará, Brazil

Viana

Fava

Moura³

et al. 2016

Acta Scientiae. Vet.

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Background: Viral diseases affecting reproduction cause economic losses in cattle, as reproductive failure interrupts the production cycle, thus reducing herd productivity. Buffaloes are susceptible to most of the diseases that affect cattle. Some of the viral diseases of reproductive importance are infectious bovine rhinotracheitis (IBR), bovine viral diarrhea (BVD), and bovine leukosis (BL). The objective of this study was to determine the seroprevalence of IBR virus (bovine herpes virus-1, BoHV-1), BVD virus (BVDV), and BL virus (BLV) in female buffaloes living in wetland areas or plains in the state of Pará, as well as the seroprevalence of BoHV-1, BVDV, and BLV in females of different age groups.Materials, Methods & Results: It were used 225 crossbred buffaloes from 4 buffalo exclusive farms. The buffaloes reared on farms A (n = 50) and B (n = 89) were kept in wetland areas on Marajó Island (n = 139), Pará. On farms C (n = 30) and D (n = 56) buffaloes were kept in plains (n = 86) in a northeastern mesoregion of Para. Animals were categorized into age groups: I: 0-3 years (n = 34), II: 3-6 years (n = 58), III: 7-9 years (n = 55), and IV: over 9 years (n = 78). Blood samples were collected and sera were sent for processing at the Bovine Virus Laboratory, Biological Institute of São Paulo, Brazil. The presence of IBRV and BVDV was determined by neutralization and BLV antibodies by immunodiffusion in agar gel. Statistical analysis was performed using the Ȥ2 test at a signiﬁcance level of 5%. Among the seroprevalences of IBRV, BVDV and BLV antibodies, the difference in prevalence for BVD [P < 0.01] was observed between wetland areas and plains of Para, varying from 12.4% to 96.0% and 0.0% to 13.3%, respectively. While levels of IBRV remained high on farms A (79.6%), B (86.5%), C (83.3%) and D (89.1%) [P = 0.60] and BLV was negative in all the animals. BVDV was similar for all the age groups [P = 0.60], while IBRV was more prevalent in animals over 6 years of age with group I: (76.5%), II (74.5%), III (92.3%) and IV (91.3%) [P = 0.01]. It was not possible verify the inﬂuence of age in prevalence for BL.Discussion: The prevalence obtained for the BoHV-1 on farms was high, with little variation between 79.6% and 89.1%. The high prevalence of BoHV-1 suggests the free circulation of the virus in the state of Para. Notably, the animals in this study had not been vaccinated, so the determined viral antibody titers were independent of vaccination. BVDV results showed high variation between 0.0% and 96.0%. The highest prevalence in wetland areas may be related to the breeding environment, because of a possible horizontal contamination. The lack of BLV antibodies prevalence can be attributed to the fact that all the evaluated animals were kept in an extensive breeding system where they had little direct contact. The prevalence of IBR was higher in the older animals. Variation in prevalence with age was not observed for BVD. It was concluded that the prevalence for IBR and BVD were high indicating that the etiological agents are circulating in Para, with IBR having a higher prevalence in older animals. Breeding buffalo in wetland areas may favor horizontal transmission of BVDV because facilitate contact of healthy animals with water contaminated by virus carriers animal secretion and the absence of seropositive animals at the BLV does not necessarily indicate that buffaloes are resistant to the virus.

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“…EBL occurs mainly in adult cattle (Bos taurus), but other species are susceptible to experimental infection (e.g., sheep-Ovis aries) [3] and infection under natural conditions (e.g., water buffalo-Bubalus bubalis) [4,5]. Moreover, the potential risks of BLV for human health were recently confirmed, because the virus was detected in the breast tissue and blood of Iranian women using nested PCR [6], although there is no other robust evidence of human infection with EBL.…”

Section: Introductionmentioning

confidence: 99%

Enzootic Bovine Leukosis in Italy: Epidemiological Issues after Free Status Recognition and Measures Applied to Tackle the Last Persistent Clusters

et al. 2021

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Enzootic Bovine Leukosis (EBL), caused by the bovine leukemia virus (BLV), has been eradicated in over 20 countries, most of which are in Western Europe. The European Commission, in 2017, declared Italy to be an officially EBL-free country by means of Commission Implementing Decision (EU) 2017/1910, despite the presence of some infection clusters located in four regions of Central-Southern Italy. As a consequence of persisting infection, the Italian Ministry of Health established specific eradication measures in these areas. In collaboration with the National Reference Laboratory for the Study of Ruminant Retroviral Infectious Diseases, the Ministry of Health employed data from the veterinary information system digital platform, combined with a gap analysis exercise, to monitor and verify the progress of control activities within infection clusters during the period 2018–2021. Our aim was to identify any remaining gaps and, consequently, specific measures to eliminate the factors favouring EBL persistence, on the basis of a description and analysis of the current data regarding epidemiological trends in Italian clusters. The final goal is to achieve the implementation of a less expensive surveillance plan in these areas, as well. The results of comprehensive analysis showed that the eradication activities had been effectively implemented by official local veterinary services, resulting in a drastic reduction of EBL outbreaks in most territories during the period 2018–2021.

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Naturally occurring bovine leukosis virus in water buffalo (Bubalus bubalis) in Brazil

Cited by 9 publications

References 3 publications

Prevalencia del virus de la leucosis bovina en búfalos de agua en la región centro occidental de Colombia

Prevalencia del virus de la leucosis bovina en búfalos de agua en la región centro occidental de Colombia

Bovine Leukosis Virus and Antibodies against Bovine Herpesvirus Type-1 and Bovine Viral Diarrhea Virus and in Buffaloes of the State of Pará, Brazil

Enzootic Bovine Leukosis in Italy: Epidemiological Issues after Free Status Recognition and Measures Applied to Tackle the Last Persistent Clusters

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