Naturally occurring aliphatic polyamines‐induced histamine release from rat peritoneal mast cells

Kurosawa, Motohiro; Uno, Daisuke; Kobayashi, Setsuo

doi:10.1111/j.1398-9995.1991.tb00597.x

Cited by 13 publications

(14 citation statements)

References 13 publications

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“…Polyamines are required for DNA synthesis and cell proliferation (58,59), and for synthesis of proline, the precursor for collagen production (60,61). Consistent with the finding of greater arginase activity in asthma in the current study, serum levels of polyamines are elevated in subjects with asthma and in experimental models of asthma (5,(62)(63)(64)(65). Taken together with previous studies, the present cross-sectional observational study identifies alterations of Arg metabolism in asthma.…”

Section: Discussionsupporting

confidence: 79%

Alterations of the Arginine Metabolome in Asthma

Lara¹,

Khatri²,

Wang³

et al. 2008

Am J Respir Crit Care Med

118

100

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Rationale: As the sole nitrogen donor in nitric oxide (NO) synthesis and key intermediate in the urea cycle, arginine and its metabolic pathways are integrally linked to cellular respiration, metabolism, and inflammation. Objectives: We hypothesized that arginine (Arg) bioavailability would be associated with airflow abnormalities and inflammation in subjects with asthma, and would be informative for asthma severity. Methods: Arg bioavailability was assessed in subjects with severe and nonsevere asthma and healthy control subjects by determination of plasma Arg relative to its metabolic products, ornithine and citrulline, and relative to methylarginine inhibitors of NO synthases, and by serum arginase activity. Inflammatory parameters, including fraction of exhaled NO (FE NO ), IgE, skin test positivity to allergens, bronchoalveolar lavage, and blood eosinophils, were also evaluated. Measurements and Main Results: Subjects with asthma had greater Arg bioavailability, but also increased Arg catabolism compared with healthy control subjects, as evidenced by higher levels of FE NO and serum arginase activity. However, Arg bioavailability was positively associated with FE NO only in healthy control subjects; Arg bioavailability was unrelated to FE NO or other inflammatory parameters in severe or nonsevere asthma. Inflammatory parameters were related to airflow obstruction and reactivity in nonsevere asthma, but not in severe asthma. Conversely, Arg bioavailability was related to airflow obstruction in severe asthma, but not in nonsevere asthma. Modeling confirmed that measures of Arg bioavailabilty predict airflow obstruction only in severe asthma. Conclusions: Unlike FE NO , Arg bioavailability is not a surrogate measure of inflammation; however, Arg bioavailability is strongly associated with airflow abnormalities in severe asthma.

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Section: Discussionsupporting

confidence: 79%

Alterations of the Arginine Metabolome in Asthma

Lara¹,

Khatri²,

Wang³

et al. 2008

Am J Respir Crit Care Med

118

100

View full text Add to dashboard Cite

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“…Recent investigations from our laboratory indicate the presence of increased polyphosphoinositide metabolism in activated rat mast cells (5). Moreover, it has been demonstrated that naturally occurring aliphatic polyamines, such as spermine and spermidine, stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules (6) and induce histamine release from rat mast cells (7). Interestingly, spermine at a concentration of 0.1 nimol/1, which in itself could not cause a significant histamine release, showed a tendency to enhance anti-IgEinduced histamine release from mast cells (7).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, it has been demonstrated that naturally occurring aliphatic polyamines, such as spermine and spermidine, stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules (6) and induce histamine release from rat mast cells (7). Interestingly, spermine at a concentration of 0.1 nimol/1, which in itself could not cause a significant histamine release, showed a tendency to enhance anti-IgEinduced histamine release from mast cells (7). These findings, taken together with the widely accepted idea that mast cells are involved mainly in the immediate asthmatic response (3), led us to investigate the levels of peripheral blood polyamines among the same bronchial asthmatic patients with and without attacks.…”

Section: Discussionmentioning

confidence: 99%

“…Recent investigations from our laboratory indicate that naturally occurring ahphatic polyamines, such as spermine and spermidine, stimulate the phosphatidyhnositol kinase that catalyzes the formation of phosphatidyhnositol 4-phosphate from endogenous phosphatidyhnositol in rat peritoneal mast cell granules (6). They also induce a significant histamine release from rat peritoneal mast cells (7), suggesting possible involvement of polyamines in allergic reactions. These findings made us investigate the levels of peripheral-blood, naturally occurring aliphatic polyamines in bronchial asthmatic patients, and the present study indicates a possible role for the polyamines in bronchial asthma.…”

mentioning

confidence: 98%

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Elevated levels of peripheral‐blood, naturally occurring aliphatic polyamines in bronchial asthmatic patients with active symptoms

Kurosawa

Shimizu

Tsukagoshi

et al. 1992

Allergy

Self Cite

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The levels of peripheral-blood, naturally occurring aliphatic polyamines, such as putrescine, spermidine, and spermine, from 21 bronchial asthmatic patients (11 atopics and 10 nonatopics) were measured by postcolumn derivatization high-performance liquid chromatography analysis. None of the patients, except the 44-year-old woman in the case report below, were given prednisolone, and they were instructed to take only regular medication during the tests. Blood was drawn from the patients in a fasting state, and the polyamine levels were compared between the times when they were free of asthmatic symptoms and when they had mild spontaneous attacks. Nine (5 atopics and 4 nonatopics), 6 (3 atopics and 3 nonatopics), and 4 (3 atopics and 1 nonatopic) out of 20 patients, when they had relatively mild asthmatic attacks, showed higher putrescine, spermidine, and spermine levels, respectively, than those of normal healthy control subjects. The levels of peripheral blood polyamines from a 44-year-old atopic bronchial asthmatic woman, who was admitted to the hospital with severe asthmatic attacks, were measured serially, and the putrescine and spermidine levels were found to be elevated during the asthmatic attacks, returning to normal levels in parallel with the clinical course. These data may suggest a role for naturally occurring aliphatic polyamines in bronchial asthma.

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“…These polyamines are stored in mast cell secretory granules, appear in the extracellular space through A23187-evoked Ca 2ϩ -dependent processes, and are thought to be involved in biogenesis and homeostasis of secretory granules (4). Furthermore, it has been reported that spermine and spermidine, when applied to mast cells extracellularly, facilitate synthesis and release of histamine from mast cells (7)(8)(9)(10)(11). Spermine and spermidine are also involved in storage and/or release of histamine and serotonin in the mast cell granules (4,12).…”

mentioning

confidence: 99%

Vesicular Polyamine Transporter Mediates Vesicular Storage and Release of Polyamine from Mast Cells

Takeuchi

Harada

Moriyama

et al. 2017

Journal of Biological Chemistry

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Edited by Luke O'Neillrelease, which was shown to be temperature-dependent and sensitive to bafilomycin A 1 and tetanus toxin, was significantly suppressed by VPAT gene RNA interference. Under these conditions, expression of vesicular monoamine transporter 2 was unaffected, but antigen-dependent histamine release was significantly suppressed, which was recovered by the addition of 1 mM spermine. These results strongly suggest that VPAT is expressed and is responsible for vesicular storage of spermine and spermidine in novel secretory granules that differ from histamine-and serotonin-containing granules and is involved in vesicular release of these polyamines from mast cells.

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Naturally occurring aliphatic polyamines‐induced histamine release from rat peritoneal mast cells

Cited by 13 publications

References 13 publications

Alterations of the Arginine Metabolome in Asthma

Alterations of the Arginine Metabolome in Asthma

Elevated levels of peripheral‐blood, naturally occurring aliphatic polyamines in bronchial asthmatic patients with active symptoms

Vesicular Polyamine Transporter Mediates Vesicular Storage and Release of Polyamine from Mast Cells

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