Natural variation of rice blast resistance gene Pi-d2

Li, J.B.; Sun, Yanxia; Liu, Hongbin; Wang, Y.Y.; Jia, Yulin; Xu, Minghui

doi:10.4238/2015.february.13.2

Cited by 21 publications

(16 citation statements)

References 29 publications

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“…In Haynaldia villosa , transgenic plants overexpressing L-type LecRLK ( LecRK - V ) were more affected by wheat powdery mildew [ 38 ]. Similarly, rice Pi-d2 (G-type LecRLK) has been found to induce the plant’s defense response against fungal pathogens [ 39 ], which is in agreement with our findings. These results broaden our understanding of the critical role of cucumber LecRLKs in plant-fungal interaction.…”

Section: Discussionsupporting

confidence: 92%

Genome-Wide Identification, Diversification, and Expression Analysis of Lectin Receptor-Like Kinase (LecRLK) Gene Family in Cucumber under Biotic Stress

Haider

Britto

Nagaraj

et al. 2021

IJMS

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Members of the lectin receptor-like kinase (LecRLKs) family play a vital role in innate plant immunity. Few members of the LecRLKs family have been characterized in rice and Arabidopsis, respectively. However, little literature is available about LecRLKs and their role against fungal infection in cucumber. In this study, 60 putative cucumber LecRLK (CsLecRLK) proteins were identified using genome-wide analysis and further characterized into L-type LecRLKs (24) and G-type LecRLKs (36) based on domain composition and phylogenetic analysis. These proteins were allocated to seven cucumber chromosomes and found to be involved in the expansion of the CsLecRLK gene family. Subcellular localization of CsaLecRLK9 and CsaLecRLK12 showed green fluorescence signals in the plasma membrane of leaves. The transcriptional profiling of CsLecRLK genes showed that L-type LecRLKs exhibited functional redundancy as compared to G-type LecRLKs. The qRT-PCR results indicated that both L- and G-type LecRLKs showed significant response against plant growth-promoting fungi (PGPF- Trichoderma harzianum Rifai), powdery mildew pathogen (PPM—Golovinomyces orontii (Castagne) V.P. Heluta), and combined (PGPF+PPM) treatments. The findings of this study contribute to a better understanding of the role of cucumber CsLecRLK genes in response to PGPF, PPM, and PGPF+PPM treatments and lay the basis for the characterization of this important functional gene family.

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Section: Discussionsupporting

confidence: 92%

Genome-Wide Identification, Diversification, and Expression Analysis of Lectin Receptor-Like Kinase (LecRLK) Gene Family in Cucumber under Biotic Stress

Haider

Britto

Nagaraj

et al. 2021

IJMS

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“…Second, chitin treatment and Bgt treatment could both induce the transcriptional activation of LecRK-V. Furthermore, a G-type LecRLK from rice, Pi-d2, has been reported to contribute to plant defense against the fungal pathogen Magnaporthe grisea (Chen et al, 2006;Li et al, 2015). A single amino acid substitution at position 441 from Isoleucine (I) to Methionine (M) within its transmembrane domain could differentiate the resistant Pi-d2 allele from the susceptible ones.…”

Section: Lecrlks In Plant-microbe Pathogenic Interactionmentioning

confidence: 99%

Lectin Receptor-Like Kinases: The Sensor and Mediator at the Plant Cell Surface

et al. 2020

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Lectin receptor-like kinases (LecRLKs), a plant-specific receptor-like kinase (RLK) sub-family, have been recently found to play crucial roles in plant development and responses to abiotic and biotic stresses. In this review, we first describe the classification and structures of Lectin RLKs. Then we focus on the analysis of functions of LecRLKs in various biological processes and discuss the status of LecRLKs from the ligands they recognize, substrate they target, signaling pathways they are involved in, to the overall regulation of growth-defense tradeoffs. LecRLKs and the signaling components they interact with constitute recognition and protection systems at the plant cell surface contributing to the detection of environmental changes monitoring plant fitness.

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“…The non-synonymous mutations that occur in the CDS will lead to creating diversity at the protein level (Lu et al, 2012). Based on these non-synonymous mutations it is possible to identify functional protein-types owing to the fact that mutations at functional domains could lead to a conformational change of the protein resulting altered functionality (Lu et al, 2012;Wei et al, 2014;Li et al, 2015). In the current study, based on 14 such sites with nonsynonymous mutations (10 SNPs and 4 InDel sites) within the CDS, nine protein-types XA21-1 to XA21-9) were identified ( Figure 2).…”

Section: Thirteen Confirmed Xa21 Haplotypes and Nine Protein-typesmentioning

confidence: 99%

Haplotype diversity analysis of bacterial leaf blight resistance gene <i>Xa21</i> in rice

2018

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Bacterial leaf blight (BLB) caused by Xanthomonasoryzaepv. oryzae (Xoo) is a devastative rice disease. Resistance gene Xa21 is known to convey a stable resistance against a broad spectrum of Xoo races. Assessment of Xa21 genetic diversity and defining haplotypes is the initial step towards identification of haplotypes that associate with desired phenotypes. In this study, an alignment of a 2,778 bp coding sequence (CDS) from 1,618 rice accessions revealed 18 single nucleotide polymorphisms and six insertion/deletions (InDel), each with ≥ 5% occurrence among the accessions. A total of 13 confirmed-haplotypes (≥ 1% occurrence) and 113 putative haplotypes (≤ 1 % occurrence) were identified. The 13 confirmed haplotypes were represented by 1,341 rice accessions that deferred at 22 sites belonging to 12 rice subpopulations from 70 countries. The Xa21 CDS has a haplotype diversity of 0.8203, and a nucleotide diversity of 0.15448. Further, the haplotype network shows a high substitution rate between the haplotype pairs of Xa21, with two distinctive clusters created as a result of a 19-bp InDel in CDS. Based on 14 non-synonymous sites identified within the CDS, nine protein-types were identified. Of these three protein-types are truncated owing to a premature stop codon at 180 th position of the polypeptide chain due to the said 19-bp InDel in the ectodomain, and represents 45% of the accessions. The ectodomain of XA21 protein is crucial for immune responses, and hence, these haplotypes with truncated proteins could be important in eliciting the function of XA21.

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Natural variation of rice blast resistance gene Pi-d2

Cited by 21 publications

References 29 publications

Genome-Wide Identification, Diversification, and Expression Analysis of Lectin Receptor-Like Kinase (LecRLK) Gene Family in Cucumber under Biotic Stress

Genome-Wide Identification, Diversification, and Expression Analysis of Lectin Receptor-Like Kinase (LecRLK) Gene Family in Cucumber under Biotic Stress

Lectin Receptor-Like Kinases: The Sensor and Mediator at the Plant Cell Surface

Haplotype diversity analysis of bacterial leaf blight resistance gene <i>Xa21</i> in rice

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