Natural variation of carotenoids in the eggs and gonads of the echinoid genus, Strongylocentrotus: implications for their role in ultraviolet radiation photoprotection

Lamare, Miles D.; Hoffman, Jennifer R.

doi:10.1016/j.jembe.2004.02.016

Cited by 44 publications

(23 citation statements)

References 28 publications

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“…Mycosporine-like amino acids (MAAs), which absorb UVR wavelengths, have been found in many taxa including echinoid echinoderms (Shick & Dunlap 2002. Antioxidant carotenoids, which protect cells from reactive oxygen species (Edge et al 1997), reduce damage from UVR exposure in sea urchin embryos (Lamare & Hoffman 2004). Alternatively, many organisms are able to tolerate exposure to UVR because they are able to efficiently detect and repair cellular damage and DNA mutations (Sinha & Hader 2002, Hader & Sinha 2005.…”

Section: Discussionmentioning

confidence: 99%

Solar radiation plays a role in habitat selection by the sea star Pisaster ochraceus

Burnaford

Vasquez²

2008

Mar. Ecol. Prog. Ser.

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Section: Discussionmentioning

confidence: 99%

Solar radiation plays a role in habitat selection by the sea star Pisaster ochraceus

Burnaford

Vasquez²

2008

Mar. Ecol. Prog. Ser.

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“…However, to our knowledge there is only one published account of MAAs in the widely studied California purple sea urchin, S. purpuratus (Lamare and Hoffman 2004). In their study, Lamare and Hoffman (2004) found extremely low concentrations of MAAs in gonadal extracts of a single S.…”

Section: Introduction To the Manuscriptmentioning

confidence: 94%

“…The purple sea urchin stands out as a widelystudied model organism in molecular biology, and was the first invertebrate deuterostome to have its genome sequenced (Sodergren et al 2006). However, to our knowledge there is only one published account of MAAs in the widely studied California purple sea urchin, S. purpuratus (Lamare and Hoffman 2004). In their study, Lamare and Hoffman (2004) found extremely low concentrations of MAAs in gonadal extracts of a single S.…”

Section: Introduction To the Manuscriptmentioning

confidence: 99%

“…for ovaries and eggs, respectively), and spectrophotometric scans showed little light absorbance in the UV range (Lamare and Hoffman 2004;Sinha et al 2007). …”

mentioning

confidence: 99%

“…purpuratus was the first invertebrate deuterostome to have its genome sequenced (Sodergren et al 2006), and is important for structuring ecologically and economically valuable kelp forest and rocky intertidal ecosystems (Dayton 1975;Pearse 2006). It has long been a model organism for developmental biology (Jasny and Purnell 2006), including numerous studies on the effects of UV radiation on development (Wells and Giese 1950;Giese 1964;Zeitz et al 1968;Lamare and Hoffman 2004). Thus, an understanding of the presence, distribution and functions of MAAs in adult and larval S. purpuratus is imperative to understand the physiology of this model organism.…”

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confidence: 99%

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Sex and Microhabitat Influence the Allocation of Mycosporine-Like Amino Acids to Tissues in the Purple Sea Urchin, Strongylocentrotus Purpuratus

Gravem

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Improved Comet assay for the assessment of UV genotoxicity in Mediterranean sea urchin eggs

Nahon

Charles

Pruski

2008

Environ and Mol Mutagen

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Gametes and embryos of broadcast spawners are exposed to a wide range of chemical and physical stressors which may alone, or in conjunction, have serious consequences on reproductive outcomes. In this study, two Mediterranean echinoid species, Paracentrotus lividus and Sphaerechinus granularis, were chosen as models to study the genotoxicity of UV radiation (UVR) on the eggs of broadcast-spawning marine invertebrates. The single cell gel electrophoresis, or Comet assay, was successfully adapted to assess DNA strand breakage in sea urchin eggs. The results demonstrated that the genetic material of sea urchin eggs is susceptible to environmentally realistic UV exposure. The induction of DNA damage in the irradiated unfertilized eggs suggests that the previously described defense mechanisms in sea urchin eggs do not completely protect the egg's DNA against UV toxicity. Taken together, our results suggest that UV-impairment of the genetic integrity of the eggs might have a role in postfertilization failures and abnormal embryonic development. Although both species were vulnerable to UVR, embryonic development was less dramatically impaired in P.Lividus. This observation supports the postulation that species inhabiting shallower environments possess more efficient mechanisms to overcome UV-induced DNA alterations. The present demonstration of the utility and sensitivity of the Comet assay to evaluate DNA integrity in eggs from marine invertebrates opens new perspectives for monitoring the long-term effects of environmental exposure on populations and for the routine screening of substances for genotoxicity in marine systems.

show abstract

Natural variation of carotenoids in the eggs and gonads of the echinoid genus, Strongylocentrotus: implications for their role in ultraviolet radiation photoprotection

Cited by 44 publications

References 28 publications

Solar radiation plays a role in habitat selection by the sea star Pisaster ochraceus

Solar radiation plays a role in habitat selection by the sea star Pisaster ochraceus

Sex and Microhabitat Influence the Allocation of Mycosporine-Like Amino Acids to Tissues in the Purple Sea Urchin, Strongylocentrotus Purpuratus

Improved Comet assay for the assessment of UV genotoxicity in Mediterranean sea urchin eggs

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