Natural variation in the fast phase of chlorophyll a fluorescence induction curve (OJIP) in a global rice minicore panel

Khan, Naveed Ullah; Essemine, Jemâa; Hamdani, Saber; Qu, Mingnan; Lyu, Ming‐Ju Amy; Perveen, Shahnaz; Stirbet, Alexandrina; Govindjee, Govindjee

doi:10.1007/s11120-020-00794-z

Cited by 30 publications

(20 citation statements)

References 101 publications

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“…The chlorophyll fluorescence reflects the photochemical activity of PS II and the redox status of plastoquinone. Each stage in this transient reflects a particular phase in the electron transport system: The O-J phase in the transient is linked to QA reduction (Schreiber et al, 1987), and the rise from the J-I phase is due to the accumulation of QA- to QB- and subsequent QA - to QB and O to P raise in the fluorescence yield reflecting the variable fluorescence raise Fv is due to reduction of PQ pool size (Khan et al, 2021). The typical fluorescence transient curve of wild-type culture C-50 was shown in ( Figure 3A ); comparing it with the rest showed variation in their transient curve.…”

Section: Resultsmentioning

confidence: 99%

Low polyethylene glycol (PEG) concentration contributes to photoprotection in Chlamydomonas reinhardtii under highlight stress

Xavier

Yadav

Subramanyam

2022

Preprint

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The highlight is one of the major problems encountered by all the autotrophs in the natural environment, as it affects the photosynthetic performance of these organisms, they were equipped themself with the diverse photoprotective mechanism. However, the drastic climate change in recent years had a more lethal effect on these organisms as it is beyond their threshold to withstand. In the past decade, scientists have unravelled many photoprotective mechanisms like qE, qZ, qT and qI (NPQ) used by autotrophs to combat highlight stress and the thirst for discovering such a new mechanism remains constant. In this regard, we studied the effect of mild osmotic stress (2%PEG) in alleviating high-light stress using Chlamydomonas (C) reinhardtii as a model system. The cells were grown in low Polyethylene glycol (PEG)- induced osmotic stress at varying light intensities; their response to these treatments had been examined via biochemical and biophysical approaches. The PEG-treated cells showed better growth and photosynthetic efficiency even at highlight than control, but their NPQ level is lesser than control, suggesting a unique or novel photoprotective mechanism is operating in PEG-treated samples. The Circular dichroism and ATG8 localization assay suggest that supercomplexes organization is not much disturbed in PEG-treated samples irrespective of light intensity. Also, the latter indicates that PEG-treated cells were healthier than the control at the highlight. This result is promising as we can improve the algal biomass under natural environmental conditions with fluctuating light intensity. As algal biomass has immense commercial importance in biofuel production, cosmetics and pharmaceutical application. This mechanism can be exploited to promote the socio-economic status of our nation.

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Section: Resultsmentioning

confidence: 99%

Low polyethylene glycol (PEG) concentration contributes to photoprotection in Chlamydomonas reinhardtii under highlight stress

Xavier

Yadav

Subramanyam

2022

Preprint

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“…The OJIP transient curve obtained from Chl fluorescence experiments is extremely sensitive to environmental stressors (Calatayud and Barreno 2001, Sayed 2003, Van Heerden et al 2003, Govindachary et al 2004, Badr and Brüggemann 2020, Khan et al 2021. The shape of the OJIP transient changed over the first hours of hypoxia resulting in a significant decline in the variable fluorescence of all phases (Kumar et al 2020(Kumar et al , 2021Soni et al 2021, Bhatt et al 2022a.…”

Section: Effect Of Waterlogging On Psii and Chl Fluorescence Parametersmentioning

confidence: 99%

“…The shape of the OJIP transient changed over the first hours of hypoxia resulting in a significant decline in the variable fluorescence of all phases (Kumar et al 2020(Kumar et al , 2021Soni et al 2021, Bhatt et al 2022a. The fast O to J increase is the phase regulated by photochemical mechanisms, whereas J to I is entirely a thermal phase (Neubauer and Schreiber 1987, dos Santos Junior et al 2015, Khan et al 2021. The process that occurs on the donor site of the PSII regulates the release of fluorescence from this phase.…”

Section: Effect Of Waterlogging On Psii and Chl Fluorescence Parametersmentioning

confidence: 99%

Ultrastructure, adaptability, and alleviation mechanisms of photosynthetic apparatus in plants under waterlogging: A review

Sharma¹,

Bhatt²,

Sharma³

et al. 2022

Photosynt.

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“…One minute after light change, the state transition of photosystem and the activities of the Calvin-Benson cycle enzymes will also change [ 17 ]. The concentrations of metabolites involved in the photosynthetic metabolism respond to changes in light and CO 2 levels at a time scale of from 0.1 s to several minutes [ 21 – 23 ], which is much shorter than the response of gene expression (30 min and longer) or protein translation (10 h and longer) [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

An integrated isotopic labeling and freeze sampling apparatus (ILSA) to support sampling leaf metabolomics at a centi-second scale

Tang

Song

et al. 2022

Plant Methods

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Background Photosynthesis close interacts with respiration and nitrogen assimilation, which determine the photosynthetic efficiency of a leaf. Accurately quantifying the metabolic fluxes in photosynthesis, respiration and nitrogen assimilation benefit the design of photosynthetic efficiency improvement. To accurately estimate metabolic fluxes, time-series data including leaf metabolism and isotopic abundance changes should be collected under precisely controlled environments. But for isotopic labelled leaves under defined environments the, time cost of manually sampling usually longer than the turnover time of several intermediates in photosynthetic metabolism. In this case, the metabolic or physiological status of leaf sample would change during the sampling, and the accuracy of metabolomics data could be compromised. Results Here we developed an integrated isotopic labeling and freeze sampling apparatus (ILSA), which could finish freeze sampling automatically in 0.05 s. ILSA can not only be used for sampling of photosynthetic metabolism measurement, but also suit for leaf isotopic labeling experiments under controlled environments ([CO2] and light). Combined with HPLC–MS/MS as the metabolic measurement method, we demonstrated: (1) how pool-size of photosynthetic metabolites change in dark-accumulated rice leaf, and (2) variation in photosynthetic metabolic flux between rice and Arabidopsis thaliana. Conclusions The development of ILSA supports the photosynthetic research on metabolism and metabolic flux analysis and provides a new tool for the study of leaf physiology.

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Natural variation in the fast phase of chlorophyll a fluorescence induction curve (OJIP) in a global rice minicore panel

Cited by 30 publications

References 101 publications

Low polyethylene glycol (PEG) concentration contributes to photoprotection in Chlamydomonas reinhardtii under highlight stress

Low polyethylene glycol (PEG) concentration contributes to photoprotection in Chlamydomonas reinhardtii under highlight stress

Ultrastructure, adaptability, and alleviation mechanisms of photosynthetic apparatus in plants under waterlogging: A review

An integrated isotopic labeling and freeze sampling apparatus (ILSA) to support sampling leaf metabolomics at a centi-second scale

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