Natural variation for sulfate content in Arabidopsis thaliana is highly controlled by APR2

Loudet, Olivier; Saliba-Colombani, Véra; Camilleri, Christine; Calenge, Fanny; Gaudon, Virginie; Kopřivová, Anna; North, Kathryn A.; Kopriva, Stanislav; Daniel‐Vedele, Françoise

doi:10.1038/ng2050

Cited by 157 publications

(152 citation statements)

References 26 publications

Supporting

Mentioning

138

Contrasting

Unclassified

Order By: Relevance

“…Our study included the accessions Bay-0 and Sha, which have been extensively used in natural variation and QTL studies, among others for flowering time (Loudet et al, 2002), root architecture (Loudet et al, 2005), partial resistance to Pseudomonas syringae (Perchepied et al, 2006), phosphate starvation (Reymond et al, 2006;Svistoonoff et al, 2007), sulfate content (Loudet et al, 2007), shoot mineral content (Buescher et al, 2010), and growth on acidic soil (Poormohammad Kiani et al, 2012). As they cluster very closely in low K (Fig.…”

Section: Discussionmentioning

confidence: 99%

Natural Variation of Arabidopsis Root Architecture Reveals Complementing Adaptive Strategies to Potassium Starvation

2013

View full text Add to dashboard Cite

Root architecture is a highly plastic and environmentally responsive trait that enables plants to counteract nutrient scarcities with different foraging strategies. In potassium (K) deficiency (low K), seedlings of the Arabidopsis (Arabidopsis thaliana) reference accession Columbia (Col-0) show a strong reduction of lateral root elongation. To date, it is not clear whether this is a direct consequence of the lack of K as an osmoticum or a triggered response to maintain the growth of other organs under limiting conditions. In this study, we made use of natural variation within Arabidopsis to look for novel root architectural responses to low K. A comprehensive set of 14 differentially responding root parameters were quantified in K-starved and Kreplete plants. We identified a phenotypic gradient that links two extreme strategies of morphological adaptation to low K arising from a major tradeoff between main root (MR) and lateral root elongation. Accessions adopting strategy I (e.g. Col-0) maintained MR growth but compromised lateral root elongation, whereas strategy II genotypes (e.g. Catania-1) arrested MR elongation in favor of lateral branching. K resupply and histochemical staining resolved the temporal and spatial patterns of these responses. Quantitative trait locus analysis of K-dependent root architectures within a Col-0 3 Catania-1 recombinant inbred line population identified several loci each of which determined a particular subset of root architectural parameters. Our results indicate the existence of genomic hubs in the coordinated control of root growth in stress conditions and provide resources to facilitate the identification of the underlying genes.

show abstract

Section: Discussionmentioning

confidence: 99%

Natural Variation of Arabidopsis Root Architecture Reveals Complementing Adaptive Strategies to Potassium Starvation

2013

View full text Add to dashboard Cite

show abstract

“…Interestingly, the two genes shown to control variation in sulfate levels in Arabidopsis have not been found among the candidate genes. These two genes were shown to underlie QTLs from an analysis of a Bay-0 3 Shahdara population, and they encode isoforms of two consecutive enzymes of the sulfate assimilation pathway, ATP sulfurylase and APR (Loudet et al, 2007;Koprivova et al, 2013). The APR2 gene in Shahdara contained a unique SNP that resulted in an amino acid substitution near the active center and inactivation of the enzyme (Loudet et al, 2007).…”

Section: Power Of Atmentioning

confidence: 99%

“…These two genes were shown to underlie QTLs from an analysis of a Bay-0 3 Shahdara population, and they encode isoforms of two consecutive enzymes of the sulfate assimilation pathway, ATP sulfurylase and APR (Loudet et al, 2007;Koprivova et al, 2013). The APR2 gene in Shahdara contained a unique SNP that resulted in an amino acid substitution near the active center and inactivation of the enzyme (Loudet et al, 2007). ATP sulfurylase (ATPS) affects sulfate levels due to variation in transcript levels of the main isoform, ATPS1, caused by a deletion in intron 1 found in a few accessions (Koprivova et al, 2013).…”

Section: Power Of Atmentioning

confidence: 99%

“…These traits can be analyzed through quantitative trait locus (QTL) analysis (Loudet et al, 2003(Loudet et al, , 2007Harada et al, 2004;Reymond et al, 2006;Habash et al, 2007;Ding et al, 2010) or genome-wide association studies (GWAS; Atwell et al, 2010;Chan et al, 2011;Harper et al, 2012). The usefulness of GWAS has been demonstrated by capturing numerous well-characterized candidate genes (Aranzana et al, 2005;Atwell et al, 2010).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Dissection of the Control of Anion Homeostasis by Associative Transcriptomics in Brassica napus

et al. 2014

Self Cite

View full text Add to dashboard Cite

To assess the variation in nutrient homeostasis in oilseed rape and to identify the genes responsible for this variation, we determined foliar anion levels in a diversity panel of Brassica napus accessions, 84 of which had been genotyped previously using messenger RNA sequencing. We applied associative transcriptomics to identify sequence polymorphisms linked to variation in nitrate, phosphate, or sulfate in these accessions. The analysis identified several hundred significant associations for each anion. Using functional annotation of Arabidopsis (Arabidopsis thaliana) homologs and available microarray data, we identified 60 candidate genes for controlling variation in the anion contents. To verify that these genes function in the control of nutrient homeostasis, we obtained Arabidopsis transfer DNA insertion lines for these candidates and tested them for the accumulation of nitrate, phosphate, and sulfate. Fourteen lines differed significantly in levels of the corresponding anions. Several of these genes have been shown previously to affect the accumulation of the corresponding anions in Arabidopsis mutants. These results thus confirm the power of associative transcriptomics in dissection of the genetic control of complex traits and present a set of candidate genes for use in the improvement of efficiency of B. napus mineral nutrition.

show abstract

“…PAPS is also a precursor of 3Ј-phosphoadenosine 5Ј-phosphate, which is a critical metabolite for stress gene regulation and plant development (15). Control of sulfate flux between the primary (reductive) and secondary metabolic routes in plants helps to maintain the synthesis of the metabolites essential for plant growth and responses to different stresses (11)(12)(13)(14)(15)(16)(17)(18)(19).…”

mentioning

confidence: 99%

Nucleotide Binding Site Communication in Arabidopsis thaliana Adenosine 5′-Phosphosulfate Kinase

Ravilious

Jez

2012

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Adenosine 5Ј-phosphosulfate kinase (APSK) catalyzes the synthesis of phosphoadenosine 5Ј-phosphosulfate, but how APSK coordinates binding of phosphonucleotides is unclear. Results: Using calorimetry, crystallography, and mutagenesis, this study provides new insight on nucleotide binding in APSK. Conclusion:The P-loop and a critical aspartate integrate dynamic structural and nucleotide recognition features. Significance: These results suggest how structural changes guide the order of nucleotide addition for catalysis.

show abstract

Natural variation for sulfate content in Arabidopsis thaliana is highly controlled by APR2

Cited by 157 publications

References 26 publications

Natural Variation of Arabidopsis Root Architecture Reveals Complementing Adaptive Strategies to Potassium Starvation

Natural Variation of Arabidopsis Root Architecture Reveals Complementing Adaptive Strategies to Potassium Starvation

Dissection of the Control of Anion Homeostasis by Associative Transcriptomics in Brassica napus

Nucleotide Binding Site Communication in Arabidopsis thaliana Adenosine 5′-Phosphosulfate Kinase

Contact Info

Product

Resources

About