Natural variation analysis of perennial ryegrass in response to abiotic stress highlights LpHSFC1b as a positive regulator of heat stress

Sun, Tianxiao; Shao, Kun; Huang, Yan; Lei, Yuyang; Tan, Luyun; Chan, Zhulong

doi:10.1016/j.envexpbot.2020.104192

Cited by 26 publications

(18 citation statements)

References 58 publications

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“…Thermotolerance assay of transgenic Arabidopsis and WT was performed as described previously (Sun et al, 2020). The 14‐day‐old plants of transgenic Arabidopsis and WT were used for heat treatment at 45°C for 16 h. Leaves were collected 0, 6, and 12 h after 45°C treatment to measure electrolyte leakage (EL) and malondialdehyde (MDA) content.…”

Section: Methodsmentioning

confidence: 99%

Genome‐wide identification of heat shock transcription factor families in perennial ryegrass highlights the role of LpHSFC2b in heat stress response

Sun

Wang

et al. 2022

Physiologia Plantarum

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Perennial ryegrass (Lolium perenne) is a cool‐season turf and forage grass. Heat shock transcription factors (HSFs) play an important role in regulating plant abiotic stress. However, HSFs in perennial ryegrass have rarely been characterized. Here, 25 LpHSFs were identified from the perennial ryegrass genome. Phylogenetic analysis showed that the LpHSFs could be classified into 12 subclasses. Gene structure analysis showed that 22 LpHSFs have only one intron. Cis‐acting elements analysis revealed that the promoter of 15 LpHSFs contained hormone‐responsive and abiotic stress‐responsive elements. Expression profile analysis indicated that 24 LpHSFs were differentially expressed under submerge, drought, heat, and cold stresses. In addition, a subclass C2 gene, LpHSFC2b, was significantly induced by abiotic stresses. The LpHSFC2b protein is localized to the nucleus, and heterologous expression of LpHSFC2b in Arabidopsis improves plant thermotolerance. This study provides insights useful for the breeding of stress tolerance in perennial ryegrass.

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Section: Methodsmentioning

confidence: 99%

Genome‐wide identification of heat shock transcription factor families in perennial ryegrass highlights the role of LpHSFC2b in heat stress response

Sun

Wang

et al. 2022

Physiologia Plantarum

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“…ABA content was measured using an ELISA kit (H251, Jiancheng Bioengineering Institute, Nanjing, China) based on the protocol provided by the manufacturer. Soluble sugar content was determined using the 3,5-dinitrosalicylic acid method ( Sun et al, 2020 ). Leaf fresh weight was adopted in assessing these indicators above.…”

Section: Methodsmentioning

confidence: 99%

Functional Characterization of the Pheophytinase Gene, ZjPPH, From Zoysia japonica in Regulating Chlorophyll Degradation and Photosynthesis

et al. 2021

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Pheophytinase (PPH), the phytol hydrolase, plays important roles in chlorophyll degradation. Nevertheless, little attention has been paid to the PPHs in warm-season grass species; neither its detailed function in photosynthesis has been systematically explored to date. In this study, we isolated ZjPPH from Zoysia japonica, an excellent warm-season turfgrass species. Quantitative real-time PCR analysis and promoter activity characterization revealed that the expression of ZjPPH could be induced by senescence, ABA, and dark induction. Subcellular localization observation proved that ZjPPH was localized in the chloroplasts. Overexpression of ZjPPH accelerated the chlorophyll degradation and rescued the stay-green phenotype of the Arabidopsis pph mutant. Moreover, ZjPPH promoted senescence with the accumulation of ABA and soluble sugar contents, as well as the increased transcriptional level of SAG12 and SAG14. Transmission electron microscopy investigation revealed that ZjPPH caused the decomposition of chloroplasts ultrastructure in stable transformed Arabidopsis. Furthermore, chlorophyll a fluorescence transient measurement analysis suggested that ZjPPH suppressed photosynthesis efficiency by mainly suppressing both photosystem II (PSII) and photosystem I (PSI). In conclusion, ZjPPH plays an important role in chlorophyll degradation and senescence. It could be a valuable target for genetic editing to cultivate new germplasms with stay-green performance and improved photosynthetic efficiency.

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“…Heterologous expression of LpHSFC1b in Arabidopsis enhances the plants’ heat tolerance. The contents of electrolyte leakage (EL) and malondialdehyde (MDA) in Arabidopsis transformed with LpHSFC1b decreased, and the expression of the heat stress-responsive genes increased significantly [ 11 ]. Furthermore, HSFA1b is also a positive regulator of heat stress and the overexpression of HSFA1b increased heat tolerance in Arabidopsis [ 88 , 89 , 90 ].…”

Section: Heat Stressmentioning

confidence: 99%

“…Perennial ryegrass ( Lolium perenne L.), native to Europe, Asia, and Northern Africa, is a cool-season perennial grass cultivated around the world with a breeding history of more than 100 years [ 11 , 12 ]. Due to its outstanding forage quality, long growing season, high yield, grazing tolerance, and high palatability, perennial ryegrass is the most widely cultivated perennial gramineous forage in temperate regions [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Insights into the Response of Perennial Ryegrass to Abiotic Stress: Underlying Survival Strategies and Adaptation Mechanisms

Miao

Zhang

Bai

et al. 2022

Life

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Perennial ryegrass (Lolium perenne L.) is an important turfgrass and gramineous forage widely grown in temperate regions around the world. However, its perennial nature leads to the inevitable exposure of perennial ryegrass to various environmental stresses on a seasonal basis and from year to year. Like other plants, perennial ryegrass has evolved sophisticated mechanisms to make appropriate adjustments in growth and development in order to adapt to the stress environment at both the physiological and molecular levels. A thorough understanding of the mechanisms of perennial ryegrass response to abiotic stresses is crucial for obtaining superior stress-tolerant varieties through molecular breeding. Over the past decades, studies of perennial ryegrass at the molecular and genetic levels have revealed a lot of useful information to understand the mechanisms of perennial ryegrass adaptation to an adverse environment. Unfortunately, molecular mechanisms by which perennial ryegrass adapts to abiotic stresses have not been reviewed thus far. In this review, we summarize the recent works on the genetic and molecular mechanisms of perennial ryegrass response to the major abiotic stresses (i.e., drought, salinity, and extreme temperatures) and discuss new directions for future studies. Such knowledge will provide valuable information for molecular breeding in perennial ryegrass to improve stress resistance and promote the sustainability of agriculture and the environment.

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Natural variation analysis of perennial ryegrass in response to abiotic stress highlights LpHSFC1b as a positive regulator of heat stress

Cited by 26 publications

References 58 publications

Genome‐wide identification of heat shock transcription factor families in perennial ryegrass highlights the role of LpHSFC2b in heat stress response

Genome‐wide identification of heat shock transcription factor families in perennial ryegrass highlights the role of LpHSFC2b in heat stress response

Functional Characterization of the Pheophytinase Gene, ZjPPH, From Zoysia japonica in Regulating Chlorophyll Degradation and Photosynthesis

Insights into the Response of Perennial Ryegrass to Abiotic Stress: Underlying Survival Strategies and Adaptation Mechanisms

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