Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil

Silva, Maelin da; Matoso, Daniele Aparecida; Ludwig, Luis Augusto M.; Gomes, Eder; Almeida, Mara Cristina de; Vicari, Marcelo Ricardo; Artoni, Roberto Ferreira

doi:10.1007/s10641-011-9794-2

Cited by 13 publications

(6 citation statements)

References 26 publications

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“…For the same species, Maistro et al (1994) detected various frequencies of natural triploids, such as 1/86 and 1/40. However, Da Silva et al (2011) detected increased numbers of triploid individuals in Rhamdia quelen under natural conditions. As natural triploidy can vary with species and the local sampling environment (Tsuda et al , 2010), we believe that the absence of triploidy in the other treatments could be due the random collection process, which means that if more samples were analyzed, more triploid fish would be obsereved.…”

Section: Discussionmentioning

confidence: 98%

In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae

Nascimento

Lázaro

Alcântara

et al. 2018

Zygote

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SummaryIn this study we analyzed whether the in vivo storage of oocytes (time after ovulation until fertilization) affects the survival and the ploidy status of the yellowtail tetra Astyanax altiparanae. Fish were induced to spawn and, after ovulation, a small aliquot was stripped and immediately fertilized (positive control group). Subsequently, aliquots (~150 oocytes) were stripped and fertilized at various time points of 60, 120, 180 or 240 min. Developmental stages, abnormalities, survival and the ploidy status of the hatched larvae were examined. As expected, in the control group, 100% of the larvae were diploid. Conversely, triploid individuals were observed just at the 60 min treatment time point (0.6%). In vivo storage of oocytes also influenced the survival rates (P < 0.05); the 180 and 240 min samples, respectively, presented lower survival rates at gastrula (50.10±6.26% and 40.92±5.32%), and somite (17.80±5.14% and 4.41±2.76%) stages and lower hatching rates (12.01±4.04% and 4.41±2.76%). A higher percentage (99.27±0.40%) of normal larvae and only a few abnormal larvae (0.73±0.40%) were observed in the control group (P = 0.0000). This observation did not differ from that observed at the 60 min treatment point (P = 0.9976). A significant increase in the percentage of abnormalities was observed in the other treatments, and, after 240 min, the highest percentage of abnormal larvae was seen (P=0.0024; 83.33±16.67%). In conclusion, we showed that oocyte ageing had a significant effect on survival and may affect the ploidy status in A. atiparanae.

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Section: Discussionmentioning

confidence: 98%

In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae

Nascimento

Lázaro

Alcântara

et al. 2018

Zygote

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“…[31][32][33][34][35][36] In aquaculture, fish triploid induction for Neotropical species have been developed for jundiá Rhamdia quelen. [37][38][39] In these experiments, the protocols allow obtaining 100% of triploids. 20,40 Therefore, jundia triploids could be used on a commercial scale because of the difficulties for largescale production of diploids, such as early sexual maturation and heterogeneity in growth rate.…”

Section: Discussionmentioning

confidence: 99%

Triploidy in tambaqui Colossoma macropomum identified by chromosomes of fish larvae

Hashimoto¹

2020

JAMB

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Triploid fish are excellent models for use in commercial scale because they can show higher rates of growth, carcass yield and meat quality. If sterile, tambaqui (Colosso mamacropomum) triploids can be used to avoid the problems related to early puberty, especially the negative impacts of male sexual maturation on harvest weight. In the present study, we evaluated the triploidy induction efficiency (thermal shocks) in the fish tambaqui Colossoma macropomum, the most important Neotropical species used for aquaculture. To verify the result of the triploidy experiments, we used a protocol of ploidy identification through analysis of mitotic chromosomes of early life stages (larvae cytogenetics). We obtained better results of hatching and triploid rates through temperature shock at 41°C for 2 min (heat shock) than cold shock. In conclusion, additional treatments will allow to refine the protocols to obtain 100% of triploidy in tambaqui, particularly those from heat shocks. These methodologies can also be applied to other Neotropical fish species, as alternative methods for breeding programs and to delineate conservation plans for the proper management of the stocks.

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“…Studies that characterize the chromosomal locations of 5S and 18S rDNA in Siluriformes are scarce ( Kavalco et al 2004 ; Centofante et al 2006 ; Mendes-Neto et al 2011). A few such studies were conducted in Pimelodidade and Pseudopimelodidae , where non-syntenic 5S and 18S ribosomal regions were observed ( Carvalho and Dias 2007 ; Garcia and Moreira-Filho 2008 ; Marques et al 2008 ; Matoso et al 2011 ; Moraes Neto et al 2011 ; Silva et al 2011 ). In Loricariidae , chromosomes with syntenic 5S and 18S regions were observed in some groups like Neoplecostominae and the out group Trichomycteridae ( Ziemniczak 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal diversification in ribosomal DNA sites in Ancistrus Kner, 1854 (Loricariidae, Ancistrini) from three hydrographic basins of Mato Grosso, Brazil

Mariotto¹,

Centofante²,

Vicari³

et al. 2011

CCG

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Populations of seven Ancistrus species were analyzed from streams and rivers of three hydrographic Brazilian basins. All populations showed different diploid numbers (2n), fundamental numbers (FNs), and karyotypes. Some representatives of Loricariidae have 2n = 54 chromosomes, which is very likely an ancestral cytotaxonomic characteristic, but many other representatives show extensive karyotype diversification. In the Ancistrus species studied, extensive karyotypic differentiation, which is generally associated with chromosome number reduction and rearrangement of the ribosomal RNA gene (rDNA) sites, was verified. Chromosomal locations of 18S and 5S rDNA were jointly detected using fluorescence in situ hybridization (FISH). In all the Ancistrus species analyzed, 18S rDNA sites were detected only on one chromosome pair, though this differed among species. 5S rDNA was located on 1–3 chromosome pairs either separately or in synteny with 18S rDNA in four of the seven species/populations. Hence the karyotype differentiation in Ancistrus species could be associated with a morphological speciation process, suggesting that chromosome fusions, inversions, deletions, duplications, and heterochromatination could contribute to the karyotype evolution of these neotropical armored catfishes.

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Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil

Cited by 13 publications

References 26 publications

In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae

In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae

Triploidy in tambaqui Colossoma macropomum identified by chromosomes of fish larvae

Chromosomal diversification in ribosomal DNA sites in Ancistrus Kner, 1854 (Loricariidae, Ancistrini) from three hydrographic basins of Mato Grosso, Brazil

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