Natural selenium particles from Staphylococcus carnosus: Hazards or particles with particular promise?

Estevam, Ethiene Castellucci; Griffin, Sharoon; Nasim, Muhammad Jawad; Denezhkin, Polina; Schneider, Ramona; Lilischkis, Rainer; Domínguez‐Álvarez, Enrique; Witek, Karolina; Latacz, Gniewomir; Keck, Cornelia M.; Schäfer, Karl‐Herbert; Kieć‐Kononowicz, Katarzyna; Handzlik, Jadwiga; Jacob, Claus

doi:10.1016/j.jhazmat.2016.02.001

Cited by 53 publications

(44 citation statements)

References 26 publications

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“…Indeed, in our own hands, the nanoparticles obtained for Cynomorium, and here in particular for the outer layer, were significantly more active against C. albicans when compared to nanoparticles of the chalcogens sulphur, selenium and tellurium, as well as particles of S. incanum and P. erinaceus, whose formation and activity we have reported previously [6,15,20,21].…”

Section: Discussionsupporting

confidence: 70%

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Nanosizing Cynomorium: Thumbs up for Potential Antifungal Applications

et al. 2017

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Cynomorium coccineum L., the desert thumb, is a rather exotic, parasitic plant unable to engage in photosynthesis, yet rich in a variety of unique compounds with a wide spectrum of biological applications. Whilst extraction, separation and isolation of such compounds is time consuming, the particular properties of the plant, such as dryness, hardness and lack of chlorophyll, render it a prime target for possible nanosizing. The entire plant, the external layer (coat) as well as its peel, are readily milled and high pressure homogenized to yield small, mostly uniform spherical particles with diameters in the range of 300 to 600 nm. The best quality of particles is obtained for the processed entire plant. Based on initial screens for biological activity, it seems that these particles are particularly active against the pathogenic fungus Candida albicans, whilst no activity could be observed against the model nematode Steinernema feltiae. This activity is particularly pronounced in the case of the external layer, whilst the peeled part does not seem to inhibit growth of C. albicans. Thanks to the ease of sample preparation, the good quality of the nanosuspension obtained, and the interesting activity of this natural product, nanosized coats of Cynomorium may well provide a lead for future development and applications as "green" materials in the field of medicine, but also environmentally, for instance in agriculture.

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Section: Discussionsupporting

confidence: 70%

“…As guided by the literature, microbial growth assays were performed monitoring the optical density in order to obtain appropriate growth curves [14,15]. The optical density was measured at 540 nm with a Varian Cary 50 Bio UV-Visible spectrophotometer (Varian Australia Pty Ltd., Mulgrave, Australia).…”

Section: Antimicrobial Analysismentioning

confidence: 99%

Nanosizing Cynomorium: Thumbs up for Potential Antifungal Applications

et al. 2017

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“…Remarkably, against E. coli and S. aureus, nSe exhibited higher inhibitory effect in comparison to the standard antibiotic ampicillin plausibly due to the small size of nSe that can permeate the membrane and induce cell lysis. It has been reported that metallic nanoparticles interfere with the respiratory sequence and transport protein synthesis, terminate cell division and induce microbial cell death [53,57,58]. Overall, the results clearly indicate that the nSe posesses broad spectrum antimicrobial activity towards both Gram positive and negative bacterial strains.…”

Section: Micro Broth Dilution Assay: Minimum Inhibitory Concentrationmentioning

confidence: 70%

“…Raman spectral analysis was in corroboration with the XRD and SAED results. Next, the zeta potential of aqueous solution of nSe was determined and was found to be highly negative (18.1±0.65 mV), which did not change with time and therefore correlates with the stability of the particles for several weeks ( figure S5(a)) [46,53]. Moreover, DLS studies predicted an average hydrodynamic size of nSe around 164±6.4 nm ( figure S5(b)).…”

Section: Resultsmentioning

confidence: 96%

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

et al. 2020

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The exploitation of plant extracts for the synthesis of nano selenium having antibacterial and antioxidant activities is an exciting approach to counteract the prevalence of infections caused by antibiotic-resistant bacteria, which holds relevance for medical and food industries. In the present work, a green and facile method for the preparation of nano selenium (nSe) using the fruit extract of Indian gooseberry (Phyllanthus Emblica) has been reported. The optical and structural properties of the as-synthesized nSe were studied through various characterization techniques. Eventually, the antioxidant potential of nSe was investigated via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl free radical scavenging assays. Parallely, the antibacterial activity of nSe against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa was evaluated. The antioxidant assays indicated that even low dosage of nSe showed excellent activity with EC 50 values of 0.21 μg ml −1 and 3.34 μg ml −1 , respectively. Moreover, nSe exhibited significant inhibition in bacterial growth at low minimum inhibitory concentration (MIC) values against Escherichia coli (16 μg ml −1 ), Staphylococcus aureus (32 μg ml −1 ) and Pseudomonas aeruginosa (48 μg ml −1 ) compared to MIC values for standard drug ampicillin. Importantly, nSe did not induce any cytotoxic effects on normal human keratinocytes (HaCaT) at the tested concentrations; representing their biocompatible nature. The data obtained demonstrated the versatility of phytogreen nSe as a potent antioxidant and antibacterial agent to effectively prevent as well as treat multidrug-resistant bacterial infections.

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“…Indeed, such a concept may be useful to cover: (a) newly identified natural selenium metabolites, such as selenoneine, a rather unusual natural selenium compound which has been found in the blood of tuna and seems to be highly reactive, (b) inorganic selenium species with biological relevance, such as naturally generated selenium nanoparticles found in many strains of Staphylococcus and other bacteria and used on occasion to fortify drinks with selenium, (c) selenium agents currently in development with potential pharmaceutical applications, such as organic seleninic acids, organic selenourea derivatives, selenazolinium salts, and (d) recently discovered selenoproteins and the various modifications of selenocysteine found already or to be expected in those proteins (such as selenosulfide, diselenide, seleninic acids, selenoxides, etc.) [126,127,128,129,130,131,132,133,134,135,136,137,138,139,140,141,142]. Whilst those RSeS at first sight may be less relevant to redox biology compared to RSS because of their number, variety and concentration in most cells and organisms, they are clearly on the rise in the field of food supplementation and pharmaceutical research.…”

Section: Discussionmentioning

confidence: 99%

The Reactive Sulfur Species Concept: 15 Years On

et al. 2017

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Fifteen years ago, in 2001, the concept of “Reactive Sulfur Species” or RSS was advocated as a working hypothesis. Since then various organic as well as inorganic RSS have attracted considerable interest and stimulated many new and often unexpected avenues in research and product development. During this time, it has become apparent that molecules with sulfur-containing functional groups are not just the passive “victims” of oxidative stress or simple conveyors of signals in cells, but can also be stressors in their own right, with pivotal roles in cellular function and homeostasis. Many “exotic” sulfur-based compounds, often of natural origin, have entered the fray in the context of nutrition, ageing, chemoprevention and therapy. In parallel, the field of inorganic RSS has come to the forefront of research, with short-lived yet metabolically important intermediates, such as various sulfur-nitrogen species and polysulfides (Sx2−), playing important roles. Between 2003 and 2005 several breath-taking discoveries emerged characterising unusual sulfur redox states in biology, and since then the truly unique role of sulfur-dependent redox systems has become apparent. Following these discoveries, over the last decade a “hunt” and, more recently, mining for such modifications has begun—and still continues—often in conjunction with new, innovative and complex labelling and analytical methods to capture the (entire) sulfur “redoxome”. A key distinction for RSS is that, unlike oxygen or nitrogen, sulfur not only forms a plethora of specific reactive species, but sulfur also targets itself, as sulfur containing molecules, i.e., peptides, proteins and enzymes, preferentially react with RSS. Not surprisingly, today this sulfur-centred redox signalling and control inside the living cell is a burning issue, which has moved on from the predominantly thiol/disulfide biochemistry of the past to a complex labyrinth of interacting signalling and control pathways which involve various sulfur oxidation states, sulfur species and reactions. RSS are omnipresent and, in some instances, are even considered as the true bearers of redox control, perhaps being more important than the Reactive Oxygen Species (ROS) or Reactive Nitrogen Species (RNS) which for decades have dominated the redox field. In other(s) words, in 2017, sulfur redox is “on the rise”, and the idea of RSS resonates throughout the Life Sciences. Still, the RSS story isn’t over yet. Many RSS are at the heart of “mistaken identities” which urgently require clarification and may even provide the foundations for further scientific revolutions in the years to come. In light of these developments, it is therefore the perfect time to revisit the original hypotheses, to select highlights in the field and to question and eventually update our concept of “Reactive Sulfur Species”.

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Natural selenium particles from Staphylococcus carnosus: Hazards or particles with particular promise?

Cited by 53 publications

References 26 publications

Nanosizing Cynomorium: Thumbs up for Potential Antifungal Applications

Nanosizing Cynomorium: Thumbs up for Potential Antifungal Applications

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

The Reactive Sulfur Species Concept: 15 Years On

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