. 2001. Three-component barley mixtures: Ratio effects in replacement series. Can. J. Plant. Sci. 81: 651-656. Within a species, cultivar mixtures may offer yield and quality advantages if the cultivars have complementary abiotic and biotic stress tolerances. This study was conducted at Botha, Lacombe and Olds, Alberta, from 1992 to 1994 to determine the effect of relative seeding ratios on yield and other traits of 16 three-component barley (Hordeum vulgare L.) mixtures of Virden:Abee:Tukwa all grown at a standard seeding rate of 250 seeds m -2 . Grain yields of these mixtures fell between the yields of the monocrops, with yields of the 20:40:40 and 50:30:20 mixtures being higher than expected based on the weighted mean yields of the monocrops. When stability of yield was measured using ranking or regression analyses, several mixtures had desirable combinations of high yields and good stability with the 20:40:40 and the 40:20:40 mixtures being identified using either method. Test weights, kernel weights, percent thins, protein contents, and disease levels of the mixtures were intermediate to the monocrops; while lodging levels were as low as the best monocrop. As the proportion of any one cultivar in the mixture increased, the traits it brought to the mixture also increased. These mixtures had no yield advantage over growing a high yielding monocrop. Le poids spécifique, le poids d'amande, le pourcentage de graines minces, la concentration de protéines et le taux de maladie des mélanges se situent à mi-chemin de la valeur correspondante pour la culture pure, mais le taux de verse était égal au plus faible enregistré pour la monoculture. Quand la proportion de graines d'un cultivar augmente dans le mélange, les caractères de ce dernier se démarquent aussi davantage. Les mélanges ne présentent aucun avantage sur la production d'une seule espèce à rendement élevé.