Natural product isolation – how to get from biological material to pure compounds

Bučar, Franz; Wube, Abraham Abebe; Schmid, Martin G.

doi:10.1039/c3np20106f

Cited by 293 publications

(173 citation statements)

References 234 publications

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“…A previous report using GC-MS showed the presence of hydrocarbons in the seed oil of D. regia (Adewuyi et al 2010). Working at elevated temperatures to obtain the essential oil could lead to chemical changes, e.g., essential oils of chamomile (blue chamazulene originating from colorless matricin) (Bucar et al 2013). Chamazulene was found in the present study, but in small amounts (1.75%).…”

Section: Antifungal Activity Assaycontrasting

confidence: 38%

Stem Wood and Bark Extracts of Delonix regia (Boj. Ex. Hook): Chemical Analysis and Antibacterial, Antifungal, and Antioxidant Properties

Salem¹,

Abdel-Megeed²,

Ali³

2014

BioResources

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In the present study, the fatty acid components of the wood, bark, and essential oil of wood from Delonix regia as well as its antibacterial, antifungal, and antioxidant properties were investigated for the potential ability to control plant and human pathogens. Myristic acid was found to be a major fatty acid in the wood and bark of Delonix regia, comprising 10.77% of wood and 9.63% of bark. According to the GC-MS results, naphthalene derivatives were detected in the essential oils from the wood samples. Heptadecane and acyclic hydrocarbons were found in a high percentage (14.05%). Methanol: chloroform (1:1 v/v) wood extract showed effective activity against Bacillus subtilis, Sarcina lutea, and Staphylococcus aureus, where the bark extract was most active against Escherichia coli. The essential oil showed good antibacterial activity against Pectobacterium carotovorum. The bark extract showed the maximum percentage inhibition of fungal mycelial growth against Penicillium selerotigenum (70.37%) and Paecilomyces variotii (77.78%), and the essential oil showed moderate inhibition against Aspergillus nigra (44.44%). The total antioxidant activity of essential oil, stem wood, and stem bark extract was 84.34%, 80.33%, and 70.21%, respectively.

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Section: Antifungal Activity Assaycontrasting

confidence: 38%

Stem Wood and Bark Extracts of Delonix regia (Boj. Ex. Hook): Chemical Analysis and Antibacterial, Antifungal, and Antioxidant Properties

Salem¹,

Abdel-Megeed²,

Ali³

2014

BioResources

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“…The classical solvent extraction of polyphenols usually includes extraction by maceration and percolation and by successive Soxhlet extraction [41][42][43][44][45].…”

Section: Classical Solvent Extractionmentioning

confidence: 99%

Phenolics in Foods: Extraction, Analysis and Measurements

Aires¹

2017

Phenolic Compounds - Natural Sources, Importance and Applications

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The increasing consumers demands to acquire healthier fruits and vegetables as well as the urgency in looking to natural compounds with antioxidant activity and enhanced antimicrobial activity against antibiotic-resistant pathogenic bacterial strains have encouraged a quick expansion of research studies about enhanced phenolic extraction and identiication methods. Considering the importance of phenolics as natural compounds with antioxidant and antimicrobial activity, this chapter aims to present the most updated information about extraction methods, ranging from the traditional to the most advanced processes, as well as the access to the modern methods used in the identiication and quantiication of phenolics. The main goal of this chapter is to provide the reader with a broad view on the diferent protocols used to extract, identify and quantify phenolic compounds from diferent kinds of foods, including fruits and vegetables.Keywords: phytochemicals, extraction, determination, colorimetric methods, HPLC, HPLC-MS IntroductionThe growing resistance of pathogenic bacterial isolates against the traditional chemical antibiotics as well as the resurgent of old disappeared diseases associated with the constant consumers' demanding of healthier, nutritious and safe food has led the researchers to focus on searching for new, safe and efective molecules. One class of such molecules is the class of polyphenols. Polyphenols are a ubiquitous class of compounds largely present in plants as their secondary metabolites, which are synthesized during their normal development [1] in response to several stressful biotic and abiotic factors [2,3]. This class of compounds are a much diversiied group derived from the amino acids phenylalanine and tyrosine and The results from the last decade's research have shown that polyphenols have important beneicial properties for human health, including antioxidative, antiaging, antibacterial and antimutagenic [7][8][9][10][11]. Moreover, the recent evidence of their interaction with proteins, DNA and other biological molecules has enhanced their exploitation for the production of new natural product-derived therapeutic agents. Despite these advantages, several limitations still persist, particularly those related with their extraction eiciency, which afects the large-scale use of some of these substances. The diiculties in screening, extracting, separation and purifying these compounds have increased the development of new and modern methods to address these limitations. In this context, the aim of this chapter is to present an updated review about sources, technologies and methods that have been developed until now to improve the extraction, detection, separation and full characterization of such beneicial compounds, with special emphasis to their possible application in the design of nutraceuticals and functional food products. Foods as natural resources of phenolicsPolyphenols have been exhaustively studied in their diferent natural matrices such as fruits, vegetables, teas, algae a...

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“…Examples of preparative separations have been performed for synthetic products, bioactive molecules extracted from biological sources, and for racemates of chiral drugs (Francotte, 2009;Sticher, 2008;Bucar et al, 2013). Typical analytical separations have been applied for the quantification of compounds in complex mixtures, the determination of enantiomeric excess of scalemic mixtures, and the identification of organic compounds by hyphenated chromatographic techniques (Wilkins, 1983;Holt et al, 1997;Ellis and Roberts, 1997;Guetens et al, 2002a,b).…”

Section: Introductionmentioning

confidence: 99%