Natural product corynoline suppresses melanoma cell growth through inducing oxidative stress

Yi, Chunyang; Li, Xiaolong; Chen, Si; Liu, Mingyao; Lü, Weiqiang; Ye, Xiyun

doi:10.1002/ptr.6719

Cited by 12 publications

(5 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although several new drugs have been developed over the last 10 years that have greatly improved the prognosis of patients with metastatic melanoma, many patients do not show a lasting response to these treatments [ 5 ]. Treatment with natural compounds and preparations based on plant materials have been increasingly used among patients with various neoplasms [ 6 , 7 , 8 ], and natural product-based treatments for melanoma are constantly being researched [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Anticancer Potential of Jasione montana and Its Main Components against Human Amelanotic Melanoma Cells

Juszczak

Czarnomysy

Strawa

et al. 2021

IJMS

View full text Add to dashboard Cite

Jasione montana L. (Campanulaceae) is used in traditional Belarusian herbal medicine for sleep disorders in children, but the chemical composition and biological activity have not been investigated. In this study, the activities of J. montana extracts, their fractions and main compounds were evaluated in amelanotic melanoma C32 (CRL-1585) cells and normal fibroblasts (PCS-201-012). The extracts and fractions were analyzed using liquid chromatography–photodiode array detection–electrospray ionization–mass spectrometry (LC–PDA–ESI–MS/TOF) to characterize 25 compounds. Further, three major and known constituents, luteolin (22) and its derivatives such as 7-O-glucoside (12) and 7-O-sambubioside (9) were isolated and identified. The cytotoxic activities against fibroblasts and the amelanotic melanoma cell line were determined using the fixable viability stain (FVS) assay. The influence of diethyl ether (Et2O) fraction (JM4) and 22 on apoptosis induction was investigated using an annexin V binding assay. The obtained results showed significant cytotoxicity of JM4 and 22 with IC50 values of 119.7 ± 3.2 and 95.1 ± 7.2 μg/mL, respectively. The proapoptotic potential after 22 treatment in the C32 human amelanotic melanoma cell line was comparable to that of vinblastine sulfate (VLB), detecting 29.2 ± 3.0% apoptotic cells. Moreover, 22 displayed less necrotic potential against melanoma cells than VLB. In addition, the influences of JM4 and 22 on the dysfunction of the mitochondrial membrane potential (MMP), cell cycle and activity of caspases 3, 8, 9, and 10 were established. The effects of JM4 on MMP change (74.5 ± 3.0% of the cells showed a reduced MMP) corresponded to the results obtained from the annexin V binding assay and activation of caspase-9. JM4 and 22 displayed a significant impact on caspase-9 (40.9 ± 2.4% of the cells contained active caspase-9 after JM4 treatment and 16.6 ± 0.8% after incubation with 22) and the intrinsic (mitochondrial) apoptotic pathway. Moreover, studies have shown that JM4 and 22 affect the activation of external apoptosis pathways by inducing the caspase-8 and caspase-10 cascades. Thus, activation of caspase-3 and DNA damage via external and internal apoptotic pathways were observed after treatment with JM4 and 22. The obtained results suggest that J. montana extracts could be developed as new topical preparations with potential anticancer properties due to their promising cytotoxic and proapoptotic potential.

show abstract

Section: Introductionmentioning

confidence: 99%

In Vitro Anticancer Potential of Jasione montana and Its Main Components against Human Amelanotic Melanoma Cells

Juszczak

Czarnomysy

Strawa

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Some therapeutic options already exist for melanoma, but their effectiveness is still limited, mainly due to acquired resistance [ 55 ]. Thus, the investigation of propolis activity in this type of cancer is important due to the need for new treatments and sources of therapeutic agents [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Phenolic Compounds Contribution to Portuguese Propolis Anti-Melanoma Activity

et al. 2023

View full text Add to dashboard Cite

Melanoma is the deadliest type of skin cancer, with about 61,000 deaths annually worldwide. Late diagnosis increases mortality rates due to melanoma’s capacity to metastasise rapidly and patients’ resistance to the available conventional therapies. Consequently, the interest in natural products as a strategy for drug discovery has been emerging. Propolis, a natural product produced by bees, has several biological properties, including anticancer effects. Propolis from Gerês is one of the most studied Portuguese propolis. Our group has previously demonstrated that an ethanol extract of Gerês propolis collected in 2018 (G18.EE) and its fractions (n-hexane, ethyl acetate, and n-butanol) decrease melanoma cell viability. Out of all the fractions, G18.EE-n-BuOH showed the highest potential as a melanoma pharmacological therapy. Thus, in this work, G18.EE-n-BuOH was fractioned into 17 subfractions whose effect was evaluated in A375 BRAF-mutated melanoma cells. The subfractions with the highest cytotoxic activity were analysed by UPLC-DAD-ESI/MSn in an attempt to understand which phenolic compounds could account for the anti-melanoma activity. The compounds identified are typical of the Gerês propolis, and some of them have already been linked with antitumor effectiveness. These results reaffirm that propolis compounds can be a source of new drugs and the isolation of compounds could allow its use in traditional medicine.

show abstract

“…Acetyldihydrosanguinarine (24), 6-ketenesanguinarine (25), and demethylsanguinarine (26) (Figure 8) are isolated from Corydalis bungeana Turcz. Xiyun Ye et al [62] determined the cytotoxic activity of the above two compounds against A549, HT-29, kb16, and P-388 cell lines, respectively, and their ED50 (µg/mL) values reach 1.840, 1.600, 0.340, and 0.051, respectively. Havelek R. et al [61] have indicated that homochelidonine (23) (Figure 8) induces apoptosis and arrests the G2 phase mitotic cell cycle in cancer cells, and 20 µM homochelidonine (23) inhibits the cell growth of SK-BR-3, HepG2, and MCF-7 by over 50%.…”

Section: Antitumor Activities Of Benzophenanthridine Alkaloids From C...mentioning

confidence: 99%

Section: Antitumor Activities Of Benzophenanthridine Alkaloids From C...mentioning

confidence: 99%

“…Acetylcorynoline (29) potently inhibits human colon carcinoma HCT-116, Acetyldihydrosanguinarine (24), 6-ketenesanguinarine (25), and demethylsanguinarine (26) (Figure 8) are isolated from Corydalis bungeana Turcz. Xiyun Ye et al [62] determined the cytotoxic activity of the above two compounds against A549, HT-29, kb16, and P-388 cell lines, respectively, and their ED 50 (µg/mL) values reach 1.840, 1.600, 0.340, and 0.051, respectively. Corygaline A (28) (Figure 9), isolated from Corydalis bungeana Turcz, refers to a hexahydrobenzophenanthridine alkaloid with an unusual carbon skeleton.…”

Section: Antitumor Activities Of Benzophenanthridine Alkaloids From C...mentioning

confidence: 99%

See 1 more Smart Citation

Insights on Antitumor Activity and Mechanism of Natural Benzophenanthridine Alkaloids

Peng,

Xu,

Xie

et al. 2023

Molecules

View full text Add to dashboard Cite

Benzophenanthridine alkaloids are a class of isoquinoline compounds, which are widely found in the plants of papaveraceae, corydalis, and rutaceae. Biological activities and clinical studies have shown that benzophenanthridine alkaloids have inhibitory effects on many cancers. Considering that the anticancer activities and mechanisms of many natural benzophenanthridine alkaloids have been discovered in succession, the purpose of this paper is to review the anticancer effects of benzophenanthridine alkaloids and explore the application potential of these natural products in the development of antitumor drugs. A literature survey was carried out using Scopus, Pubmed, Reaxys, and Google Scholar databases. This review summarizes and analyzes the current status of research on the antitumor activity and antitumor mechanism of natural products of benzophenanthridine from different sources. The research progress of the antitumor activity of natural products of benzophenanthridine from 1983 to 2023 was reviewed. The antitumor activities of 90 natural products of benzophenanthridine and their related analogues were summarized, and the results directly or indirectly showed that natural products of benzophenanthridine had the effects of antidrug-resistant tumor cell lines, antitumor stem cells, and inducing ferroptosis. In conclusion, benzophenanthridine alkaloids have inhibitory effects on a variety of cancers and have the potential to counteract tumor resistance, and they have great application potential in the development of antitumor drugs.

show abstract

Natural product corynoline suppresses melanoma cell growth through inducing oxidative stress

Cited by 12 publications

References 59 publications

In Vitro Anticancer Potential of Jasione montana and Its Main Components against Human Amelanotic Melanoma Cells

In Vitro Anticancer Potential of Jasione montana and Its Main Components against Human Amelanotic Melanoma Cells

Phenolic Compounds Contribution to Portuguese Propolis Anti-Melanoma Activity

Insights on Antitumor Activity and Mechanism of Natural Benzophenanthridine Alkaloids

Contact Info

Product

Resources

About