Natural Populations of Galphimia spp. Attenuates In Vitro and In Vivo Inflammatory Responses

Gesto-Borroto, Reinier; Meneses, Gabriela; Espinosa-Cerón, Alejandro; Granados, Guillermo; Cervantes‐Torres, Jacquelynne; Cardoso-Taketa, Alexandre; Sciutto, Edda; Villarreal, Marı́a Luisa

doi:10.1007/s43450-022-00325-y

Cited by 2 publications

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The anti-in ammatory potential of the methanolic extract of Galphimia spp. from Morelos population was con rmed by inhibition of nitrites production in the murine macrophages cell line RAW 264.7 (Gesto-Borroto et al, 2022). Nor-triterpenes galphimidin (1) and galphimidin B (2) were isolated from the population of Morelos (Camacho et al, 2002;Rios et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory, Radical-Scavenging, and Chelating Activities of Nor-Triterpenes from Galphimia Species

León-Álvarez,

Pacheco,

Gesto-Borroto

et al. 2024

Rev. Bras. Farmacogn.

Self Cite

View full text Add to dashboard Cite

Anti-in ammatory and radical-scavenging activities of the methanolic extract from the leaves of Galphimia spp. collected in Morelos, Mexico, and isolated galphimidin (1) and galphimidin B (2), were evaluated. Nor-triterpenes 1 and 2 displayed anti-in ammatory activity inhibiting the nitric oxide production by the LPS-induced RAW 264.7 cell model, with IC 50 values of 22.62 and 20.19 µg/mL, respectively, which were higher than the crude extract with an IC 50 of 42.92 µg/mL. Furthermore, the TPA (12-O-tetradecanoylphorbol-13-acetate)-induced ear edema in mice assay displayed results of EC 50 of 2.03 mg/ear to the methanolic extract, 1.91 mg/ear to 1, and 1.42 mg/ear to 2. Docking analysis of 1 and 2 at the iNOS active site indicated the negative in uence of water molecules by mediating the ligand-protein interaction through hydrogen bonds interactions that increase the energy expenditure. These results explain the observed anti-in ammatory action of 1 and 2 by iNOS inhibition, which is better modulated in silico by the absence of water in the active site.The methanolic extract showed a low radical-scavenging activity inhibiting free radicals DPPH • with values of 57.33% compared to isoquercetin and rutin as positive controls. Nevertheless, the crude extract displayed high activity inhibiting ABTS •+ free radicals at 83.31% that was in accordance with high reducing potential of total polyphenols by Folin-Ciocalteu method with value of 3591.11 mg of gallic acid (GAE)/g of biomass. Compounds 1 and 2 did not present radical-scavenging activities but showed metalchelating activity of 27.62% and 30.54%, which were higher values than EDTA as positive control.

show abstract

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory, Radical-Scavenging, and Chelating Activities of Nor-Triterpenes from Galphimia Species

León-Álvarez,

Pacheco,

Gesto-Borroto

et al. 2024

Rev. Bras. Farmacogn.

Self Cite

View full text Add to dashboard Cite

show abstract

Anti-inflammatory, Radical-Scavenging, and Chelating Activities of Nor-triterpenes from Galphimia spp. (Malpighiaceae)

León-Álvarez,

Pacheco,

Gesto-Borroto

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Anti-inflammatory and radical-scavenging activities of the methanolic extract from the leaves of Galphimia spp. collected in Morelos, Mexico, and isolated galphimidin (1) and galphimidin B (2), were evaluated. Nor-triterpenes 1 and 2 displayed anti-inflammatory activity inhibiting the nitric oxide production by the LPS-induced RAW 264.7 cell model, with IC50 values of 22.62 and 20.19 µg/mL, respectively, which were higher than the crude extract with an IC50 of 42.92 µg/mL. Furthermore, the TPA (12-O-tetradecanoylphorbol-13-acetate)-induced ear edema in mice assay displayed results of EC50 of 2.03 mg/ear to the methanolic extract, 1.91 mg/ear to 1, and 1.42 mg/ear to 2. Docking analysis of 1 and 2 at the iNOS active site indicated the negative influence of water molecules by mediating the ligand-protein interaction through hydrogen bonds interactions that increase the energy expenditure. These results explain the observed anti-inflammatory action of 1 and 2 by iNOS inhibition, which is better modulated in silico by the absence of water in the active site. The methanolic extract showed a low radical-scavenging activity inhibiting free radicals DPPH• with values of 57.33% compared to isoquercetin and rutin as positive controls. Nevertheless, the crude extract displayed high activity inhibiting ABTS•+ free radicals at 83.31% that was in accordance with high reducing potential of total polyphenols by Folin-Ciocalteu method with value of 3591.11 mg of gallic acid (GAE)/g of biomass. Compounds 1 and 2 did not present radical-scavenging activities but showed metal-chelating activity of 27.62% and 30.54%, which were higher values than EDTA as positive control.

show abstract

Natural Populations of Galphimia spp. Attenuates In Vitro and In Vivo Inflammatory Responses

Cited by 2 publications

References 24 publications

Anti-inflammatory, Radical-Scavenging, and Chelating Activities of Nor-Triterpenes from Galphimia Species

Anti-inflammatory, Radical-Scavenging, and Chelating Activities of Nor-Triterpenes from Galphimia Species

Anti-inflammatory, Radical-Scavenging, and Chelating Activities of Nor-triterpenes from Galphimia spp. (Malpighiaceae)

Contact Info

Product

Resources

About