Natural Polymer–Based Micronanostructured Scaffolds for Bone Tissue Engineering

Katebifar, Sara; Jaiswal, Devina; Arul, Michael R.; Novak, Sanja; Nip, Jonathan; Kalajzić, Ivo; Rudraiah, Swetha; Kumbar, Sangamesh G.

doi:10.1007/978-1-0716-1811-0_35

Cited by 4 publications

(6 citation statements)

References 45 publications

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“…These structural characteristics provide a theoretical basis for the subsequent coculture and controlled release of peptides with DCs. Due to their adhesion, 37 biocompatibility, 38 and biodegradation, 39,40 peptides have been widely used in cell culture, 41 tissue engineering, 42,43 and local delivery of various drugs/genes. 44,45 Our experiments showed that RLDI and RQDT can continuously and stably release antigens, which provides a reliable basis for continuous and stable T-cell stimulation to produce specific immune responses when DC vaccines are injected into mice.…”

Section: Discussionmentioning

confidence: 99%

Engineering Self-Assembling Peptide Hydrogel to Enhance the Capacity of Dendritic Cells to Activate In Vivo T-Cell Immunity

Luo,

Wan,

Liu

et al. 2024

Biomacromolecules

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The efficacy of the dendritic cell (DC) has failed to meet expectations thus far, and crucial problems such as the immature state of DCs, low targeting efficiency, insufficient number of dendritic cells, and microenvironment are still the current focus.To address these problems, we developed two self-assembling peptides, RLDI and RQDT, that mimic extracellular matrix (ECM). These peptides can be self-assembled into highly ordered three-dimensional nanofiber scaffold structures, where RLDI can form gelation immediately. In addition, we found that RLDI and RQDT enhance the biological function of DCs, including releasing antigens sustainably, adhering to DCs, promoting the maturation of DCs, and increasing the ability of DC antigen presentation. Moreover, peptide hydrogel-based DC treatment significantly achieved prophylactic and treatment effects on colon cancer. These results have certain implications for the design of new broad-spectrum vaccines in the future.

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Section: Discussionmentioning

confidence: 99%

Engineering Self-Assembling Peptide Hydrogel to Enhance the Capacity of Dendritic Cells to Activate In Vivo T-Cell Immunity

Luo,

Wan,

Liu

et al. 2024

Biomacromolecules

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“…Currently, there is a need to develop BTE scaffolds to repair large bone defects that can overcome the limitations related to autografts, allografts, and xenografts without sacrificing the required mechanical strength and create body‐friendly degradation by‐products 39‐41 . In addition, the mechanical integrity should be maintained during the bone healing process 42 .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the quantitative analysis revealed a higher level of BSP expression on the composite scaffolds with TCP and BMP2 with no significant difference compared with the neat PCL and autograft positive controls (Figure 13B) Currently, there is a need to develop BTE scaffolds to repair large bone defects that can overcome the limitations related to autografts, allografts, and xenografts without sacrificing the required mechanical strength and create body-friendly degradation by-products. [39][40][41] In addition, the mechanical integrity should be maintained during the bone healing process. 42 In the past, scaffolding systems have been constructed using synthetic polymers, but because most of them have predefined pores, their mechanical properties are inferior to their bulk properties.…”

Section: Histological Analysis Of New Bone Formation In Rat Mandibula...mentioning

confidence: 99%

Novel high‐strength polyester composite scaffolds for bone regeneration

Katebifar,

Arul,

Abdulmalik

et al. 2023

Polymers for Advanced Techs

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Repair of critical‐sized bone defects, particularly in load‐bearing areas, is a major clinical problem that requires surgical intervention and implantation of biological or engineered grafts. For load‐bearing sites, it is essential to use engineered grafts that have both sufficient mechanical strength and appropriate pore properties to support bone repair and tissue regeneration. Unfortunately, the mechanical properties of such grafts are often compromised due to the creation of pores required to facilitate tissue ingrowth following implantation. To overcome the limitations associated with porous scaffolds and their reduced mechanical strength, we have developed a methodology for creating a solid structure that retains its bulk mechanical properties while also evolving into a porous structure in a biological environment through degradation and erosion. In this study, we utilized polyesters that have been approved by the FDA, including poly (lactic acid) (PLA), poly (glycolic acid) (PGA), their copolymer PLGA (PLGA, with a ratio of 85:15 and 50:50 of PLA:PGA), and poly (caprolactone) (PCL). These polymers and their ceramic composites with tricalcium phosphate (TCP) were compression molded into solid forms, which exhibited mechanical properties with compressive modulus as high as 2745 ± 364 MPa within the range of human trabecular bone and in the lower range of human cortical bone. The use of fast‐degrading PLGA (50:50) and PGA as porogens allowed the formation of pores within the solid structures due to their degradation, and the TCP acts as a buffering agent to neutralize their acidic degradation byproducts. These scaffolds facilitated the growth of new blood vessels and tissue ingrowth in a subcutaneous implantation model. In addition, in a rat critical‐sized mandibular bone defects these scaffolds supported bone growth with 70% of new bone volume fraction. Furthermore, the extent of bone regeneration was found to be higher for the scaffolds with bone morphogenic proteins (BMP2), indicating their suitability for bone repair and regeneration.

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“…The physical properties of synthetic biopolymers can be more precisely controlled. 26 However, both natural and synthetic biopolymers are often characterized by a lack of load-bearing mechanical properties. 27 Moreover, natural biopolymers may contain pathological impurities such as endotoxins, and most synthetic biopolymers lack cell-specific identification sites and are hydrophobic.…”

Section: Introductionmentioning

confidence: 99%

“…For biopolymers, an extracellular matrix can be used to create natural biopolymers with high biocompatibility and minimal toxicity. The physical properties of synthetic biopolymers can be more precisely controlled . However, both natural and synthetic biopolymers are often characterized by a lack of load-bearing mechanical properties .…”

Section: Introductionmentioning

confidence: 99%

Regulation of Immune Inflammation and Promotion of Periodontal Bone Regeneration by Irisin-Loaded Bioactive Glass Nanoparticles

Hu,

Lu,

Duan

et al. 2024

Langmuir

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Clinical solutions of bone defects caused by periodontitis involve surgical treatment and subsequent antiinfection treatment using antibiotics. Such a strategy faces a key challenge in that the excessive host immune response results in the damage of periodontal tissues. Consequently, it is of great importance to develop novel periodontitis treatment that allows the regulation of the host immune response and promotes the generation of periodontal tissues. Irisin has a good bone regeneration ability and could reduce the inflammatory reaction by regulating the differentiation of macrophages. In this study, we loaded irisin onto bioactive glass nanoparticles (BGNs) to prepare a composite, irisin-BGNs (IR-BGNs) with anti-inflammatory, bacteriostatic, and tissue regeneration functions, providing a novel idea for the design of ideal materials for repairing oral tissue defects caused by periodontitis. We also verified that the IR-BGNs had better anti-inflammatory properties on RAW264.7 cells compared to irisin and BGNs alone. Strikingly, when hPDLCs were stimulated with IR-BGNs, they exhibited increased expression of markers linked to osteogenesis, ALP activity, and mineralization ability in comparison to the negative control. Furthermore, on the basis of RNA sequencing results, we validated that the p38 pathway can contribute to the osteogenic differentiation of the IR-BGNs. This work may offer new thoughts on the design of ideal materials for repairing oral tissue defects.

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Natural Polymer–Based Micronanostructured Scaffolds for Bone Tissue Engineering

Cited by 4 publications

References 45 publications

Engineering Self-Assembling Peptide Hydrogel to Enhance the Capacity of Dendritic Cells to Activate In Vivo T-Cell Immunity

Engineering Self-Assembling Peptide Hydrogel to Enhance the Capacity of Dendritic Cells to Activate In Vivo T-Cell Immunity

Novel high‐strength polyester composite scaffolds for bone regeneration

Regulation of Immune Inflammation and Promotion of Periodontal Bone Regeneration by Irisin-Loaded Bioactive Glass Nanoparticles

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