Natural Piperine Improves Lipid Metabolic Profile of High-Fat Diet-Fed Mice by Upregulating SR-B1 and ABCG8 Transporters

Abstract

Natural piperine from black pepper is known to function as hypocholesterolemic agent, but how it lowers the blood cholesterol remains unclear. In this study, we found that intragastric administrations of piperine (25 mg/kg/day) for 8 weeks significantly reduced the plasma triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) in high-fat diet (HFD)-fed mice. H&E staining indicated that piperine significantly decreased hepatic lipid accumulation compared with the control group. The Oil Red O staining further showed that piperine attenuated lipid deposition in liver HepG2 cells in a concentration-dependent manner. Mechanistically, piperine treatment caused a significant upregulation of hepatic scavenger receptor B1 (SR-B1) in the liver and transporter protein of ATP binding cassette SGM8 (ABCG8) in the small intestine. Taken together, our findings demonstrate the role of natural piperine in improving lipid metabolic profile that is involved in the reverse cholesterol transport (RCT)-mediated mechanism through upregulation of SR-B1 in the liver and ABCG8 in the small intestine.

Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells

“…As presented in Figure 4 C–F, OA elevated cellular TG, TC, and LDL-C contents and reduced the level of HDL-C compared to the control group, suggesting that OA disrupted the lipid metabolism homeostasis in HepG2 cells. It was reported that PIP could regulate lipid levels by enhancing cholesterol efflux, reducing cholesterol uptake, and leading to a reversal of hepatic homeostasis induced by HFD [ 24 ]. In our experiment, after PIP treatment, TG, TC, and LDL-C contents were reduced dose-dependent, showing that PIP ameliorated the accumulation of lipids in HepG2 cells.…”

Structurally Various Sorbicillinoids From an Endophytic Fungus Acremonium citrinum SS-g13

“…cubense , Cucumber fusarium wilt , Cowpea wilt , F. graminearum , Nectria sp., F. mangiferae , C. asianum, and Alternaria solani ., using a disk diffusion and half dilution method ( Zhang et al, 2019 ). Effect of know compounds 6 , 7 , 8 , 11 , and 12 on serum-mediated cholesterol efflux and on cell viability in J774A.1 macrophages were also tested ( Sankaranarayanan et al, 2011 ; Wang et al, 2017 ; Hou et al, 2021 ).…”

Evodiamine Lowers Blood Lipids by Up-Regulating the PPARγ/ABCG1 Pathway in High-Fat-Diet-Fed Mice

“…Establishment of the Hyperlipidemic Model and Evodiamine Treatment. Mice were fed with a normal diet or a high-fat diet following the recipes 24 consisting of 3% cholesterol, 10% lard, 0.5% bile salt (pig) (Table 2). After 3 weeks of HFD, mouse blood was collected from the tail vein and plasma lipid levels were determined for verifying the establishment of a hyperlipidemic model.…”

“…After embedding in histoplast paraffin, the liver tissues were cut into 5 μm sections using a coolclamp tissue microtome (Leica, RM2255, Germany). Then the above sections were stained using modified H&E. 24 Images of sections were captured by a microscope (Nikon, Eclipse TS100, Japan).…”

“…In addition, we demonstrate that evodiamine inhibits the HFDinduced hepatic lipid accumulation as evident in H&E staining under the same experimental conditions described in our previous study. 24 For elimination, cholesterol needs to be converted to bile acids and expelled from hepatocytes to keep homeostasis and prevent hypercholesterolemia. 25 As expected, evodiamine also decreases TBA levels, which is consistent with reduced TC and TG levels in liver cells of the HFD group.…”