Natural oxidase-mimicking copper-organic frameworks for targeted identification of ascorbate in sensitive sweat sensing

Abstract

Sweat sensors play a significant role in personalized healthcare by dynamically monitoring biochemical markers to detect individual physiological status. The specific response to the target biomolecules usually depends on natural oxidase, but it is susceptible to external interference. In this work, we report tryptophan- and histidine-treated copper metal-organic frameworks (Cu-MOFs). This amino-functionalized copper-organic framework shows highly selective activity for ascorbate oxidation and can serve as an efficient ascorbate oxidase-mimicking material in sensitive sweat sensors. Experiments and calculation results elucidate that the introduced tryptophan/histidine fundamentally regulates the adsorption behaviors of biomolecules, enabling ascorbate to be selectively captured from complex sweat and further efficiently electrooxidized. This work provides not only a paradigm for specifically sweat sensing but also a significant understanding of natural oxidase-inspired MOF nanoenzymes for sensing technologies and beyond.

Artificial enzyme innovations in electrochemical devices: advancing wearable and portable sensing technologies

“…Its adeptness in replicating natural enzymatic processes, synergized with enhanced selectivity via chemical enhancements, paves the path for highly specific and bespoke health monitoring applications. 127 The innovative use of MOFs in sensor technology, as highlighted in the preceding examples, primarily centers around their function in catalyzing reactions and enhancing sensor sensitivity. However, the versatility of MOFs extends beyond these traditional roles.…”

“…Its adeptness in replicating natural enzymatic processes, synergized with enhanced selectivity via chemical enhancements, paves the path for highly specific and bespoke health monitoring applications. 127…”

Valence-Engineered Oxidase-Mimicking Nanozyme with Specificity for Aromatic Amine Oxidation and Identification

“…Nanozymes, a type of nanomaterials with enzyme-like activities, − have exhibited various applications in biosensing, , therapeutics, − and imaging fields. − Due to the tunable catalytic activities, high stability, low cost, recyclability, and robustness to harsh environments, nanozymes are becoming promising alternatives to natural enzymes. − Up to now, nanozymes exhibiting oxidase-like activity universally use 3,3′,5,5′-tetramethylbenzidine (TMB), 2,2-azinobis (3-ethylbenzothizoline-6-sulfonic acid) (ABTS), o -phenylenediamine (OPD), and 2,4-dichlorophenol (2,4-DP) as substrates to evaluate catalytic performance. As reported, oxidase-like nanozymes could simultaneously catalyze the oxidation of TMB, ABTS, OPD, and 2,4-DP. − Therefore, the specificity was still a great challenge. − …”

UiO-66 framework with an encapsulated spin probe: synthesis and exceptional sensitivity to mechanical pressure

“…The chemistry and applications of metal-organic frameworks (MOFs) have progressed significantly since their original inception more than two decades ago. [1][2][3][4][5][6][7] A huge amount of research has been focused on these compounds due to the virtually unlimited possibilities of adjusting their properties by changing the metal centres or organic linkers. [8][9][10][11][12][13][14] Variation of structures in MOFs allows obtaining compounds with different characteristics, such as average pore diameters, total pore volume and number of defects.…”