Natural occurrence of mycotoxins and mycoflora of argentinien popcorn

Pacin, A.; Boca, R. T.; González, Horacio F.; Resnik, Silvia L.; Burak, R.; Broccoli, Andrea; Souza, Jéssica

doi:10.1007/bf02946701

Cited by 7 publications

(5 citation statements)

References 8 publications

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“…The data on dominant fungal species on maize grains in Serbia are similar to those previously described worldwide (Abdel-Mallek et al, 1993;Mubatanhema et al, 1999;Hussein et al, 2002;Pacin et al, 2002;Logrieco et al, 2003;Somda et al, 2008;Ramos et al, 2010). However, when comparing the data on the incidence of mycobiota on maize seeds obtained and presented in this study with previously published results by Lević et al (1997) it can be deduced that the incidence of F. graminearum, F. oxysporum, F. subglutinans and F. verticillioides has changed over last 15 years.…”

Section: Discussionsupporting

confidence: 83%

Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia

et al. 2012

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SUMMARyA total of 41 species of fungi were isolated from seed samples of barley, maize, soybean, and sunflower collected at different locations in Serbia. The majority of detected species occurred on barley (35 of 41 species or 87.8%) comparing to soybean (17 of 41 species or 41.5%), sunflower (16 of 41 species or 39.0%) and maize (15 of 41 species or 36.9%). Species belonging to genera Alternaria, Chaetomium, Epicoccum, Fusarium, Penicillium and Rhizopus were present on seeds of all four plant species. Alternaria species were dominant on soybean, barley and sunflower seeds (85.7%, 84.7% and 76.9%). F. verticillioides and Penicillium spp. were mainly isolated from maize seeds (100 and 92.3% respectively), while other species were isolated up to 38.5% (Chaetomium spp. and Rhizopus spp.). F. graminearum, F. proliferatum, F. poae and F. sporotrichioides were the most common Fusarium species isolated from barley (51.1-93.3%), while on the soybean seeds F. oxysporum (71.4%), F. semitectum (57.1%) and F. sporotrichioides (57.1%) were prevalent. Frequency of Fusarium species on sunflower seeds varied from 7% (F. equiseti, F. graminearum, F. proliferatum and F. subglutinans) to 15.4% (F. verticillioides). Statistically significant negative correlation (r = -0.678*) was determined for the incidence of F. graminearum and Alternaria spp., as well as, Fusarium spp. and Alternaria spp. (r = -0.614*), on barley seeds. The obtained results revealed that seedborne pathogens were present in most seed samples of important cereals and industrial crops grown under different agroecological conditions in Serbia. Some of the identified fungi are potential producers of mycotoxins, thus their presence is important in terms of reduced food safety for humans and animals. Therefore, an early and accurate diagnosis and pathogen surveillance will provide time for the development and the application of disease strategies.

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Section: Discussionsupporting

confidence: 83%

Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia

et al. 2012

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“…The absence of DON in popcorn has already been mentioned in Portugal in 2008 by the analysis of 49 samples by TLC (Martins et al, 2008). Similar results were obtained by Pacin et al (2002) in Argentina through the analysis of 42 popcorn samples. However, in Brazil, a study with 105 popcorn samples reported DON in four samples with levels ranging from 32 to 40 mg/kg (Oliveira et al, 2001).…”

Section: Analysis Of Mycotoxins In Unpopped and Popped Popcornsupporting

confidence: 88%

“…The levels of LOQ are lower than the maximum residue limits established by EU for these mycotoxins (Commission Regulation N 1126/2007. Furthermore, the LOQ of DON was better than those obtained by Oliveira, Soares, and Sawazaki (2001) 150 mg/kg, Pacin et al (2002) 125 mg/kg and Martins, Almeida, Marques, and Guerra (2008) 100 mg/kg. Better LOQ were also obtained for ZON when compared to those obtained by Pacin et al (2002) 125 mg/kg.…”

Section: Limit Of Detection (Lod) and Limit Of Quantification (Loq)contrasting

confidence: 49%

Optimization and validation of a method based in a QuEChERS procedure and gas chromatography–mass spectrometry for the determination of multi-mycotoxins in popcorn

Ferreira¹,

Fernandes²,

Cunha³

2012

Food Control

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“…Of the two species identified here, F. verticillioides was consistently found both on the seed surface and within the internal tissues (Figure a). This species is the most frequently isolated species from maize seeds in other regions of world, for example, Argentina (Pacin et al., ), Mexico (Leyva‐Madrigal et al., ), and Serbia (Lević et al., ), and systematically infects all parts of plants (Oren et al., ). Isolates of F. verticillioides have also been demonstrated to be highly aggressive and capable of co‐infecting with other species of Fusarium (Leyva‐Madrigal et al., ).…”

Section: Discussionmentioning

confidence: 99%

Mycobiota of maize seeds revealed by rDNA‐ITS sequence analysis of samples with varying storage times

Hui-qin

Ma²,

et al. 2018

MicrobiologyOpen

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Fungi are an integral component of the plant microbiome. However, the composition and variation in the fungal communities (mycobiota) associated with seeds are poorly understood. In this study, we investigated the mycobiota of 11 maize seed samples with storage times ranging from 6 months to 12 years. Mycobiota were characterized by a culture-based approach, and fungal species were identified through rDNA-ITS sequence analyses. From a total of 169 pure fungal isolates obtained from both the seed surface and internal tissues, we identified 16 distinct species (belonging to 10 genera) associated with maize seeds, all but one of which were ascomycetes. Among these species, seven were exclusively isolated from internal tissues, two species were isolated only from the seed surface, and another six species were isolated from both the surface and internal tissues. Aspergillus niger was consistently found under all storage conditions and dominated fungal communities with a relative abundance of 36%-100%. Species of Fusarium (9%-40%) and Penicillium (9%-20%) were also frequently isolated, but other species appeared sporadically and were isolated from fewer than three seed stocks. According to our results, while the overall incidence of fungal infection generally declined with storage time, there was no consistent association between seed storage time and fungal species richness or relative abundance; furthermore, the composition of the mycobiota associated with maize seeds was highly variable among the samples. The detection of the four major mycotoxigenic fungal genera, specifically Aspergillus, Fusarium, Penicillium, and Alternaria, was alarming, and the isolation of a potential controlling agent as well as information about their temporal occurrence will contribute to the management of mycotoxins in the future.

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Natural occurrence of mycotoxins and mycoflora of argentinien popcorn

Cited by 7 publications

References 8 publications

Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia

Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia

Optimization and validation of a method based in a QuEChERS procedure and gas chromatography–mass spectrometry for the determination of multi-mycotoxins in popcorn

Mycobiota of maize seeds revealed by rDNA‐ITS sequence analysis of samples with varying storage times

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