Natural occurrence of aflatoxin in green leafy vegetables

Hariprasad, P.; Durivadivel, P.; Snigdha, M.; Venkateswaran, G.

doi:10.1016/j.foodchem.2012.11.093

Cited by 24 publications

(18 citation statements)

References 20 publications

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“…There are several reports showing the uptake of mycotoxins by various plants and their transport to aerial parts (Mertz et al, 1980(Mertz et al, , 1981Rao et al, 1982;Mantle, 2000;Zitomer et al, 2010;Hariprasad et al, 2013). Similarly, here we checked whether AFB 1 can be absorbed by roots of groundnut plants through several in vitro studies.…”

Section: Discussionmentioning

confidence: 90%

“…Uptake of aflatoxin by maize (Mertz et al, 1980(Mertz et al, , 1981, citrinin, patulin and terreic acid by rice seedlings (Rao et al, 1982), ochratoxin A by coffee plants (Mantle, 2000), and fumonisin by wheat plants (Zitomer et al, 2010) were the major studies where mycotoxin uptake was successfully proved. Moreover, earlier work conducted in our laboratory revealed the aflatoxins uptake ability of green leafy vegetables (Hariprasad et al, 2013).…”

Section: Introductionmentioning

confidence: 77%

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Transport via xylem and accumulation of aflatoxin in seeds of groundnut plant

2015

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 77%

Transport via xylem and accumulation of aflatoxin in seeds of groundnut plant

2015

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“…Total aflatoxins (AF) were estimated according to Reddy et al , with minor modifications . Standard curves using absorbance (A) versus logarithm of analyte concentration were plotted.…”

Section: Methodsmentioning

confidence: 99%

Analysis of genetic and aflatoxin diversity among Aspergillus flavus isolates collected from sorghum seeds

Divakara

Aiyaz

Moore

et al. 2015

J. Basic Microbiol.

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Thirty-four Aspergillus flavus isolates were recovered from sorghum seeds sampled across five states in India. Our study included (1) species confirmation through PCR assay, (2) quantification of total aflatoxin concentrations by the indirect competitive-ELISA (ic-ELISA) method, and (3) analysis of molecular diversity among the A. flavus isolates using β-tubulin, ITS, and ISSR markers. Among the isolates studied, 28 were found to be positive for the production of aflatoxins. ITS and β-tubulin phylogenetic analysis segregated the A. flavus sample population into two major groups or clades with little to no subdivision based on geography. In contrast, ISSR analysis also separated the A. flavus isolates into two main clusters, showing a distance of 0.0-0.5, with one cluster exhibiting a high level of diversity though no geographic or chemotype subdivision could be observed. The majority of sampled A. flavus isolates were highly toxigenic, and also highly diversified in terms of toxin-producing potential in-vitro. Genetic diversity among the sorghum isolates of A. flavus further warrants the development of appropriate farming management practices as well as improved aflatoxin detection measures in India.

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“…Total AF content was estimated from culture filtrate, soils and groundnut seeds by modified method of Reddy et al [27], and Hariprasad et al [28]. AFB 1 -BSA conjugate was prepared following the standard procedure [29].…”

Section: Indirect Competitive Enzyme Linked Immunosorbent Assay (Icelmentioning

confidence: 99%

Characterization of non-aflatoxigenic strains of Aspergillus flavus as potential biocontrol agent for the management of aflatoxin contamination in groundnut

Mallikarjunaiah

Jayapala

Hariprasad

et al. 2017

Microbial Pathogenesis

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In the present study, nine non-aflatoxigenic Aspergillus flavus strains were evaluated for their potential to reduce A. flavus infection and aflatoxin contamination in groundnut. Genetic characterization of these strains revealed six different deletion patterns (A-F) for thirteen examined genes from the aflatoxin biosynthesis pathway. Strain AFGS5 recorded maximum number of gene deletion (F) which included 12 out of 13 tested genes. Our findings indicated that aflR was the most frequently absent gene among the observed deletion patterns. A dendrogram inferred from combining random amplified polymorphic DNA and microsatellite data showed three of the non-aflatoxigenic strains segregating from other sampled isolates (aflatoxigenic and non-aflatoxigenic) tested. Greenhouse experiments, involving non-aflatoxigenic strains as biocontrol agents resulted in two strains, AFGS5 and AFGS12, which significantly reduced the population of aflatoxigenic fungi and the level of total aflatoxins in the rhizosphere/geocarposphere of soil samples as well as in groundnut seeds. Based on our findings, the use of these native non-aflatoxigenic strains; AFGS5 or AFGS12 in particular, as biopesticides could offer efficacious mitigation of aflatoxin contamination.

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Natural occurrence of aflatoxin in green leafy vegetables

Cited by 24 publications

References 20 publications

Transport via xylem and accumulation of aflatoxin in seeds of groundnut plant

Transport via xylem and accumulation of aflatoxin in seeds of groundnut plant

Analysis of genetic and aflatoxin diversity among Aspergillus flavus isolates collected from sorghum seeds

Characterization of non-aflatoxigenic strains of Aspergillus flavus as potential biocontrol agent for the management of aflatoxin contamination in groundnut

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