Natural limonoids protect mice from alcohol-induced liver injury

Valansa, Abacuc; Galani, Borris Rosnay Tietcheu; Chuisseu, Pascal Dieudonne Djamen; Tsamo, Armelle Tontsa; Owona, Vincent Brice Ayissi; Njintang, Nicolas Yanou

doi:10.1515/jbcpp-2019-0271

Cited by 7 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the pathogenesis of NAFLD has not yet been fully elucidated, and the determination of therapeutic targets and the advancement of drug development are severely limited. Current research shows that limonin has a wide range of pharmacological effects, including anti-cancer, anti-inflammation, anti-bacterial, anti-viral, anti-oxidation and liver protection properties ( Fan et al, 2019 ), and has been proven to have the effect of preventing and treating alcoholic fatty liver ( Valansa et al, 2020 ) and various acute liver injuries ( Mahmoud et al, 2014a ; Mahmoud et al, 2014b ; Yang et al, 2021 ). But the therapeutic potential of limonin in NAFLD is still unknown.…”

Section: Discussionmentioning

confidence: 99%

Limonin Alleviates Non-alcoholic Fatty Liver Disease by Reducing Lipid Accumulation, Suppressing Inflammation and Oxidative Stress

Yang

Lin

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease and continues to rise in the worldwide. Limonin is a triterpenoid compound widely found in the fruits of citrus plants with a wide range of pharmacological effects, including anti-cancer, anti-inflammation, anti-viral, anti-oxidation and liver protection properties. However, the potential molecular mechanism of limonin on NAFLD in zebrafish remains unknown. In this study, zebrafish larvae were exposed to thioacetamide to establish an NAFLD model and the larvae were treated with limonin for 72 h simultaneously. The human liver cell line was stimulated with lipid mixture and meanwhile incubated with limonin for 24 h. The results showed that Limonin significantly reduced the accumulation of lipid droplets in the liver and down-regulated the levels of lipogenic transcription factors FASN and SREBP1 in NAFLD. Limonin suppressed macrophages infiltration and the down-regulated the relative expression levels of the pro-inflammatory factors IL-6, IL-1β and TNF-α secreted by macrophages. Besides, limonin could reversed the reduction of glutathione and the accumulation of reactive oxygen species through up-regulating NRF2/HO-1 signaling pathway in the liver. In conclusion, this study revealed that limonin has a protective effect on NAFLD due to its resistance to lipid deposition as well as antioxidant and anti-inflammatory actions.

show abstract

Section: Discussionmentioning

confidence: 99%

Limonin Alleviates Non-alcoholic Fatty Liver Disease by Reducing Lipid Accumulation, Suppressing Inflammation and Oxidative Stress

Yang

Lin

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…Furthermore, the literature indicated that limonin characterised by ZJP exhibited protective activities against long-term alcohol injury due to antioxidant and anti-inflammatory mechanisms (Valansa et al. 2020 ). Based on these results in this study and the literature on various compounds in ZJP, it can be concluded that the gastroprotective activities of ZJP were attributed to the cooperation of its multiple compounds identified by UPLC-Q-Orbitrap HRMS in this study.…”

Section: Discussionmentioning

confidence: 99%

Integrated serum pharmacochemistry and investigation of the anti-gastric ulcer effect of Zuojin pill in rats induced by ethanol

Zhang

Yin

et al. 2022

Pharmaceutical Biology

View full text Add to dashboard Cite

Context Zuojin Pill (ZJP) has been used to treat gastrointestinal problems in China for hundreds of years. Objective To discover more potential active ingredients and evaluate the gastroprotective mechanisms of ZJP. Materials and methods An approach involving UPLC-Q-Orbitrap HRMS and serum pharmacochemistry was established to screen the multiple chemical constituents of ZJP. Male Sprague-Dawley (SD) rats were divided into six groups: normal control, ulcer control, omeprazole (30 mg/kg), and three ZJP groups (1.0, 2.0 and 4.0 g/kg). After oral treatment with ZJP or omeprazole for 7 days, all groups except the normal control group were orally administered 5 mL/kg ethanol to induce gastric ulceration. Histopathological assessment of gastric tissue was performed by haematoxylin and eosin staining. Antioxidant parameters and inflammatory mediators were determined using ELISA Kit and immunohistochemical analysis. Results Ninety components were identified in ZJP. Among them, 23 prototypes were found in rat serum after oral administration of ZJP. The ulcer inhibition was over 90.0% for all the ZJP groups. Compared with the ulcer control rats, ZJP (4.0 g/kg) enhanced the antioxidant capacity of gastric tissue: superoxide dismutase (1.33-fold), catalase (2.61-fold), glutathione (2.14-fold), and reduced the malondialdehyde level (0.48-fold). Simultaneously, the ZJP meaningfully lowered the content of tumour necrosis factor-α (0.76-fold), interleukin-6 (0.66-fold), myeloperoxidase (0.21-fold), and nuclear factor kappa B (p65) (0.62-fold). Discussion and conclusions This study showed ZJP could mitigate ethanol-induced rat gastric ulcers, which might benefit from the synergistic actions of multiple ingredients. The findings could support the quality control and clinical trials of ZJP.

show abstract

“…These ndings indicate that limonoids have a protective effect on liver injury in mice. 408 In the past decade, the skin protective effect of Meliaceae plant extracts have also been further discovered. Studies have shown that nimbolide can improve the bleomycin-induced scleroderma in mice by reducing the level of collagen crosslink LOXL2, and exerting antibrosis and antiinammatory activities.…”

Section: Reviewmentioning

confidence: 99%