Natural Leukocyte Membrane-Masked Microelectrodes with an Enhanced Antifouling Ability and Biocompatibility for <i>In Vivo</i> Electrochemical Sensing

Wei, Huan; Wu, Fei; Li, Lijuan; Yang, Xiaoti; Xu, Cong; Yu, Ping; Ma, Furong; Mao, Lanqun

doi:10.1021/acs.analchem.0c02240

Cited by 51 publications

(33 citation statements)

References 47 publications

(63 reference statements)

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“…Chemical approaches use highly hydrophilic systems to create a hydration layer that is resistant to nonspecific protein adsorption and reduces inflammatory responses. For example, hydrophilic leukocyte membranes and conducting polymers tailored with zwitterionic phosphorylcholine have been demonstrated to prevent biofouling in CFM in vivo [160,161]. Physical approaches rely on the engineering of filtration membranes or porous electrodes that exhibit size-related diffusion restrictions.…”

Section: In Vivo Challenges Of Electrochemical Sensorsmentioning

confidence: 99%

Recent Advances in In Vivo Neurochemical Monitoring

Tan

Robbins

et al. 2021

Micromachines

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The brain is a complex network that accounts for only 5% of human mass but consumes 20% of our energy. Uncovering the mysteries of the brain’s functions in motion, memory, learning, behavior, and mental health remains a hot but challenging topic. Neurochemicals in the brain, such as neurotransmitters, neuromodulators, gliotransmitters, hormones, and metabolism substrates and products, play vital roles in mediating and modulating normal brain function, and their abnormal release or imbalanced concentrations can cause various diseases, such as epilepsy, Alzheimer’s disease, and Parkinson’s disease. A wide range of techniques have been used to probe the concentrations of neurochemicals under normal, stimulated, diseased, and drug-induced conditions in order to understand the neurochemistry of drug mechanisms and develop diagnostic tools or therapies. Recent advancements in detection methods, device fabrication, and new materials have resulted in the development of neurochemical sensors with improved performance. However, direct in vivo measurements require a robust sensor that is highly sensitive and selective with minimal fouling and reduced inflammatory foreign body responses. Here, we review recent advances in neurochemical sensor development for in vivo studies, with a focus on electrochemical and optical probes. Other alternative methods are also compared. We discuss in detail the in vivo challenges for these methods and provide an outlook for future directions.

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Section: In Vivo Challenges Of Electrochemical Sensorsmentioning

confidence: 99%

Recent Advances in In Vivo Neurochemical Monitoring

Tan

Robbins

et al. 2021

Micromachines

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“…Although it is widely applicable, the nonspecific absorption of aptamers on gold surfaces usually leads to poor control over the orientation and density [14] . Moreover, CFEs featuring excellent electrochemical activities and high biocompatibility have been mostly employed as implanted sensing devices for probing neurochemicals in the CNS with excellent spatiotemporal resolution [15] . Although the pretreatment of carbon surfaces to produce functional groups, such as azide, [16] carboxylate groups, or highly cationic groups, enables the covalent coupling of aptamers through “click chemistry”, [17] 1‐ethyl‐3‐(3‐dimethylaminopropyl)carbodiimide/ N ‐hydroxysuccinimide (EDC/NHS) coupling, [18] or noncovalent assembling, [19] these methods usually require redox‐active catalysts, a long time, and often show low coupling efficiencies.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Conjugation of Aptamers on a Carbon Fiber Microelectrode Enables Highly Stable and Selective In Vivo Neurosensing

Jia

Zhu

et al. 2022

Angewandte Chemie

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Interfacing aptamers with carbon fiber microelectrodes (CFEs) provides a versatile platform to probe the chemical activity in a living brain at the molecular level. However, new approaches are needed for the efficient and stable modification of electrode surfaces with aptamers. Here, we present an electrochemical conjugation strategy to covalently couple aptamers onto CFEs with high chemoselectivity, efficiency, and stability for sensing in the brain. The strategy employs an initial electrochemical coupling of catechol on an CFE, thereby generating a thin layer of quinone intermediates that couple rapidly with thiol-containing oligonucleotides under a controlled potential. This approach dramatically simplifies and improves the efficiency for modifying carbon surfaces, thereby allowing direct conjugation of high levels of aptamers on CFEs within 5 minutes. Importantly, the covalent linkage between the aptamers and carbon surfaces enables a greatly improved sensitivity and stability for the sensing of dopamine, offering a robust system for continuously probing dopamine dynamics in the living brains of animals.

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“…By contrast, the electrochemical immunosensor has great advantages. It makes full use of the advantages of specific reaction of antigen and antibody and owns characteristics of rapid response and simple operation as well as low cost. − However, electrochemical immunosensor cannot detect CEA in the early stage of cancer due to low sensitivity. Thus, it is urgent to improve the sensitivity of electrochemical immunosensor.…”

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confidence: 99%

An Immunosensor Using Electroactive COF as Signal Probe for Electrochemical Detection of Carcinoembryonic Antigen

Liang

Luo

et al. 2022

Anal. Chem.

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Two kinds of two-dimensional (2D) covalentorganic frameworks (COF) were used to construct a sandwichtype electrochemical immunosensor for a proof-of-concept study. Vinyl-functionalized COFTab-Dva could be linked with Ab1 by the thiol−ene "click" reaction. Electroactive COFTFPB-Thi was modified with gold nanoparticles (AuNPs) to ensure the successful connection with Ab2 through Au−S bond. Meanwhile, electroactive COFTFPB-Thi was used to as signal probe to realize both the detection of carcinoembryonic antigen (CEA) and the amplification of detection signal. In detection process of the sandwich-type electrochemical immunosensor, glassy carbon electrode (GCE) was modified with 2D COFTab-Dva first then connected with Ab1 by the thiol−ene "click" reaction, next quantitative CEA was captured, followed by specificially capturing signal probe of Ab2/AuNPs/COFTFPB-Thi where AuNPs acted as nanocarriers of Ab2 and COFTFPB-Thi served as the signal producers. As the amount of CEA was increased, the amount of signal probe captured to the electrode was also increased, and the peak signal intensity of the redox reaction of COFTFPB-Thi was enhanced accordingly. Thus, the quantitative detection of CEA could be realized according to the peak signal intensity of electroactive COFTFPB-Thi. The electrochemical immunosensor owned wide detection range of 0.11 ng/mL-80 ng/mL, low detection limit of 0.034 ng/mL and good practicability. This study opens up a new revelation for quantitative detection of CEA using electroactive COF as enhanced signal probe.

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Natural Leukocyte Membrane-Masked Microelectrodes with an Enhanced Antifouling Ability and Biocompatibility for In Vivo Electrochemical Sensing

Cited by 51 publications

References 47 publications

Recent Advances in In Vivo Neurochemical Monitoring

Recent Advances in In Vivo Neurochemical Monitoring

Electrochemical Conjugation of Aptamers on a Carbon Fiber Microelectrode Enables Highly Stable and Selective In Vivo Neurosensing

An Immunosensor Using Electroactive COF as Signal Probe for Electrochemical Detection of Carcinoembryonic Antigen

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