Natural Killer Susceptibility of Brain Tumor Cell Lines Inversely Correlates with the Degree of Cell Differentiation and Not with the Level of Human Histocompatibility Antigen Expression

Alonso, Corona; Serrano, Rafael; Solana, Rafael; Peña, José

doi:10.1159/000234941

Cited by 6 publications

(2 citation statements)

References 14 publications

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“…Twenty cell lines derived from human tumors, obtained and characterized in our laboratory [23,24], were used.Ten cell lines were derived from primitive brain tumors (seven gliomas and three meningosarcomas); five were brain metastases of different origins (one uterus fibrosarcoma, one melanoma, one colonic adenocarcinoma and two lung adenocarcinomas). Another five cell lines were obtained from pleural metastasis of four lung adenocarcinomas and one breast adenocarcinoma.…”

Section: Cell L I E Smentioning

confidence: 99%

Natural killer susceptibility is independent of HLA class I antigen expression on cell lines obtained from human solid tumors

et al. 1990

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The susceptibility to natural killer (NK) cell-mediated cytotoxicity of 20 cell lines obtained from human solid tumors and their class I histocompatibility antigen (HLA) levels were studied in an attempt to determine whether major histocompatibility complex (MHC) products expressed on cells derived from human solid tumors influence NK susceptibility. The effect of interferon-gamma (IFN-gamma) treatment on these elements was also analyzed. The MHC class I (HLA-ABC, HLA-A and HLA-B) antigen levels and degree of NK lysis were very heterogeneous and no correlation was found on comparison. After treatment with IFN-gamma a marked decrease in NK susceptibility was observed in all the cell lines, including the control line K-562. However, the level of HLA class I expression was not modified in any of the lines with the exception of the K-562, which increased. In some cell lines the expression of HLA class I-like antigens. CDla, b and c, was also measured before and after IFN treatment; however, no correlation was found between CD1 levels and NK susceptibility. Consequently, from our results it is possible to conclude that HLA class I antigens do not play a decisive role in NK susceptibility of cell lines derived from human solid tumors and to suggest that molecules which are not HLA class I antigens but IFN-gamma inducible may confer NK resistance to these lines.

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Section: Cell L I E Smentioning

confidence: 99%

Natural killer susceptibility is independent of HLA class I antigen expression on cell lines obtained from human solid tumors

et al. 1990

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“…These include, for example, a defect in the recognition of target cells with a relationship between HLA antigen expression and tumour cell performance (Stern et al, 1980;Karre et al, 1986;Powel et al, 1987;Rodger et al, 1988;Alonso et al, 1989;Ljnggren & Karre, 1990;Solana et al, 1990b;Peiia et al, 1990a,b); a resistance of target cells to lytic factors such as TNF or NKCF produced by immunocompetent cells (Roozemond et al, 1987); or an inhibited NK activity owing to tumour suppressor factors or peptides such as prostaglandin E2 and NK-RIF (Harris et al, 1987;Serrano et al, 1989Serrano et al, , 1990Solana et al, 1990a). However, the exact mechanisms by which NK cells interact with target cells remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Sera from patients with colon, breast and lung cancer induce resistance to lysis mediated by NK cytotoxic factors (NKCF)

Marubayashi

Solana²,

Ramı́rez³

et al. 1991

Br J Cancer

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Summary Natural killer (NK) cells are involved in the antitumoral immunologic mechanism. These cells act through the release of cytotoxic molecules defined as NK cytotoxic factors (NKCF). Inhibitory factors of NK and NKCF mediated lysis have been described in in vitro assays. This study evaluates the induction of resistance to NKCF cytotoxicity by sera from 27 patients with colon, breast and lung cancer. Addition of these sera to the cytolytic assay where K562 cells and concentrated NKCF were used, induced resistance to NKCF mediated cytotoxicity in 21 cases (77%). The sera from the group with metastasis blocked NKCF lysis more markedly than the group with local tumours. However, no differences were observed when the groups with colon, breast and lung cancers were compared. This blocking effect was not found to be related to gamma interferon (IFN) levels. In a previous study, we described a tumour factor (NK-RIF) produced by human cell lines derived from metastatic adenocarcinomas. This factor blocked lysis of tumour target cells by NK cells. Consequently, it is proposed that the release of similar tumour factors with a capacity to induce resistance to NKCF may be involved in tumour growth and metastatic spreading in in vivo.

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Histocompatibility antigens and natural killer susceptibility

Peña

Solana

1992

Immunol Res

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The central question of the nature of the structure(s) involved in the recognition of targets by natural killer (NK) cells remains unresolved. Although NK-mediated cytotoxicity is not MHC-restricted, it has been suggested that these cells could recognize the targets more effectively in the absence of MHC class I antigens. In this paper we review the contradictory results obtained when studying the NK susceptibility of cell lines which constitutively express different levels of MHC antigens, or which have been induced to express MHC antigens by gene transfection or gamma-interferon treatment. Taken together, the results indicate that MHC antigens play a differential role in NK lysis depending on the nature of the target cells used; MHC class I antigens play a role in the NK resistance of cells from a hematopoietic lineage, but this does not extend to cells from other origins. The data reviewed also support the hypothesis that MHC class I antigens induced NK resistance by interfering with target structures, and that multiple NK molecules are involved in NK-mediated lysis as part of a possible advanced recognition system.

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Natural Killer Susceptibility of Brain Tumor Cell Lines Inversely Correlates with the Degree of Cell Differentiation and Not with the Level of Human Histocompatibility Antigen Expression

Cited by 6 publications

References 14 publications

Natural killer susceptibility is independent of HLA class I antigen expression on cell lines obtained from human solid tumors

Natural killer susceptibility is independent of HLA class I antigen expression on cell lines obtained from human solid tumors

Sera from patients with colon, breast and lung cancer induce resistance to lysis mediated by NK cytotoxic factors (NKCF)

Histocompatibility antigens and natural killer susceptibility

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