Natural-Killer-like B Cells Display the Phenotypic and Functional Characteristics of Conventional B Cells

Kerdiles, Yann M.; Almeida, Francisca F.; Thompson, Thornton W.; Chopin, Michaël; Vienne, Margaux; Bruhns, Pierre; Huntington, Nicholas D.; Raulet, David H.; Nutt, Stephen L.; Belz, Gabrielle T.; Vivier, Éric

doi:10.1016/j.immuni.2017.07.026

Cited by 17 publications

(16 citation statements)

References 10 publications

(16 reference statements)

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“…Our data suggest that the NKB cell phenotype, regardless of unknown ontogeny is likely a real one. However, much as was previously suggested [17] , we speculate NKB cells are a subpopulation of B cells that unexpectedly shares some properties overlapping with NK cells, the functional reasons for which remain unclear. Strikingly these cells are also significantly perturbed during lentivirus infection.…”

Section: Discussionsupporting

confidence: 57%

“…Natural killer-like B cells (NKB), like other ILB, also have semi-permanent expression of natural IgM, can activate NK and innate lymphoid cells following stimulation, and thus modulate a critical cascade of innate and adaptive immune responses eventually necessary to contain viral infections. However, Kerdiles et al [17] questioned these findings and suggested that NKB in mice may not actually be a unique subset of B cell, but instead are just a subpopulation of conventional B cells. In rebuttal, Wang et al [18] reported mRNA expression of genes encoding NK1.1 (klrb1c) and NKp46 (Ncr1) in murine NKB cells.…”

Section: Introductionmentioning

confidence: 99%

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Progressive lentivirus infection induces natural killer cell receptor-expressing B cells in the gastrointestinal tract

Manickam

Nwanze

Ram

et al. 2018

Aids

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Objective Recently, a seemingly novel innate immune cell subset bearing features of natural killer and B cells was identified in mice. So-called NKB cells appear as first responders to infections, but whether this cell population is truly novel or is in fact a subpopulation of B cells and exists in higher primates remains unclear. The objective of this study was to identify NKB cells in primates and study the impact of HIV/SIV infections. Design and Methods NKB cells were quantified in both naïve and lentivirus infected rhesus macaques and humans by excluding lineage markers (CD3, CD127), and positive Boolean gating for CD20, NKG2A/C and/or NKp46. Additional phenotypic measures were conducted by RNA-probe and traditional flow cytometry. Results Circulating cytotoxic NKB cells were found at similar frequencies in humans and rhesus macaques (range, 0.01 to 0.2% of total lymphocytes). NKB cells were notably enriched in spleen (median, 0.4% of lymphocytes), but were otherwise systemically distributed in tonsil, lymph nodes, colon, and jejunum. Expression of immunoglobulins was highly variable, but heavily favoured IgM and IgA rather than IgG. Interestingly, NKB cell frequencies expanded in PBMC and colon during SIV infection, as did IgG expression, but were generally unaltered in HIV-infected human subjects. Conclusion These results suggest a cell type expressing both NK and B cell features exists in rhesus macaques and humans and are perturbed by HIV/SIV infection. The full functional niche remains unknown, but the unique phenotype and systemic distribution could make NKB cells unique targets for immunotherapeutics or vaccine strategies.

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Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Progressive lentivirus infection induces natural killer cell receptor-expressing B cells in the gastrointestinal tract

Manickam

Nwanze

Ram

et al. 2018

Aids

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“…Fascinatingly, unexpected and very recent data identify a previously unknown B lymphocyte subpopulation that shares some NK cells features. This subset now called NK‐like B (NKB) cells is characterized by the expression of B cell‐associated molecules such as a fully rearranged BCR, the B cell lineage marker CD19, and MHC‐II molecules along with the classic NK cell markers CD161 (NK1.1) and CD335 (NKp46) . Interestingly, they are also characterized by a high expression of molecules neither related with B or NK lineages, including the tetraspanin CD63 (TSPAN30) or the endothelial adhesion molecule CD106 (VCAM‐1) .…”

Section: Nk‐like B Cellsmentioning

confidence: 99%

“…Interestingly, they are also characterized by a high expression of molecules neither related with B or NK lineages, including the tetraspanin CD63 (TSPAN30) or the endothelial adhesion molecule CD106 (VCAM‐1) . However, NKBs lack of the expression of effector molecule perforin and their cytoplasm do not contain cytotoxic granules, both characteristics implicating that they do not possess inherent cytotoxic activity …”

Section: Nk‐like B Cellsmentioning

confidence: 99%

“…This subset now called NK-like B (NKB) cells is characterized by the expression of B cell-associated molecules such as a fully rearranged BCR, the B cell lineage marker CD19, and MHC-II molecules along with the classic NK cell markers CD161 (NK1.1) and CD335 (NKp46). 128 Interestingly, they are also characterized by a high expression of molecules neither related with B or NK lineages, including the tetraspanin CD63 (TSPAN30) or the endothelial adhesion molecule CD106 (VCAM-1). 129 However, NKBs lack of the expression of effector molecule perforin and their cytoplasm do not contain cytotoxic granules, both characteristics implicating that they do not possess inherent cytotoxic activity.…”

Section: Nk-like B Cellsmentioning

confidence: 99%

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Innate-like B cell subsets during immune responses: Beyond antibody production

Romero‐Ramírez

Navarro-Hernandez

Cervantes‐Díaz

et al. 2018

Journal of Leukocyte Biology

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B lymphocytes are recognized for their crucial role in the adaptive immunity since they represent the only leukocyte lineage capable of differentiating into Ab‐secreting cells. However, it has been demonstrated that these lymphocytes can exert several Ab‐independent functions, including engulfing and processing Ags for presentation to T cells, secreting soluble mediators, providing co‐stimulatory signals, and even participating in lymphoid tissues development. Beyond that, several reports claiming the existence of multiple B cell subsets contributing directly to innate immune responses have appeared. These “innate‐like” B lymphocytes, whose phenotype, development pathways, tissue distribution, and functions are in most cases notoriously different from those of conventional B cells, are crucial to early protective responses against pathogens by exerting “crossover” defensive strategies that blur the established boundaries of innate and adaptive branches of immunity. Examples of these mechanisms include the rapid secretion of the polyspecific natural Abs, increased susceptibility to innate receptors‐mediated activation, cytokine secretion, downstream priming of other innate cells, usage of specific variable immunoglobulin gene‐segments, and other features. As these new insights emerge, it is becoming preponderant to redefine the functionality of B cells beyond their classical adaptive‐immune tasks.

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Application of Independent Component Analysis to Tumor Transcriptomes Reveals Specific and Reproducible Immune-Related Signals

Czerwińska

Cantini

Kairov

et al. 2018

Latent Variable Analysis and Signal Separation

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Natural-Killer-like B Cells Display the Phenotypic and Functional Characteristics of Conventional B Cells

Cited by 17 publications

References 10 publications

Progressive lentivirus infection induces natural killer cell receptor-expressing B cells in the gastrointestinal tract

Progressive lentivirus infection induces natural killer cell receptor-expressing B cells in the gastrointestinal tract

Innate-like B cell subsets during immune responses: Beyond antibody production

Application of Independent Component Analysis to Tumor Transcriptomes Reveals Specific and Reproducible Immune-Related Signals

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