Natural Killer Cell Receptor Expression by Human First Trimester Decidual Granular Leukocytes and T‐Lymphocytes

Lewis, B. J.; Croker, Simon; Newton, Darren J.; Lennon, Greig P.; Johnson, Peter; Christmas, Stephen E.

doi:10.1034/j.1600-0897.2002.01115.x

Cited by 11 publications

(10 citation statements)

References 30 publications

(43 reference statements)

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“…NKp30 mRNA and protein are known to be expressed by first trimester decidual uterine NK cells [11,12]. Data from our transcriptional profiling of human endometrium showed that NKp30 mRNA was significantly upregulated during the late-secretory stage of the menstrual cycle [1].…”

Section: Introductionmentioning

confidence: 84%

“…Given that NKp30 is expressed by both resting and NK cells and it has been shown to be expressed by decidual uNK cells [11,12], it was surprising to find no uNK immune reactivity in the stroma of late-secretory phase endometrium by IHC (even though there were many uNK cells present, Fig. 2N).…”

Section: Discussionmentioning

confidence: 99%

“…The mRNA was significantly up-regulated during the late-secretory phase of the menstrual cycle, when there is a significant infiltration of NK cells into the endometrium. Since NKp30 is expressed by both resting and activated NK cells, and studies have shown that decidual uterine NK (uNK) cells also express NKp30 [11,12], our initial thoughts were that the significant increase of NKp30 mRNA during the late-secretory stage was due to the increase in uNK cell numbers at this time. It was thus surprising to find NKp30 mRNA in CEE at the same magnitude as freshly frozen endometrial tissue samples.…”

Section: Discussionmentioning

confidence: 99%

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Identification and hormonal regulation of a novel form of NKp30 in human endometrial epithelium

Ponnampalam¹,

Gargett²,

Rogers³

2007

Eur J Immunol

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This study reports the discovery and steroid hormonal regulation of a novel glycosylated form of NKp30 in human endometrial epithelium. NKp30 is a member of the immunoglobulin superfamily and one of three existing natural cytotoxicity-triggering receptors. NKp30 is a glycosylated protein and is thought to be selectively expressed in resting and activated natural killer cells. The aims of the present study were to fully characterize NKp30 mRNA and protein in human endometrium during the menstrual cycle, and to investigate the hormonal regulation of NKp30. NKp30 mRNA was significantly up-regulated in fresh tissues during late secretory phase of the menstrual cycle. Interestingly, NKp30 mRNA was also present in clonally derived endometrial epithelial cells (CEE) in comparable amounts to fresh tissue. NKp30 protein was predominantly found in the endometrial glands and luminal epithelia of the secretory phase endometrium. Western blotting and de-glycosylation studies show that a novel glycosylated form of NKp30 is present in endometrial epithelium and that it can dimerize. Further phenotyping of CEE by flow cytometry revealed that they are CK8 + CD49f + NKp30 + CD45 -CD56 -. The data also show that transcription and translation of the novel form of NKp30 can be induced by progesterone treatment after 48 h in endometrial explants in vitro. This is the first report to show the presence of both NKp30 mRNA and a novel glycosylated form of NKp30 protein in endometrial epithelial cells.

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Section: Introductionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and hormonal regulation of a novel form of NKp30 in human endometrial epithelium

Ponnampalam¹,

Gargett²,

Rogers³

2007

Eur J Immunol

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“…Previous studies of KIR expression in uNK cells used patients whose genotype was not known (5,6,26). In addition the Abs used were not able to discriminate between activating and inhibitory KIR.…”

Section: Discussionmentioning

confidence: 99%

“…In an immunogenetic study, we showed that specific combinations of two polymorphic gene systems, killer Ig-like receptors (KIR) in the mother and HLA-C in the fetus were associated with pre-eclampsia (4). In the decidua, KIR are expressed only by uNK cells and their cognate ligands include HLA-C molecules that are expressed by trophoblast (5)(6)(7)(8).…”

mentioning

confidence: 99%

Killer Ig-Like Receptor Expression in Uterine NK Cells Is Biased toward Recognition of HLA-C and Alters with Gestational Age

Sharkey

Gardner

Hiby

et al. 2008

The Journal of Immunology

135

119

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Immunogenetic studies suggest that interactions between maternal killer Ig-like receptor (KIR) expressed by uterine NK (uNK) cells, and fetal HLA-C molecules on trophoblast, influence the success of human placentation. However, the exact functional response of fresh uNK cells to trophoblast HLA-C molecules is unknown. In this study, we show by quantitative RT-PCR and FACS that both activating and inhibitory KIR specific for HLA-C are expressed at higher levels and on an increased proportion of NK cells in the human decidua compared with blood. In contrast, expression of KIR3DL1/S1, which is specific for HLA-B, is similar in both NK cell populations. Remarkably, there is also a temporal change in the expression pattern of HLA-C-specific KIR, with a decline in both intensity of expression and frequency on uNK cells throughout the first trimester of pregnancy. This selective up-regulation of KIR has functional consequences because uNK cells show increased binding of HLA-C tetramers compared with blood NK cells. Ab cross-linking shows that these KIR are functional and results in increased cytokine secretion. uNK cells, therefore, exhibit a unique KIR profile that enhances their ability to recognize trophoblast cells expressing HLA-C at the materno-fetal interface. This is the first report to demonstrate selective regulation of KIR expression over time in vivo in a normal physiological situation and suggests that KIR expression by uNK cells is regulated by the tissue microenvironment in the decidua.

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Role of a KIR/HLA-C allorecognition system in pregnancy

Varla-Leftherioti¹

2004

Journal of Reproductive Immunology

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Natural Killer Cell Receptor Expression by Human First Trimester Decidual Granular Leukocytes and T‐Lymphocytes

Cited by 11 publications

References 30 publications

Identification and hormonal regulation of a novel form of NKp30 in human endometrial epithelium

Identification and hormonal regulation of a novel form of NKp30 in human endometrial epithelium

Killer Ig-Like Receptor Expression in Uterine NK Cells Is Biased toward Recognition of HLA-C and Alters with Gestational Age

Role of a KIR/HLA-C allorecognition system in pregnancy

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