Natural Interactions between<i>S. haematobium</i>and<i>S. guineensis</i>in the Republic of Benin

Moné, Hélène; Minguez, Stéphanie; Ibikounlé, Moudachirou; Allienne, Jean‐François; Massougbodji, Achille; Mouahid, Gabriel

doi:10.1100/2012/793420

Cited by 40 publications

(38 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To our knowledge, S. intercalatum has never been reported in Benin, whereas S. guineensis was mentioned in only one publication. 26 A similar case-of uncertain diagnosis-was described in Gabon in 2005 by N'Dong and others, but the authors concluded to a S. haematobium lung disease considering epidemiological, pathophysiological, and morphological criteria, in the absence of molecular study and despite the positive ZiehlNeelsen stain. 27 Ultimately, the possibility of a hybrid species involvement such as S. haematobium/S.…”

Section: Discussionmentioning

confidence: 82%

“…30 Furthermore, interestingly, the molecular evidence of hybridization between S. haematobium and S. guineensis was described for the first time in Benin in 2012. 26 In conclusion, this case is remarkable for the rarity of pulmonary schistosomiasis, its peculiar clinical presentation, and the difficulty to identify the species. Because the exact genetic nature of the eggs was not determined, we cannot exclude the possibility of an infection with S. intercalatum (never reported in Benin) or with a hybrid species such as S. haematobium/S.…”

Section: Discussionmentioning

confidence: 85%

“…22 Transmission of these two anthropophilic species occurs almost exclusively in restricted areas of central Africa. [23][24][25][26] Sporadic cases among travelers have also been reported across west Africa (Burkina Faso, Tchad, Mali, and Nigeria), but no transmission focus was precisely identified. To our knowledge, S. intercalatum has never been reported in Benin, whereas S. guineensis was mentioned in only one publication.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Early Detection of Schistosoma Egg–Induced Pulmonary Granulomas in a Returning Traveler

Coron¹,

Govic²,

Kettani³

et al. 2016

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. We report the case of a French traveler who developed acute pulmonary schistosomiasis 2 months after visiting Benin. He presented with a 1-month history of fever, cough, and thoracic pain. Initial investigations revealed hypereosinophilia and multiple nodular lesions on chest computed tomography scan. Lung biopsies were performed 2 months later because of migrating chest infiltrates and increasing eosinophilia. Histological examination showed schistosomal egg-induced pulmonary granulomas with ova exhibiting a prominent terminal spine, resembling Schistosoma haematobium. However, egg shells were Ziehl-Neelsen positive, raising the possibility of a Schistosoma intercalatum or a Schistosoma guineensis infection. Moreover, involvement of highly infectious hybrid species cannot be excluded considering the atypical early pulmonary oviposition. This case is remarkable because of the rarity of pulmonary schistosomiasis, its peculiar clinical presentation and difficulties in making species identification. It also emphasizes the need to consider schistosomiasis diagnosis in all potentially exposed travelers with compatible symptoms.

show abstract

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Early Detection of Schistosoma Egg–Induced Pulmonary Granulomas in a Returning Traveler

Coron¹,

Govic²,

Kettani³

et al. 2016

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…We suspect this phenomenon is more common than generally reported but evidence of this exists for only a few trematode taxa (e.g. Mone et al, 2012;Diaz et al, 2015).…”

Section: Sequencingmentioning

confidence: 79%

Molecular approaches to trematode systematics: ‘best practice’ and implications for future study

et al. 2016

View full text Add to dashboard Cite

'Molecular approaches to trematode systematics: 'best practice' and implications for future study ', Systematic Parasitology, 93(3), 295-306. The final publication is available at Springer via http://dx.doi.org/10.1007/s11230-016-9631-2.The full details of the published version of the article are as follows: However, since the late-1980s we have seen an increased integration of genetic data to overcome problems encountered when morphological data are considered in isolation. Here, we provide advice regarding the 'best molecular practice' for trematode taxonomy and systematic studies, in an attempt to help unify the field and provide a solid foundation to underpin future work.Emphasis is placed on defining the study goals and recommendations are made regarding sample preservation, extraction methods, and the submission of molecular vouchers. We advocate generating sequence data from all parasite species/host species/geographic location combinations and stress the importance of selecting two independently evolving loci (one ribosomal and one mitochondrial marker). We recommend that loci should be chosen to provide genetic variation suitable to address the question at hand and for which sufficient 'useful' comparative sequence data already exist. Quality control of the molecular data via using proof-reading Taq polymerase, sequencing PCR amplicons using both forward and reverse primers, ensuring that a minimum of 85% overlap exists when constructing consensus sequences, and checking electropherograms by eye is stressed. We advise that all genetic results are best interpreted using a holistic biological approach, which considers morphology, host identity, collection locality, and ecology. Finally, we consider what advances next-generation sequencing holds for trematode taxonomy and systematics.

show abstract

“…haematobium x S . guineensis [33,34], between schistosome species infecting animals ( S . bovis x S .…”

Section: Discussionmentioning

confidence: 99%

A Genome Wide Comparison to Identify Markers to Differentiate the Sex of Larval Stages of Schistosoma haematobium, Schistosoma bovis and their Respective Hybrids

Kincaid-Smith¹,

Boissier²,

Allienne³

et al. 2016

PLoS Negl Trop Dis

Self Cite

View full text Add to dashboard Cite

For scientists working on gonochoric organisms, determining sex can be crucial for many biological questions and experimental studies, such as crossbreeding, but it can also be a challenging task, particularly when no sexual dimorphism is visible or cannot be directly observed. In metazoan parasites of the genus Schistosoma responsible for schistosomiasis, sex is genetically determined in the zygote with a female heterogametic ZW/ZZ system. Adult flukes have a pronounced sexual dimorphism, whereas the sexes of the larval stages are morphologically indistinguishable but can be distinguished uniquely by using molecular methods. Therefore, reliable methods are needed to identify the sex of larvae individuals. Here, we present an endpoint PCR-based assay using female-specific sequences identified using a genome-wide comparative analysis between males and females. This work allowed us to identify sex-markers for Schistosoma haematobium and Schistosoma bovis but also the hybrid between both species that has recently emerged in Corsica (France). Five molecular sex-markers were identified and are female-specific in S. haematobium and the hybrid parasite, whereas three of them are also female-specific in S. bovis. These molecular markers will be useful to conduct studies, such as experimental crosses on these disease-causing blood flukes, which are still largely neglected but no longer restricted to tropical areas.

show abstract

Natural Interactions betweenS. haematobiumandS. guineensisin the Republic of Benin

Cited by 40 publications

References 30 publications

Early Detection of Schistosoma Egg–Induced Pulmonary Granulomas in a Returning Traveler

Early Detection of Schistosoma Egg–Induced Pulmonary Granulomas in a Returning Traveler

Molecular approaches to trematode systematics: ‘best practice’ and implications for future study

A Genome Wide Comparison to Identify Markers to Differentiate the Sex of Larval Stages of Schistosoma haematobium, Schistosoma bovis and their Respective Hybrids

Contact Info

Product

Resources

About