Natural insertions within the N-terminal region of the coat protein of Maize dwarf mosaic potyvirus (MDMV) have an effect on the RNA stability

Petrik, Kathrin; Sebestyén, Endre; Gell, Gyöngyvér; Balázs, Ervin

doi:10.1007/s11262-009-0425-3

Cited by 13 publications

(7 citation statements)

References 18 publications

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“…Comparative computer-based analysis revealed that in some isolates the insertion exhibited a high degree of conservation both in terms of nucleic acid base sequence and localization. Further analysis suggested that the insertion plays a role in virus RNA stability [39].…”

Section: Recombination Analysismentioning

confidence: 99%

Recombination analysis of Maize dwarf mosaic virus (MDMV) in the Sugarcane mosaic virus (SCMV) subgroup of potyviruses

2014

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Recombination among RNA viruses is a natural phenomenon that appears to have played a significant role in the species development and the evolution of many strains. It also has particular significance for the risk assessment of plants which have been genetically modified for disease resistance by incorporating viral sequences into their genomes. However, the exact recombination events taking place in viral genomes are not investigated in detail for many virus groups. In this analysis, different single-stranded positive-sense RNA potyviruses were compared using various in silico recombination detection methods and new recombination events in the Sugarcane mosaic virus (SCMV) subgroup were detected. For an extended in silico recombination analysis, two of the analyzed Maize dwarf mosaic virus full-length genomes were sequenced additionally during this work. These results strengthen the evidence that recombination is a major driving force in virus evolution, and the emergence of new virus variants in the SCMV subgroup, paired with mutations, could generate viruses with altered biological properties. The intra- and interspecific homolog recombinations seem to be a general trait in this virus group, causing little or no changes to the amino acid of the progenies. However, we found a few breakpoints between the members of SCMV subgroup and the weed-infecting distant relatives, but only a few methods of the RDP3 package predicted these events with low significance level.

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Section: Recombination Analysismentioning

confidence: 99%

Recombination analysis of Maize dwarf mosaic virus (MDMV) in the Sugarcane mosaic virus (SCMV) subgroup of potyviruses

2014

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“…JQ280313.1), MDMV-Sz0605 (GenBank accession no. FM883211.2 [49], MDMV-Mv0801 (GenBank accession no. FM883164.2 [49] (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…FM883211.2 [49], MDMV-Mv0801 (GenBank accession no. FM883164.2 [49] (Table 1). RACE was also performed for MDMV-Italy (GenBank accession no.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Gene Expression and Multi-Gene Silencing in Zea Mays Using Maize Dwarf Mosaic Virus

Xie

Marty

et al. 2020

Preprint

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Background: Maize dwarf mosaic virus (MDMV), a member of the genus Potyvirus, infects maize and is non-persistently transmitted by aphids. Several plant viruses have been developed as tools for gene expression and gene silencing in plants. The capacity of MDMV for both gene expression and gene silencing were examined.Results: Infectious clones of an Ohio isolate of MDMV, MDMV-OH5, were obtained, and engineered for gene expression only, and for simultaneous marker gene expression and virus-induced gene silencing (VIGS) of three endogenous maize target genes. Single gene expression in single insertion constructs and simultaneous expression of green fluorescent protein (GFP) and silencing of three maize genes in a double insertion construct was demonstrated. Constructs with GFP inserted in the N-terminus of HCPro were more stable than those with insertion at the N-terminus of CP in our study. Unexpectedly, the construct with two insertion sites also retained insertions at a higher rate than single-insertion constructs. Engineered MDMV expression and VIGS constructs were transmissible by aphids (Rhopalosiphum padi). Conclusions: These results demonstrate that MDMV-based vector can be used as a tool for simultaneous gene expression and multi-gene silencing in maize.

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“…This protein subunit of MDMV has a molecular weight of 28.5 × 10 3 according to amino acid analyses [ 32 ]. The C-terminal regions of the coat protein primarily plays a role in the process of encapsidation and cell-to-cell transport and the flexible N-terminus is involved in long distance and systemic transport and contains the DAG motif essential for aphid transmission competence [ 33 ].…”

Section: Genomementioning

confidence: 99%

Maize Dwarf Mosaic Virus: From Genome to Disease Management

Kannan

Ismail

Bunawan

2018

Viruses

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Maize dwarf mosaic virus (MDMV) is a serious maize pathogen, epidemic worldwide, and one of the most common virus diseases for monocotyledonous plants, causing up to 70% loss in corn yield globally since 1960. MDMV belongs to the genus Potyvirus (Potyviridae) and was first identified in 1964 in Illinois in corn and Johnsongrass. MDMV is a single stranded positive sense RNA virus and is transmitted in a non-persistent manner by several aphid species. MDMV is amongst the most important virus diseases in maize worldwide. This review will discuss its genome, transmission, symptomatology, diagnosis and management. Particular emphasis will be given to the current state of knowledge on the diagnosis and control of MDMV, due to its importance in reducing the impact of maize dwarf mosaic disease, to produce an enhanced quality and quantity of maize.

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Natural insertions within the N-terminal region of the coat protein of Maize dwarf mosaic potyvirus (MDMV) have an effect on the RNA stability

Cited by 13 publications

References 18 publications

Recombination analysis of Maize dwarf mosaic virus (MDMV) in the Sugarcane mosaic virus (SCMV) subgroup of potyviruses

Recombination analysis of Maize dwarf mosaic virus (MDMV) in the Sugarcane mosaic virus (SCMV) subgroup of potyviruses

Simultaneous Gene Expression and Multi-Gene Silencing in Zea Mays Using Maize Dwarf Mosaic Virus

Maize Dwarf Mosaic Virus: From Genome to Disease Management

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