Natural infection of Lutzomyia trinidadensis (Diptera: Psychodidae) with Leishmania in Barquisimeto, Venezuela

Bonfante-Garrido, Rafael; Urdaneta, Rafael; Urdaneta, Iván; Alvarado, Jorge A.

doi:10.1590/s0074-02761990000400015

Cited by 11 publications

(4 citation statements)

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“…This may be explained by several factors and by taking into account the high genetic diversity observed in L. braziliensis and even the appearance of haplotypes as a result of their geographical distribution; it is possible that these genetic changes allowed these species to adapt to new hosts or vectors in domestic settings [9,12,56]. This concurs with existing reports from the San Cristobal municipality of the state of Táchira, Venezuela, which borders Norte de Santander and has the highest number of CL cases in Venezuela, in which L. braziliensis has been detected in patients and vectors [1,[35][36][37][57][58][59].…”

Section: Discussionsupporting

confidence: 82%

Complex ecological interactions across a focus of cutaneous leishmaniasis in Eastern Colombia: novel description of Leishmania species, hosts and phlebotomine fauna

et al. 2020

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This study aimed to analyse the patterns of diversity, blood sources and Leishmania species of phlebotomines in a focus of cutaneous leishmaniasis in Arboledas, Eastern Colombia. In total, 1729 phlebotomines were captured in two localities (62.3% Siravita and 37.7% Cinera) and five environments of Norte de Santander. We identified 18 species of phlebotomines: Pintomyia ovallesi (29.8%), Psychodopygus davisi (20.3%), Pi. spinicrassa (18.5%) and Lutzomyia gomezi (15.8%) showed the highest abundance. Species diversities were compared between Cinera (15.00) and Siravita (20.00) and among five microenvironments: forest remnants (19.49), coffee plantations (12.5), grassland (12.99), cane plantations (11.66) and citrus plantations (12.22). Leishmania DNA was detected in 5.8% (80/1380) of females, corresponding mainly to Pi . ovallesi (22/80; 27.2%), Lu . gomezi (17/80; 21.3%) and Pi . spinicrassa (11/80; 13.8%). Leishmania species were 63.1% L. braziliensis , 18.5% L . panamensis , 13.2% L . infantum and 6.1% L . amazonensis . The most frequent feeding sources were Homo sapiens (50%), Bos taurus (13.8%) and Canis lupus familiaris (10.3%). This focus of cutaneous leishmaniasis has a high diversity of Leishmania -carrying phlebotomines that feed on domestic animals. The transmission of leishmaniasis to human hosts was mainly associated with Lu . gomezi , Pi . ovallesi and L . braziliensis .

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Section: Discussionsupporting

confidence: 82%

Complex ecological interactions across a focus of cutaneous leishmaniasis in Eastern Colombia: novel description of Leishmania species, hosts and phlebotomine fauna

et al. 2020

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“…trinidadensis, the same sand fly species found to be Leishmania positive in our study but without an identifiable species. 33 However, it is unlikely that the Leishmania species found in Lu. auraensis could be a misclassification of other species.…”

Section: Discussionmentioning

confidence: 99%

Natural Leishmania Infection of Lutzomyia auraensis in Madre de Dios, Peru, Detected by a Fluorescence Resonance Energy Transfer–Based Real-Time Polymerase Chain Reaction

Valdivia

Santos²,

Fernández³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Leishmania species of the Viannia subgenus are responsible for most cases of New World tegumentary leishmaniasis. However, little is known about the vectors involved in disease transmission in the Amazon regions of Peru. We used a novel real-time polymerase chain reaction (PCR) to assess Leishmania infections in phlebotomines collected in rural areas of Madre de Dios, Peru. A total of 1,299 non-blood fed female sand flies from 33 species were captured by using miniature CDC light traps. Lutzomyia auraensis was the most abundant species (63%) in this area. Seven of 164 pools were positive by PCR for Leishmania by kinetoplast DNA. The real-time PCR identified four Lu. auraensis pools as positive for L. (Viannia) lainsoni and L. (V.) braziliensis. The minimum infection prevalence for Lu. auraensis was estimated to be 0.6% (95% confidence interval = 0.20-1.42%). Further studies are needed to assess the importance of Lu. auraensis in the transmission of New World tegumentary leishmaniasis in hyperendemic areas of Peru.

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“…In the evaluation of captured sand flies, the classical microscopic analysis and culture methods have been used to detect natural infection in sand flies, these techniques were performed in several studies [24, 25, 26, 27, 28]. However, the low sensitivity of these methods, the operational difficulty and delay to deal with a large number of samples and the low prevalence of L .…”

Section: Introductionmentioning

confidence: 99%

Natural infection by Leishmania infantum in the Lutzomyia longipalpis population of an endemic coastal area to visceral leishmaniasis in Brazil is not associated with bioclimatic factors

et al. 2019

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Visceral leishmaniasis (VL) is a zoonosis caused by the protozoan Leishmania infantum and in Brazil is transmitted mainly by the bite of Lutzomuyia longipalpis sand flies. Data about the presence, distribution, natural infection rate, seasonal and monthly dynamics of the vector population are important for optimizing the measures to control VL in endemic areas. This study aimed to identify sand fly fauna in an endemic area for VL to detect the prevalence of L . infantum infection in the Lu . longipalpis population and to elucidate the influence of bioclimatic factors on the monthly fluctuations of this vector. HP light traps were monthly set in the intradomicile and peridomicile of residences located in the central and beachfront areas of Camaçari, a VL endemic area. The sand fly collection was conducted in two periods: i) period 1—between December 2011 and November 2012 and ii) period 2—August 2014 and July 2015. Sand fly species were identified and detection of L . infantum infection by qPCR was performed in pools of female Lu . longipalpis . For the first time, the parasite load of positive pools was correlated with the number of Lu . longipalpis captured per month in both periods. Correlation analyses between the monthly fluctuation of the sand fly population and bioclimatic indices of the municipality in both collection periods were also performed. In both evaluated periods, more than 98% of the collected sand flies were Lu . longipalpis , confirming the predominance of this species in the region. It was captured mostly in the beachfront area in all months evaluated (99%). For the period 1, Leishmania DNA was detected in 81% of tested pools representing a minimal infection rate of 9.6%. In the period 2, 40% of the pools were positive with a minimal infection rate of 10.2%. Infected sand flies were only detected in the beachfront area in both periods. The parasite load was low and did not vary in the evaluated months despite the number of collected sand flies. No correlation was observed for climatic factors in both areas of Camaçari. These findings emphasize the high risk of Leishmania transmission in Camaçari regardless of the season and that other factors, aside from bioclimatic elements, are influencing the sand fly population monthly fluctuation.

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Natural infection of Lutzomyia trinidadensis (Diptera: Psychodidae) with Leishmania in Barquisimeto, Venezuela

Cited by 11 publications

References 0 publications

Complex ecological interactions across a focus of cutaneous leishmaniasis in Eastern Colombia: novel description of Leishmania species, hosts and phlebotomine fauna

Complex ecological interactions across a focus of cutaneous leishmaniasis in Eastern Colombia: novel description of Leishmania species, hosts and phlebotomine fauna

Natural Leishmania Infection of Lutzomyia auraensis in Madre de Dios, Peru, Detected by a Fluorescence Resonance Energy Transfer–Based Real-Time Polymerase Chain Reaction

Natural infection by Leishmania infantum in the Lutzomyia longipalpis population of an endemic coastal area to visceral leishmaniasis in Brazil is not associated with bioclimatic factors

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