Natural Infection of Alstroemeria caryophyllea with Ornithogalum mosaic virus

Abstract

During a survey for a European Union-funded project on the viruses of Alstroemeria, an A. caryophyllea plant was found expressing virus-like symptoms, including dark green vein banding, necrotic spots, and flower color breaking. In enzyme-linked immunosorbent assays (ELISA), no positive reaction was obtained with antisera to Alstroemeria mosaic, Alstroemeria carla, Cucumber mosaic, Freesia mosaic, or Tobacco rattle virus. A positive ELISA reaction was obtained with potyvirus-specific monoclonal antibodies (Agdia, Elkhart, IN) and antiserum to Ornithogalum mosaic virus (OrMV) (1). In electron microscopy leaf dip preparations of A. caryophyllea, potyvirus-like particles were observed. Using sapinoculation, the virus was transferred to Chenopodium amaranticolor and C. quinoa, resulting in local lesions 6 days postinoculation. The presence of OrMV in both Chenopodium spp. was confirmed by electron microscopy and ELISA with antiserum to OrMV. Sequence alignment of DNA fragments (740 bp) obtained in immunocapture-reverse transcription-polymerase chain reaction on RNA isolated from the suspect virus, using a potyvirus-specific primer set (2), showed 91% homology with the corresponding region of OrMV RNA (GenBank accession no. D00615). The results confirm the infection of A. caryophyllea by OrMV. This is the first report of natural infection of Alstroemeria by OrMV. References: (1) J. T. Burger and M. B. von Wechmar. Phytopathology 79:385, 1989. (2) R. A. A. van der Vlugt et al. Phytopathology 89:148, 1999.

Genetic diversity and molecular evolution ofOrnithogalum mosaic virusbased on the coat protein gene sequence

“…OrMV was first detected in the United States in 1940 ( Smith & Brierley, 1944 ). After that, OrMV has been reported in Netherlands ( Bouwen & Von der Vlugt, 1989 ), France ( Grisoni et al, 2006 ), South Africa ( Burger & Von Wechmar, 1989 ), Israel ( Zeidan et al, 1998 ), India ( Kaur et al, 2011 ), South Korea ( Cho et al, 2016 ), Japan ( Fuji et al, 2003 ), New Zealand ( Wei, Pearson & Cohen, 2006 ), Australia ( Wylie et al, 2013 ) and China ( Chen et al, 2009 ).…”

Alstroemeria yellow spot virus (AYSV): a new orthotospovirus species within a growing Eurasian clade

“…However, viral infections place a large constraint on the production levels and limit global trading [30–32]. To date 18 viruses have been reported from alstroemeria plants (Table S1) [29, 32–42] and among those are isolates from five different orthotospovirus species i.e. , TSWV, impatiens necrotic spot virus (INSV), iris yellow spot virus (IYSV) [32], tomato yellow ring virus (TYRV) [35] and alstroemeria necrotic streak virus (ANSV) [34].…”

Peer Review #1 of "Genetic diversity and molecular evolution of Ornithogalum mosaic virus based on the coat protein gene sequence (v0.2)"

“…OrMV was first detected in the United States in 1940 (Smith & Brierley 1944). After that, OrMV has been reported in Netherlands (Bouwen & von der Vlugt 1989), France (Grisoni et al 2006), South Africa (Burger & von Wechmar 1989), Israel (Zeidan et al 1998), India (Kaur et al 2011), South Korea (Cho et al 2016), Japan (Fuji et al 2003), Zealand (Wei et al 2006), Australia (Wylie et al 2013) and China (Chen et al 2009).…”