Natural Incidence of Sesamia inferens Walker, in Sugarcane Germplasm

Mahesh, P.; Chandran, K.; Srikanth, J.; Nisha, M.; Manjunatha, T.

doi:10.1007/s12355-013-0212-2

Cited by 12 publications

(7 citation statements)

References 2 publications

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“…In addition, based on our investigation, the pest now occurs in more northern regions of China (unpublished data). Many studies of S. inferens have focused on biological characteristics [ 19 – 23 ]. However, the underlying mechanisms that explain the outbreak and distribution of S. inferens remain obscure.…”

Section: Introductionmentioning

confidence: 99%

Exploring Valid Reference Genes for Quantitative Real-Time PCR Analysis in Sesamia inferens (Lepidoptera: Noctuidae)

Sun

Tang

et al. 2015

PLoS ONE

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The pink stem borer, Sesamia inferens, which is endemic in China and other parts of Asia, is a major pest of rice and causes significant yield loss in this host plant. Very few studies have addressed gene expression in S. inferens. Quantitative real-time PCR (qRT-PCR) is currently the most accurate and sensitive method for gene expression analysis. In qRT-PCR, data are normalized using reference genes, which help control for internal differences and reduce error between samples. In this study, seven candidate reference genes, 18S ribosomal RNA (18S rRNA), elongation factor 1 (EF1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ribosomal protein S13 (RPS13), ribosomal protein S20 (RPS20), tubulin (TUB), and β-actin (ACTB) were evaluated for their suitability in normalizing gene expression under different experimental conditions. The results indicated that three genes (RPS13, RPS20, and EF1) were optimal for normalizing gene expression in different insect tissues (head, epidermis, fat body, foregut, midgut, hindgut, Malpighian tubules, haemocytes, and salivary glands). 18S rRNA, EF1, and GAPDH were best for normalizing expression with respect to developmental stages and sex (egg masses; first, second, third, fourth, fifth, and sixth instar larvae; male and female pupae; and one-day-old male and female adults). 18S rRNA, RPS20, and TUB were optimal for fifth instars exposed to different temperatures (−8, −6, −4, −2, 0, and 27°C). To validate this recommendation, the expression profile of a target gene heat shock protein 83 gene (hsp83) was investigated, and results showed the selection was necessary and effective. In conclusion, this study describes reference gene sets that can be used to accurately measure gene expression in S. inferens.

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Section: Introductionmentioning

confidence: 99%

Exploring Valid Reference Genes for Quantitative Real-Time PCR Analysis in Sesamia inferens (Lepidoptera: Noctuidae)

Sun

Tang

et al. 2015

PLoS ONE

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“…According to our previous surveys, this pest now occurs in the more northern regions of China. Many studies of S. inferens have focused on biological characteristics [ 23 , 24 , 25 , 26 , 27 , 28 ]. We previously demonstrated that S. inferens still survived during exposure to low temperatures [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Expression of Genes Encoding Three Small Heat Shock Proteins in Sesamia inferens (Lepidoptera: Noctuidae)

Sun

Tang

et al. 2014

IJMS

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The pink stem borer, Sesamia inferens (Walker), is a major pest of rice and is endemic in China and other parts of Asia. Small heat shock proteins (sHSPs) encompass a diverse, widespread class of stress proteins that have not been characterized in S. inferens. In the present study, we isolated and characterized three S. inferens genes that encode members of the α-crystallin/sHSP family, namely, Sihsp21.4, Sihsp20.6, and Sihsp19.6. The three cDNAs encoded proteins of 187, 183 and 174 amino acids with calculated molecular weights of 21.4, 20.6 and 19.6 kDa, respectively. The deduced amino acid sequences of the three genes showed strong similarity to sHSPs identified in other lepidopteran insects. Sihsp21.4 contained an intron, but Sihsp20.6 and Sihsp19.6 lacked introns. Real-time quantitative PCR analyses revealed that Sihsp21.4 was most strongly expressed in S. inferens heads; Whereas expression of Sihsp20.6 and Sihsp19.6 was highest in eggs. The three S. inferens sHSP genes were up-regulated during low temperature stress. In summary, our results show that S. inferens sHSP genes have distinct regulatory roles in the physiology of S. inferens.

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“…Besides, Saccharum spp. also serve as sources of resistance against pests and accessions resistant to some emerging pests have been identified amongst those maintained at the research centre (Mahesh et al, 2013(Mahesh et al, , 2014(Mahesh et al, , 2015a.…”

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confidence: 99%

RESISTANCE OFSACCHARUMSPP. AGAINSTCHILO SACCHARIPHAGUS INDICUS(KAPUR) (LEPIDOPTERA: CRAMBIDAE) IN INDIA

et al. 2016

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SUMMARYAccessions of four Saccharum spp. from the world collection of sugarcane germplasm maintained at the ICAR-Sugarcane Breeding Institute Research Center, Kannur, Kerala State, India, were screened against Chilo sacchariphagus indicus (Kapur) (Lepidoptera: Crambidae), commonly known as internode borer. Observations on the progression of borer attack in the most susceptible Saccharum officinarum indicated that the incidence began in the first fortnight of July, increased in the next 2 months and reached its peak in September. Thereafter, the incidence decreased in the next 2 months but reached its overall peak in December. Simple correlation coefficients between mean monthly weather parameters and borer incidence were not significant. Percent of canes attacked was significantly lower in 2011 than in 2012 for S. officinarum, Saccharum barberi and Saccharum sinense but did not differ between the years for Saccharum robustum. Amongst the four Saccharum spp., S. robustum showed the lowest borer incidence whereas S. officinarum recorded the lowest attack intensity. Whilst infestation index showed the same trend as percent canes attacked, percent deadhearts did not differ amongst the four Saccharum spp. Considering the 171 accessions evaluated, 29 (16.9%) accessions were resistant, 39 (22.8%) moderately resistant and 103 (60.2%) susceptible to internode borer. Out of the 39 accessions of S. officinarum, none occupied the resistant category whereas 17.9% were in the moderately resistant category. In S. robustum, whilst nearly half (44.5%) the accessions emerged as resistant, a considerable number occupied the moderately resistant category. In S. barberi, no accession was resistant to the borer. In S. sinense, only one accession each represented resistant and moderately resistant categories. Plant morphological characters, yield and quality parameters did not show clear-cut relationship with the three infestation parameters.

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Natural Incidence of Sesamia inferens Walker, in Sugarcane Germplasm

Cited by 12 publications

References 2 publications

Exploring Valid Reference Genes for Quantitative Real-Time PCR Analysis in Sesamia inferens (Lepidoptera: Noctuidae)

Exploring Valid Reference Genes for Quantitative Real-Time PCR Analysis in Sesamia inferens (Lepidoptera: Noctuidae)

Characterization and Expression of Genes Encoding Three Small Heat Shock Proteins in Sesamia inferens (Lepidoptera: Noctuidae)

RESISTANCE OFSACCHARUMSPP. AGAINSTCHILO SACCHARIPHAGUS INDICUS(KAPUR) (LEPIDOPTERA: CRAMBIDAE) IN INDIA

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