Natural incidence of endophytic bacteria in pea cultivars under field conditions

Elvira‐Recuenco, Margarita; Vuurde, J.W.L. van

doi:10.1139/cjm-46-11-1036

Cited by 46 publications

(48 citation statements)

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“…6). This distribution pattern, in which plant sections closer to the ground harbor more bacterial strains, confirmed and extended the findings previously reported for corn (15), pea (14), soybean (28), and sugarcane (31). Over 93% of the degraders isolated from sugarcane clustered within the SBE group.…”

Section: Discussionsupporting

confidence: 90%

Insecticide-Degrading <i>Burkholderia</i> Symbionts of the Stinkbug Naturally Occupy Various Environments of Sugarcane Fields in a Southeast Island of Japan

et al. 2015

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The stinkbug Cavelerius saccharivorus, which harbors Burkholderia species capable of degrading the organophosphorus insecticide, fenitrothion, has been identified on a Japanese island in farmers’ sugarcane fields that have been exposed to fenitrothion. A clearer understanding of the ecology of the symbiotic fenitrothion degraders of Burkholderia species in a free-living environment is vital for advancing our knowledge on the establishment of degrader-stinkbug symbiosis. In the present study, we analyzed the composition and abundance of degraders in sugarcane fields on the island. Degraders were recovered from field samples without an enrichment culture procedure. Degrader densities in the furrow soil in fields varied due to differences in insecticide treatment histories. Over 99% of the 659 isolated degraders belonged to the genus Burkholderia. The strains related to the stinkbug symbiotic group predominated among the degraders, indicating a selection for this group in response to fenitrothion. Degraders were also isolated from sugarcane stems, leaves, and rhizosphere in fields that were continuously exposed to fenitrothion. Their density was lower in the plant sections than in the rhizosphere. A phylogenetic analysis of 16S rRNA gene sequences demonstrated that most of the degraders from the plants and rhizosphere clustered with the stinkbug symbiotic group, and some were identical to the midgut symbionts of C. saccharivorus collected from the same field. Our results confirmed that plants and the rhizosphere constituted environmental reservoirs for stinkbug symbiotic degraders. To the best of our knowledge, this is the first study to investigate the composition and abundance of the symbiotic fenitrothion degraders of Burkholderia species in farmers’ fields.

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Section: Discussionsupporting

confidence: 90%

Insecticide-Degrading <i>Burkholderia</i> Symbionts of the Stinkbug Naturally Occupy Various Environments of Sugarcane Fields in a Southeast Island of Japan

et al. 2015

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“…Therefore we propose that the increased nodulation as a result of better root growth, nodule occupancy by the rhizobial strain and subsequent nitrogen fixation resulted in the increased plant growth parameters. Auxins have been shown to participate in the fundamental responses of nodule morphogenesis, biosynthesis of nodulation factors and induction of root cortical cell mitosis, early nodulin genes and/or nodule-like structures (Srinivasan et al 1996;Dubeikovesky et al 1993;Hirsch et al 1989). The B. thuringiensis-KR1 strain used in this study is also known to produce significant quantities of IAA under in vitro conditions (Selvakumar et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…The application of PGPB has been shown to increase the rate of seedling emergence and plant growth . The mechanisms of growth promotion by PGPB include increased mobilization of insoluble nutrients and subsequent enhanced plant uptake (Lifshitz et al 1987), increased iron availability in the rhizosphere by producing siderophores (Kloepper et al 1980), production of plant growth regulators (Dubeikovesky et al 1993) and increased photosynthetic rates .…”

Section: Introductionmentioning

confidence: 99%

“…In the recent past there has been a renewed interest in the endophytic bacteria of legumes. Studies on pea and soybean plants have demonstrated that the endophytic bacteria induce resistance in pea plants against pathogenic fungi (Castejon-Munoz and Oyarzun 1995;Elvira-Recuenco and van Vuurde 2000) and improve the growth of soybean (Kuklinsky-Sobral et al 2004). Based on the available literature it seems that Enterobacter spp., Pseudomonas spp., and Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

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Coinoculation of Bacillus thuringeinsis-KR1 with Rhizobium leguminosarum enhances plant growth and nodulation of pea (Pisum sativum L.) and lentil (Lens culinaris L.)

Mishra

Selvakumar

et al. 2009

World J Microbiol Biotechnol

102

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Nodulation and the subsequent nitrogen fixation are important factors that determine the productivity of legumes. The beneficial effects of nodulation can be enhanced when rhizobial inoculation is combined with plant-growth-promoting bacteria (PGPB). The PGPB strain Bacillus thuringiensis-KR1, originally isolated from the nodules of Kudzu vine (Pueraria thunbergiana), was found to promote plant growth of field pea (Pisum sativum L.) and lentil (Lens culinaris L.) under Jensen's tube, growth pouch and non-sterile soil, respectively, when co-inoculated with Rhizobium leguminosarum-PR1. Coinoculation with B. thuringiensis-KR1 (at a cell density of 10 6 c.f.u. ml -1 ) provided the highest and most consistent increase in nodule number, shoot weight, root weight, and total biomass, over rhizobial inoculation alone. The enhancement in nodulation due to coinoculation was 84.6 and 73.3% in pea and lentil respectively compared to R. leguminosarum-PR1 treatment alone. The shoot dry-weight gains on coinoculation with variable cell populations of B. thuringiensis-KR1 varied from 1.04 to 1.15 times and 1.03 to 1.06 times in pea and lentil respectively, while root dry weight ratios of coinoculated treatments varied from 0.98 to 1.14 times and 1.08 to 1.33 times in pea and lentil respectively, those of R. leguminosarum-PR1 inoculated treatment at 42 days of plant growth. While cell densities higher than 10 6 c.f.u. ml -1 had an inhibitory effect on nodulation and plant growth, lower inoculum levels resulted in decreased cell recovery and plant growth performance. The results of this study indicate the potential of harnessing endophytic bacteria of wild legumes for improving the nodulation and growth of cultivated legumes.

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“…Garbeva et al [52] reported a concentration of endophytic bacteria in potato seedlings of 4 to 6 log CFU g −1 of vegetable tissue. Elvira-Recuenco and van Vuurde [53] found values of 4 to 8 log CFU g −1 of vegetable tissue in pear cultivars and Zuno et al [24] reported a population of 5.28 log CFU g −1 for Pseudomonas sp in potato seedlings Alpha variety.…”

Section: Resultsmentioning

confidence: 99%

Effect of Acinetobacter sp on Metalaxyl Degradation and Metabolite Profile of Potato Seedlings (Solanum tuberosum L.) Alpha Variety

et al. 2012

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One of the most serious diseases in potato cultivars is caused by the pathogen Phytophthora infestans, which affects leaves, stems and tubers. Metalaxyl is a fungicide that protects potato plants from Phytophthora infestans. In Mexico, farmers apply metalaxyl 35 times during the cycle of potato production and the last application is typically 15 days before harvest. There are no records related to the presence of metalaxyl in potato tubers in Mexico. In the present study, we evaluated the effect of Acinetobacter sp on metalaxyl degradation in potato seedlings. The effect of bacteria and metalaxyl on the growth of potato seedlings was also evaluated. A metabolite profile analysis was conducted to determine potential molecular biomarkers produced by potato seedlings in the presence of Acinetobacter sp and metalaxyl. Metalaxyl did not affect the growth of potato seedlings. However, Acinetobacter sp strongly affected the growth of inoculated seedlings, as confirmed by plant length and plant fresh weights which were lower in inoculated potato seedlings (40% and 27%, respectively) compared to the controls. Acinetobacter sp also affected root formation. Inoculated potato seedlings showed a decrease in root formation compared to the controls. LC-MS/MS analysis of metalaxyl residues in potato seedlings suggests that Acinetobacter sp did not degrade metalaxyl. GC–TOF–MS platform was used in metabolic profiling studies. Statistical data analysis and metabolic pathway analysis allowed suggesting the alteration of metabolic pathways by both Acinetobacter sp infection and metalaxyl treatment. Several hundred metabolites were detected, 137 metabolites were identified and 15 metabolic markers were suggested based on statistical change significance found with PLS-DA analysis. These results are important for better understanding the interactions of putative endophytic bacteria and pesticides on plants and their possible effects on plant metabolism.

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Natural incidence of endophytic bacteria in pea cultivars under field conditions

Cited by 46 publications

References 0 publications

Insecticide-Degrading <i>Burkholderia</i> Symbionts of the Stinkbug Naturally Occupy Various Environments of Sugarcane Fields in a Southeast Island of Japan

Insecticide-Degrading <i>Burkholderia</i> Symbionts of the Stinkbug Naturally Occupy Various Environments of Sugarcane Fields in a Southeast Island of Japan

Coinoculation of Bacillus thuringeinsis-KR1 with Rhizobium leguminosarum enhances plant growth and nodulation of pea (Pisum sativum L.) and lentil (Lens culinaris L.)

Effect of Acinetobacter sp on Metalaxyl Degradation and Metabolite Profile of Potato Seedlings (Solanum tuberosum L.) Alpha Variety

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