Natural Hybrid Zone of Aconitum Species in the Western Carpathians: Linnaean Taxonomy and ISSR Fingerprinting

Sutkowska, Agnieszka; Boroń, P; Mitka, Józef

doi:10.2478/abcsb-2013-00015

Cited by 17 publications

(27 citation statements)

References 52 publications

(40 reference statements)

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“…in Salix (Hardig et al, 2000) and Tilia (Fromm, 2003). Such hybrid complexes are also found in Aconitum (Sutkowska et al, 2013) and, using Linnaean taxonomy, are given the status of nothotaxa (Mitka, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Additivity of ISSR Markers in Natural Hybrids of Related Forest Species Bromus benekenii and B. ramosus (Poaceae)

Sutkowska¹,

Pasierbzziński²,

Bąba³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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The co-occurrence of hybrids and parental species in similar ecological niches poses a question on the role of traits additivity and overdispersion (emergence of new traits) in microevolutionary processes. We analysed genetic polymorphism of Bromus benekenii, B. ramosus and the spontaneous hybrid B. benekenii × B. ramosus in sympatric and allopatric parts of the species distribution in Europe, based on non-coding regions of the taxon genomes (ISSR genetic fingerprinting). We tested 68 individuals in 7 populations, including a hybrid population in N France. Altogether 233 polymorphic ISSR bands (loci) were obtained. We found that the parent species were genetically distinct and the hybrids had an additive pattern of ISSR bands found in the putative parental species (NMDS, STRUCTURE); however, there was evidence of introgression towards B. ramosus (NEWHYBRIDS, UPGMA classifications, Nei's D genetic distance). Bromus benekenii had 72, B. ramosus 21 and the hybrids 9 private bands (genetic overdispersion), probably resulting from the rearranged genomes. Based on its low genetic divergence index DW, the hybrid population seems to be at a young age. We argue that in the face of anthropogenic landscape transformations favouring secondary contacts, the hybrids may competitively replace the parental species in sympatric areas. K Ke ey y w wo or rd ds s: : Genetic additivity, hybrid zone, introgression, ISSR, Linnaean taxonomy, microevolution.

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Section: Introductionmentioning

confidence: 99%

“…We recognized a putative hybrid zone B. ramosus and B. benekenii in northern France during our previous studies (Sutkowska et al, 2013). It is formed by two polyploid species of hybridogenous origin: allotetraploid Bromus benekenii and allohexaploid B. ramosus.…”

Section: Introductionmentioning

confidence: 99%

Additivity of ISSR Markers in Natural Hybrids of Related Forest Species Bromus benekenii and B. ramosus (Poaceae)

Sutkowska¹,

Pasierbzziński²,

Bąba³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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“…This cryptic introgression, i.e. the genetic change not accompanied by any morphological-taxonomic traits, could be a sign of secondary contact influencing the pattern [3,12]. Aconitum plicatum is genetically richer and have the greatest number of unique AFLP bands species.…”

Section: Gene Pool Of Aconitum Plicatum In the Carpathian Taxamentioning

confidence: 99%

“…In Aconitum the cryptic introgression, i.e. the admixture of alien genes not accompanied by the morphological changes, was to be found [12]. The hypothesis on gene flow between the Sudetic and Carpathian Aconitum is assessed by testing relationships between the genetic and taxonomic structure of the populations.…”

Section: Introductionmentioning

confidence: 99%

AFLP analysis reveals infraspecific phylogenetic relationships and population genetic structure of two species of Aconitum in Central Europe

et al. 2015

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The genetic diversity of two Aconitum species endemic to the Carpathian Mountains and Sudetes was studied. A reticulate evolution between them was earlier postulated as an effect of secondary contact. The genetic diversity at the individual and taxonomic levels was examined across the entire geographical ranges of the taxa in 11 populations based on 247 AFLP markers found in 112 individuals in the Sudetes and Western Carpathians. The overall genetic differentiation was greater within the Sudetic A. plicatum (F ST = 0.139, P < 0.001) than within the Carpathian A. firmum (F ST = 0.062, P < 0.001), presumably due to the long-lasting geographic isolation between the Giant Mts and Praděd (Sudetes) populations of the species. Interestingly, relatively distant and presently isolated populations of A. plicatum and A. f. subsp. maninense share a part of their genomes. It could be an effect of their common evolutionary history, including past and present reticulations. The introgression among infraspecific taxa of Aconitum is common, probably as a result of seed dispersal within a distance of ca. 20 km (Mantel's r = 0.36, P = 0.01). Aconitum f. subsp. maninense had the highest genetic diversity indices: Nei's h and rarefied FAr, and divergence index DW (P ≤ 0.05), pointing to its presumably ancient age and long-term isolation.

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“…The ISSR was also successfully employed to assess hybridization and detect hybrid taxa (e.g. Wolfe et al, 1998;Conte et al, 2007;Goldman, 2008;Kramina et al, 2012;Sutkowska et al, 2013). Many studies have demonstrated the efficacy of those markers, both in the phylogenetic analyses and taxonomic relationships of Allium species (Hao et al, 2002;Son et al, 2012) as well as in population and cultivar genetics studies (Hur et al, 2006;Jabbes et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Intraspecific Molecular Variation of Allium ursinum (Amaryllidaceae) Across the Border of Two Subspecies Distribution Ranges

Rola

Lenart‐Boroń

Boroń

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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The study investigates the genetic differentiation among two subspecies of Allium ursinum L., namely A. ursinum subsp. ursinum and subsp. ucrainicum as well as their putative hybrid that is represented by individuals with intermediate morphology. Inter-Simple Sequence Repeats (ISSR) were applied to determine the status of intermediate morphotypes in terms of their genetic pattern and to assess the level of genetic variability within and between various populations of A. ursinum. The study comprises 144 specimens from nine populations along the east-west transect in Poland, which includes localities of both subspecies and their putative hybrid. Among the examined populations, 48 bands were amplified, of which 45 were found to be polymorphic. The principal coordinate analysis (PCoA), the neighbour-net analysis and Mantel test showed a strong correlation between genetic variability and geographic distance. Analysis of molecular variance (AMOVA) revealed that a greater proportion of total genetic variation resided within populations rather than among them. The Structure Bayesian clustering analysis revealed the presence of three distinct genetic groups within studied populations, where 'eastern' genotypes correspond to A. ursinum subsp. ucrainicum, and 'western' to subsp. ursinum; whereas the third genetic group has the largest share in the individuals occurring at the border of the distribution ranges of both subspecies. The emergence of the third genetic group is probably an effect of hybridization events occurring within the secondary contact zone. Typical morphologically intermediate populations occur only in a relatively narrow geographical zone, but the hybrid zone revealed by molecular markers is actually much wider than it is suggested by the morphological pattern of individuals. The current distribution pattern of both subspecies of A. ursinum and their hybrid zone is related to the two main directions of postglacial migration of Fagus sylvatica to the area of Poland. The hybrid zone arose as an effect of the secondary contact of two divergent lineages of A. ursinum.K Ke ey y w wo or rd ds s: : Distribution pattern, genetic variability, hybrid zone, intermediate morphotype, ISSR.

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Natural Hybrid Zone of Aconitum Species in the Western Carpathians: Linnaean Taxonomy and ISSR Fingerprinting

Cited by 17 publications

References 52 publications

Additivity of ISSR Markers in Natural Hybrids of Related Forest Species Bromus benekenii and B. ramosus (Poaceae)

Additivity of ISSR Markers in Natural Hybrids of Related Forest Species Bromus benekenii and B. ramosus (Poaceae)

AFLP analysis reveals infraspecific phylogenetic relationships and population genetic structure of two species of Aconitum in Central Europe

Intraspecific Molecular Variation of Allium ursinum (Amaryllidaceae) Across the Border of Two Subspecies Distribution Ranges

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