Natural history of experimental intestinal atresia: Morphologic and ultrastructural study

Bagłaj, S. M.; Czernik, Jerzy; Kuryszko, Jan; Kuropka, Piotr

doi:10.1053/jpsu.2001.26392

Cited by 16 publications

(18 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Bar =200 mm smooth muscle cells in the submucosa (Masumoto et al 2003). In a morphologic study, Baglaj et al (2001) showed a mild or moderate hypertrophy of the circular muscular layer in the proximal bowel of experimental E19 chick embryos. Hence we presume that the structural changes in the inner circular muscular layer and the abnormal development of VIP immunoreactivity in the DMP are interrelated.…”

Section: Later Ages (E17-e19)mentioning

confidence: 97%

“…We used an experimental chick model (Baglaj et al 2001) that reproduces the most common type of intestinal atresia found in newborn babies (type II intestinal atresia, Grosfeld et al 1979) by transforming a jejuno-ileal segment into a fibrotic cord that separates two blind ends proximal and distal to the atresic stump. We studied normal embryos aged from day 12 (E12) to E20 and experimental embryos aged from E15 to E20.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nervous system development in normal and atresic chick embryo intestine: an immunohistochemical study

Salvi

Vaccaro

Bagłaj³

et al. 2004

Anat Embryol

View full text Add to dashboard Cite

Intestinal motility disorders are a common complication after surgery for neonatal intestinal atresia. Although intestinal atresia causes alterations in the enteric nervous system, especially in its inner structures (nervous fibers in the mucosa, submucous and deep muscular plexuses), how these alterations develop is unclear. The chick model is a useful research tool for investigating the ontogenesis of the enteric nervous system and the pathogenesis of congenital bowel diseases. More information is needed on the overlap between the developing enteric nervous system and intestinal atresia. Because vasoactive intestinal polypeptide and substance P are typical intestinal neuropeptides, and vasoactive intestinal polypeptide acts as a modulator in neurodevelopment and an inhibitor of smooth muscle cell proliferation, our aim in this study was to investigate the distribution of their immunoreactivity in the developing enteric nervous system of normal and experimental chick models. We studied gut specimens excised from normal chick embryos (aged 12-20 days) and experimental chick embryos (aged 15-20 days) that underwent surgical intervention on day 12 to induce intestinal atresia (atresic embryos) or simply to grasp the bowel loop (sham-operated embryos). In normal chick embryos we showed vasoactive intestinal polypeptide and substance P immunoreactivity from day 12 in the submucous and myenteric plexuses. The distribution of peptide immunoreactivity differed markedly in atresic and normal or sham-operated gut embryos. These differences especially affected the inner structures of the enteric nervous system of specimens proximal to atresia and were related to the severity of dilation. Because nerve structures in the gut wall mucosa and submucous and deep muscular plexuses play a role in motility control and stretch sensation in the intestinal wall, our findings in the chick embryo may help to explain how gut motility disorders develop after surgery for neonatal intestinal atresia.

show abstract

Section: Later Ages (E17-e19)mentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Nervous system development in normal and atresic chick embryo intestine: an immunohistochemical study

Salvi

Vaccaro

Bagłaj³

et al. 2004

Anat Embryol

View full text Add to dashboard Cite

show abstract

“…We used an experimental chick model (Baglaj et al , 2001) that reproduces the most common type of intestinal atresia found in newborn babies (type II intestinal atresia) (Grosfeld et al , 1979) by transforming a jejunoileal segment into a fibrotic cord that separates two blind ends proximal and distal to the atresic stump. In this model we have just investigated the development of the enteric nervous system (Parisi Salvi et al , 2004).…”

mentioning

confidence: 99%

Endocrine cells in atresic chick embryo intestine: histochemical and immunohistochemical study

Vaccaro¹,

Salvi²,

Nofroni³

et al. 2009

Eur J Histochem

Self Cite

View full text Add to dashboard Cite

Intestinal motility disorders are an important problem in the postoperative management of patients with intestinal atresia. Intestinal motility could be initiated by luminal factors that activate intrinsic and extrinsic primary afferent nerves involved in the peristaltic reflex. Endocrine cells act as a key point, because they transfer information regarding the intestinal contents and intraluminal pressure to nerve fibers lying in close proximity to the basolateral surface of the epithelium. In chick embryo, experimental intestinal atresia is associated with disorders in the development of the enteric nervous system, related to the severity of intestinal dilation. Our aim was to investigate the distribution pattern of endocrine cells in the developing endocrine system of chick embryo small intestine with experimentally-induced atresia on day 12 and on day 16. Changes in enteroendocrine population were examined in gut specimens (excised proximal and distal to the atresia) from experimental embryos 19 days old and in control sham-operated chick embryos at the same age. Sections from proximal and distal bowel and control bowel were stained with Grimelius silver stain, a valuable histochemical method for detecting the argyrophil and argentophilic cells, and with an immunohistochemical procedure for detecting serotonin and neurotensin immunoreactive cells. In chick embryo proximal bowel, intestinal dilation differed in the various embryos. We found significantly higher enteroendocrine cell counts in proximal bowel than in distal and control bowel. The differences depended on the precociousness of surgery and the severity of dilation. Considering the major contribution of enteroendocrine cells to the peristaltic reflex, our data may help to explain the pathogenesis of motility disorders related to intestinal atresia.

show abstract

“…In fact, atresia has been induced by obstructing the gut blood vessels in experimental trials with ovine fetuses, 22 dogs, 23 rabbits, 24 and chickens. 25,26 In addition, the duration of the vascular occlusion seemed to be a significant factor in chicken. 26 Unfortunately, no data are yet available for calves.…”

Section: Discussionmentioning

confidence: 99%

An unusual colon atresia in a calf: at the junction of the distal loop and transverse colon. A brief overview

Lombardero

Yllera

2014

Organogenesis

View full text Add to dashboard Cite

Congenital defects are those abnormalities present at birth. During embryogenesis, many anomalies can occur. The primitive gut tube lengthens quickly and rotates, allowing the gastrointestinal tract acquire its final position and orientation. Because the colon of large animals is complex, most changes occur in this segment. Thus, in ruminants, colon atresia is the most frequent malformation, affecting mainly ascending colon, at the level of the spiral loop. There are no previous references about a very atypical colon atresia at the junction of distal loop and transverse colon, such we have described in a 5-day-old calf, after a history of abdominal distention and absence of feces at birth, even with a patent anal opening. Atresia coli was detected at distal position of the typical colon atresia, at the junction of distal loop and transverse colon. In addition, the distal blind end was bent into a U-shape supported by the mesocolon. Besides the anatomical findings of this worthwhile atresia coli we discuss its possible etiology, in which local factors, such as a compromised blood supply during embryogenesis, are more consistent than genetic factors. Finding out the causes of atresia coli would help to reduce its incidence, lessen animal suffering and economic loss.

show abstract

Natural history of experimental intestinal atresia: Morphologic and ultrastructural study

Cited by 16 publications

References 12 publications

Nervous system development in normal and atresic chick embryo intestine: an immunohistochemical study

Nervous system development in normal and atresic chick embryo intestine: an immunohistochemical study

Endocrine cells in atresic chick embryo intestine: histochemical and immunohistochemical study

An unusual colon atresia in a calf: at the junction of the distal loop and transverse colon. A brief overview

Contact Info

Product

Resources

About