Natural Gum Reduced/Stabilized Gold Nanoparticles for Drug Delivery Formulations

Dhar, Sheetal; Emani, Maheswarareddy; Shiras, Anjali; Pokharkar, Varsha; Prasad, B. L. V.

doi:10.1002/chem.200801093

Cited by 209 publications

(113 citation statements)

References 76 publications

Supporting

Mentioning

107

Contrasting

Order By: Relevance

“…Recently, the green synthesis or fabrication of Au nanostructures have been considerably studied by using harmless alternative biocompatible molecules such as proteins, peptides, cellulose, soybeans, gellan gum, vitamin B2, starch, and D-glucose as stabilizing or reducing agents [5][6][7][8][9][10][11][12][13][14]. We also developed a facile route for the synthesis of Au nanoparticles by the use of food additives [15].…”

Section: Introductionmentioning

confidence: 99%

Facile green synthesis of gold nanoparticles with gum arabic as a stabilizing agent and reducing agent

Chen

2010

Gold Bull

View full text Add to dashboard Cite

A facile, completely green, and cheap route for the synthesis of Au nanoparticles at 25-75°C has been developed by using only hydrogen tetrachloroaurate as the precursor and gum arabic (GA) simultaneously as a reducing agent and a stabilizing agent. No extra reagents are needed. From the analyses of UV/VIS absorption spectra, TEM, HRTEM, SAED, and XRD patterns, the formation of Au nanoparticles with a fcc structure was recognized. The synthesis reaction was usually finished in 2-4 h. Increasing the reaction temperature increased the formation rate but had no significant effect on the optical property and size of Au nanoparticles. With increasing Au(III) ion concentration or GA concentration, the mean diameter of Au nanoparticles slightly increased. Also, the particle size distribution became broader at higher Au(III) ion concentration or lower GA concentration due to the insufficient protection. Although raising the GA concentration was helpful to reduce Au(III) ions completely and stabilize the Au nanoparticles, too high GA concentration was not suitable for the stabilization of Au nanoparticles because the increased intermolecular force of GA might hinder the dispersion of Au nanoparticles. Furthermore, the resultant Au nanoparticles were found to remain highly stable in the NaCl solution.

show abstract

Section: Introductionmentioning

confidence: 99%

Facile green synthesis of gold nanoparticles with gum arabic as a stabilizing agent and reducing agent

Chen

2010

Gold Bull

View full text Add to dashboard Cite

show abstract

“…Au NPs are readily incorporated by many different types of cells and were found to be suitable in nanomedicine [13] since they show low toxicity [14,15], have convenient characteristics, like the potential to be coated with target molecules [16], and can be used in biomedical imaging [3]. Different in vitro and in vivo studies made use of similar sized Au NPs, which were coated with medical substrates such as drugs or DNA [17][18][19].…”

mentioning

confidence: 99%

Quantification of Gold Nanoparticle Cell Uptake Under Controlled Biological Conditions and Adequate Resolution

et al. 2014

View full text Add to dashboard Cite

Aim:We examined cellular uptake mechanisms of fluorescently labeled polymer-coated gold nanoparticles (NPs) under different biological conditions by two quantitative, microscopic approaches. Materials & methods: Uptake mechanisms were evaluated using endocytotic inhibitors that were tested for specificity and cytotoxicity. Cellular uptake of gold NPs was analyzed either by laser scanning microscopy or transmission electron microscopy, and quantified by means of stereology using cells from the same experiment. Results: Optimal inhibitor conditions were only achieved with chlorpromazine (clathrin-mediated endocytosis) and methyl--cyclodextrin (caveolinmediated endocytosis). A significant methyl--cyclodextrin-mediated inhibition (63-69%) and chlorpromazine-mediated increase (43-98%) of intracellular NPs was demonstrated with both imaging techniques, suggesting a predominant uptake via caveolin-medicated endocytois. Transmission electron microscopy imaging revealed more than 95% of NPs localized in intracellular vesicles and approximately 150-times more NP events/cell were detected than by laser scanning microscopy. Conclusion: We emphasize the importance of studying NP-cell interactions under controlled experimental conditions and at adequate microscopic resolution in combination with stereology. Keywords:In recent years, various types of nanoparticles (NPs; <100 nm in all three dimensions, ISO TS 27687:2008) have been designed for potential biomedical and pharmaceutical applications, such as vehicles for targeted drug delivery, nucleic acids, biomedical imaging or biosensing [1][2][3]. Therefore, an increasing number of research studies have focused on the interaction of NPs with biocellular systems [4,5]. To analyze different types of NP-cell interactions, such as cellular NP uptake, it is essential to have valuable techniques for visualization and quantification, which are mainly based on spectroscopy or microscopy [6].The method of choice for the intracellular detection of NPs depends on the characteristics of the particles (fluorescence, size, and structure or electron density) and on the cellular structure of interest. Advantages of spectroscopy and different microscopic techniques have been reviewed in detail elsewhere [6,7]. In brief, spectroscopy such as inductively coupled plasma-mass spectroscopy [8] allows the quantification and detection of chemical NP elements within the cell, but does not provide any further information on the NP location within the cell. Fluorescence or laser scanning microscopy (LSM) allows real-time analysis or co-localization studies with fluorescently labeled structures [9]. Transmission electron microscopy (TEM) provides the highest resolution and allows quantification of NPs in sub cellular structures [10].

show abstract

“…Improvement in cytotoxicity of EGCG/pNG may be attributed to the greater uptake potential of pNG by endocytosis or enhanced retention permeability, as compared to native EGCG. 22,23 The increase in cytotoxicity of EGCG/pNG may be due to the enrichment in the internalized EGCG conjugate by an endocytosis mechanism, as compared to the passive diffusion mechanism of native EGCG.…”

mentioning

confidence: 99%

The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system

Hsieh

Lu²,

Chen³

et al. 2012

IJN

View full text Add to dashboard Cite

Nanogold particles are commonly used in nanomedicine. We generated physical nanogold (pNG) conjugated with different ratios of epigallocatechin-3-gallate (EGCG) and evaluated its physicochemical properties, antioxidant activity, and cytotoxicity in vitro as well as anticancer activity in vivo. Results showed that the EGCG-pNG conjugates were successfully prepared at ratios between 23:1 and 23:5, with the percentage of EGCG content increasing with the EGCG:pNG ratio from 23:1 (2.0% ± 0.02%) to 23:5 (28% ± 0.3%). EGCG-pNG particles at ratios of 23:1 and 23:5 demonstrated significantly decreased size from 500 to 20 nm and decreasing zeta potentials of 21 mV to -22 mV, respectively. At a ratio of 23:2.5, the EGCGpNG particles (27% EGCG, 50 nm in size, zeta potential of -8 mV) showed longer EGCG activity half-life (110 days vs 5 hours), controlled release (2 hours vs 30 minutes), and higher antioxidant activity (four times), as well as inhibition of tumor cell growth, than controls. The present study indicated that EGCG-pNG possesses promising therapeutic potential, based on its strong free-radical scavenging and anticancer activities.

show abstract

Natural Gum Reduced/Stabilized Gold Nanoparticles for Drug Delivery Formulations

Cited by 209 publications

References 76 publications

Facile green synthesis of gold nanoparticles with gum arabic as a stabilizing agent and reducing agent

Facile green synthesis of gold nanoparticles with gum arabic as a stabilizing agent and reducing agent

Quantification of Gold Nanoparticle Cell Uptake Under Controlled Biological Conditions and Adequate Resolution

The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system

Contact Info

Product

Resources

About