Natural Epizootic Diarrhea of Infant Mice (EDIM) a light and electron microscope study

Rodríguez-Toro, Gerzain

doi:10.1016/0014-4800(80)90058-1

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…There is evidence for this from published studies on rotavirus morphogenesis. Tubular structures have been observed in thin sections of infected cells both in the cytoplasm and in the nuclei and they may be identical to those seen in negatively stained preparations (Banfield et al, 1968;Suzuki & Konno, 1975;Saifet al, 1978;Pearson & McNulty, 1979;Rodriguez-Toro, 1980;Suzuki et al, 1981). It is clear, however, that the complexity of rotavirus morphogenesis (Chasey, 1977;Esparza et al, 1980;Petrie et al, 1981) is reflected also in the variety of related aberrant tubular structures, although the characterization of the protein components of rotatubes is a necessary prerequisite for their formal identification as specific components of the rotavirus capsid.…”

Section: Discussionmentioning

confidence: 92%

Electron Microscopy of Tubular Assemblies Associated with Naturally Occurring Bovine Rotavirus

Chasey¹,

Labram²

1983

Journal of General Virology

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Section: Discussionmentioning

confidence: 92%

Electron Microscopy of Tubular Assemblies Associated with Naturally Occurring Bovine Rotavirus

Chasey¹,

Labram²

1983

Journal of General Virology

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“…Herein we report the gradual evolution of these two pathways during an experimental rotavirus diarrhea in suckling mice. This is one of the most frequently studied animal models, as it displays similarities to infantile gastroenteritis (6)(7)(8) Infi~lion c!f'injunt mice und specimen collection. Litter size was five to seven infant mice.…”

Section: Inserm U290 Paris [Mh J-fd]; Inra-cnrz Laboratoire D'mentioning

confidence: 99%

Intestinal Absorption of Macromolecules during Viral Enteritis: An Experimental Study on Rotavirus-Infected Conventional and Germ-Free Mice

Heyman

Corthier²,

Petit³

et al. 1987

Pediatr Res

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ABSTRACT. Epithelial transport and degradation of horseradish peroxidase (HRP), a macromolecular tracer, was studied in conventional and germ-free suckling mice following an experimental infection with rotavirus. Conventional and germ-free mice developed diarrhea from days 2 to 8 postinfection (pi), with growth failure. In mucosal homogenates, infectious virus detected by immunofluorescence on MA 104 cells was present from day 2 through day 8 pi in germ-free mice, but persisted longer (day 13 pi) in conventional mice. Only mild histological lesions were observed during diarrhea, but obvious macrovacuolation of epithelial cells and increased cellular density occurred during the convalescence period (days 9 to 13 pi). Intact and degraded H R P fluxes from mucosa to serosa were measured in vitro on segments of jejunum mounted in Ussing chambers. Both groups of mice developed increased H R P permeability during the experimental period, but at different times after inoculation: during the diarrheal period (days 2 and 3 pi) conventional mouse epithelium absorbed five times more HRP than noninfected controls and during the convalescence period (days 9 to 13 pi) HRP absorption in germ-free mice rose 10-fold as compared to its level before infection. In both cases, this increase in HRP permeability was entirely due to an increase in intact One of the major problems in the management of infant viral enteritis is to find a suitable diet during and after diarrhea. Of particular interest is the question of whether or not intact foreign proteins are overabsorbed during viral enteritis. Epizootic diarrhea of infant mice provides an interesting model for studying the mechanisms by which the epithelial bamer to macromolecules might be altered in viral enteritis. Intestinal permeability to macromolecular marker HRP was previously studied in isolated jejunal epithelium in different physiological and pathological situations (1-3) including coronavirus infection in piglets (4). Our earlier results indicate that HRP is transported through the enterocytes via two functional pathways: a minor pathway allowing intact protein transport and a major pathway including lvsosomal degradation. In mice. these two Dathwavs do not develop in aUparallel manner d;ring the neonatal ieriod (5). Herein we report the gradual evolution of these two pathways during an experimental rotavirus diarrhea in suckling mice. This is one of the most frequently studied animal models, as it displays similarities to infantile gastroenteritis (6-8). Our results indicate that rotavirus infection in mice causes an increase in intact HRP intestinal permeability which is dependent on intestinal microflora. METHODSHRP absorption, probably via a transcellular route, and dAnimols, Germ-free and conventional C3H/HeJ supplied occurred without any in degraded HRP trans-by Centre de Sklection et d'Elevage des Animaux de Laboratoire, port. These results indicate that in mice, rotavirus infection Orlkans-la Source, France, were used for experimentation. Germcauses a transient ris...

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“…In most reports about viral infections of the intestine, investigators have emphasized cytopathic changes in the epithelial cells, which result in diarrhea (adenoviruses: Yunis et al, 1975;rotaviruses: Gray et al, 1973;Holmes et al, 197.5;Suzuki and Komo, 1975;Snodgrass et al, 1977;Pearson and McNulty, 1978;Rodriguez-Toro, 1980;and coronavirus: Doughri et al, 1976;Takeuchi et al, 1976). In the present study, SLE virus was found to primarily infect the lamina propria of the intestine, where it replicated in blood, endothelial, nerve, and muscle cells, and only rarely involved the intestinal epithelium.…”

Section: Discussionmentioning

confidence: 99%

Visceral target organs in systemic St. Louis encephalitis virus infection of hamsters

Harrison

Murphy

Gardner

1982

Experimental and Molecular Pathology

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Ultrastructural aspects of St. Louis encephalitis virus infection of the major extraneutral organs and tissues of suckling hamsters were examined. In the pancreas, both the exocrine and endocrine portions were equally affected by the virus. A feature apparently unique to flaviviruses was the accumulation of virus particles in all types of secretory granules in this organ. Virus particles were seen within myocardial fibers and within the smooth muscle cells and endothehal cells of small blood vessels of the heart. In the intestines, the lamina propria was the most severely infected, with virus particles accumulated in all cell types.

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Natural Epizootic Diarrhea of Infant Mice (EDIM) a light and electron microscope study

Cited by 8 publications

References 13 publications

Electron Microscopy of Tubular Assemblies Associated with Naturally Occurring Bovine Rotavirus

Electron Microscopy of Tubular Assemblies Associated with Naturally Occurring Bovine Rotavirus

Intestinal Absorption of Macromolecules during Viral Enteritis: An Experimental Study on Rotavirus-Infected Conventional and Germ-Free Mice

Visceral target organs in systemic St. Louis encephalitis virus infection of hamsters

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