Natural Duck Hepatitis B Virus Infection in Australia

Js, Freiman; Cossart, Yvonne E.

doi:10.1038/icb.1986.51

Cited by 26 publications

(8 citation statements)

References 8 publications

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“…Seventy uninfected 1-day-old Pekin Aylesbury cross-bred ducks were inoculated intraperitoneally with 100 pl of serum containing the recently isolated Australian strain of DHBV (5). The inoculum contained 8 X lo9 genome equivalents per ml.…”

Section: Methodsmentioning

confidence: 99%

Experimental duck hepatitis B virus infection: Pathology and evolution of hepatic and extrahepatic infection

et al. 1988

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Seventy, 1-day-old ducklings inoculated intraperitoneally with duck hepatitis B virus and 30 controls have been studied over a 2-year period. Infection with duck hepatitis B virus occurred in all inoculated ducks, although this was not associated with clinical morbidity. Duck hepatitis B virus DNA was first detected in liver on Day 3, in pancreatic acinar cells on Day 4, serum on Day 6, splenic red and white pulp on Day 7 and in the renal glomurulus on Day 14, using a combination of dot, Southern blot and in situ hybridization techniques. Peak levels of circulating virus, as determined by DNA polymerase levels, occurred 1 to 4 weeks postinoculation. Mild degrees of portal inflammation were seen in sections of liver tissue in both infected and control ducks. However, moderately severe inflammatory changes were present in 8 of 22 infected birds compared with 0 of 18 controls (p less than 0.025). Appearance of this inflammatory infiltrate 6 weeks postinoculation coincided with a decrease in levels of duck hepatitis B virus DNA in hepatocytes and within the pancreatic acinar cells. At the same time, duck hepatitis B virus DNA became increasingly localized to the splenic germinal centers, and viral DNA was first detected in pancreatic islet cells. No histological changes accompanied the extra-hepatic tissue infection. The sequence and significance of duck hepatitis B virus infection in liver and extra-hepatic tissues is discussed in relation to the pathogenesis of hepatitis B virus infection in man.

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Section: Methodsmentioning

confidence: 99%

Experimental duck hepatitis B virus infection: Pathology and evolution of hepatic and extrahepatic infection

et al. 1988

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“…Pekin ducks congenitally infected with DHBV and followed for several years in various studies have not developed liver tumours (Freiman & Cossart 1986;Cova et al, 1990;Cullen et al, 1990;Lambert et al, 1991). In fact HCC has been found only in domestic brown ducks from a single area of China, Qidong and only four Chinese duck HCCs had so far been described (Omata et al, 1983;Marion et al, 1984;Yokosuka et al, 1985).…”

mentioning

confidence: 99%

Duck hepatitis B virus infection, aflatoxin B1 and liver cancer in domestic Chinese ducks

Cova

Mehrotra²,

Wild³

et al. 1994

Br J Cancer

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The oncogenicity of Duck hepatitis B virus (DHBV) is unclear since hepatocellular carcinomas (HCCs) have been reported only in domestic ducks in Qidong, an area of China where hepatitis B virus (HBV) and aflatoxin B1 (AFB1) are risk factors for liver cancer in man. In order to better define the association between DHBV infection, AFB1 and HCC we analysed a series of 16 duck liver samples collected from local farms in Qidong. HCC was found in eight and cirrhosis in one of these samples. Furthermore bile duct proliferation, characteristic of AFB1 exposure in ducks and other animal species, was found in these ducks. Integration of DHBV DNA into cellular DNA was observed in only one out of four DHBV positive HCCs, indicating that viral integration is not prerequisite for tumour development. In four remaining HCCs the polymerase chain reaction (PCR) failed to show any DHBV DNA suggesting that liver tumours do occur in polymerase chain reaction (PCR) failed to show any DHBV DNA suggesting that liver tumours do occur in these ducks in the absence of DHBV infection. In addition, AFB1-DNA adducts were detected by hplc-immunoassay in one such DHBV-negative tumour. In summary we demonstrate that risk factors other than DHBV, including AFB1 exposure, may be important in duck liver carcinogenesis in Qidong. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5

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“…The prototype Australian strain of DHBV 15 was used to infect five one-day-old ducklings. These animals were used as the source biohazardous ducks during peak viraemia (from 14 to 21 days of age).…”

Section: Dhbv Positive Source Animalsmentioning

confidence: 99%