Natural dietary agents can protect against DMBA genotoxicity in lymphocytes as revealed by single cell gel electrophoresis assay

De, Sajal; Ganguly, Chaiti; Das, Sukta

doi:10.1002/tcm.10062

Cited by 13 publications

(5 citation statements)

References 23 publications

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“…Approximately 75% of the world’s populations depend on CAM as reported by the world health organization reports ( Grawish et al, 2010 ). Several natural products has been suggested to be beneficial for reducing DMBA toxicity including hops, rosemary, cat's claw ( El Kholy et al, 2013 ) and bitter gourd and tomato ( De et al, 2003 ). Bovine colostrum (BC) has been known and used for years in the treatment of infectious diseases in humans ( Struff and Sprotte, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Ameliorative effects of colostrum against DMBA hepatotoxicity in rats

Abdelmeguid

Khalil

Badr

et al. 2021

Saudi Journal of Biological Sciences

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Colostrum, the sole diet for newborns, is an emerging nutraceutical. To date, the chemopreventive effect of Bovine Colostrum against liver injury induced by the potent carcinogen, 7,12-dimethyl-Benz[a]anthracene (DMBA) is unexplored. Humans are daily exposed to DMBA which is a highly lipophilic environmental organic pollutant. The study aimed to investigate the hepatoprotective role of Bovine Colostrum against DMBA-induced hepatotoxicity using a rat model. Fifty male rats were divided into five groups; GI (control), GII (olive oil, vehicle for DMBA), GIII (DMBA), GIV (DMBA + Bovine Colostrum), GV (Bovine Colostrum). After 12 weeks, body weight changes and mortality were calculated. Histological and ultrastructural examinations of liver tissue were performed. Expressions of p53, TGFβ2, TNF-α, S6K2, and c20orf20 were assessed by RT-PCR. Post-treatment with Bovine Colostrum increased both the body weight and the survival rate of rats treated with DMBA. In addition, remarkable protection against the pathological effect of DMBA was noted. Ultrastructurally, Bovine Colostrum ameliorated/prevented most of the toxic effects of DMBA on hepatocytes, including irregularities of nuclear envelope, clumping, and margination of heterochromatin aggregates, segregated nucleoli, and mitochondrial pleomorphism. Bovine Colostrum administration down-regulated p53, C20orf20, and S6K2 mRNA levels, and up-regulated TNF-α and TGFβ2. In conclusion, Bovine Colostrum have a protective effect against DMBA-induced toxicity on the liver of albino rats. Consequently, Bovine Colostrum may prevent polycyclic aromatic hydrocarbons-induced hepatotoxicity and may be useful in promoting human health if supplemented in the diet.

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Section: Introductionmentioning

confidence: 99%

Ameliorative effects of colostrum against DMBA hepatotoxicity in rats

Abdelmeguid

Khalil

Badr

et al. 2021

Saudi Journal of Biological Sciences

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“…MC pulp and seed powders exhibit stronger anti-oxygenic activity than other solvent extracts, while MC pulp and its extracts show slightly higher anti-oxygenic activity than MC seed and its extracts [107] . De et al have found that fresh juices of MC and tomato could also protect from DMBA-induced DNA damage, but not as effectively as the single agents [108] .…”

Section: Introductionmentioning

confidence: 99%

Bitter melon: a panacea for inflammation and cancer

Dandawate

Subramaniam

Padhyé³

et al. 2016

Chinese Journal of Natural Medicines

114

123

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Nature is a rich source of medicinal plants and their products that are useful for treatment of various diseases and disorders. Momordica charantia, commonly known as bitter melon or bitter gourd, is one of such plants known for its biological activities used in traditional system of medicines. This plant is cultivated in all over the world, including tropical areas of Asia, Amazon, east Africa, and the Caribbean and used as a vegetable as well as folk medicine. All parts of the plant, including the fruit, are commonly consumed and cooked with different vegetables, stir-fried, stuffed or used in small quantities in soups or beans to give a slightly bitter flavor and taste. The plant is reported to possess anti-oxidant, anti-inflammatory, anti-cancer, anti-diabetic, anti-bacterial, anti-obesity, and immunomodulatory activities. The plant extract inhibits cancer cell growth by inducing apoptosis, cell cycle arrest, autophagy and inhibiting cancer stem cells. The plant is rich in bioactive chemical constituents like cucurbitane type triterpenoids, triterpene glycosides, phenolic acids, flavonoids, essential oils, saponins, fatty acids, and proteins. Some of the isolated compounds (Kuguacin J, Karaviloside XI, Kuguaglycoside C, Momordicoside Q–U, Charantin, α-eleostearic acid) and proteins (α-Momorcharin, RNase MC2, MAP30) possess potent biological activity. In the present review, we are summarizing the anti-oxidant, anti-inflammatory, and anti-cancer activities of Momordica charantia along with a short account of important chemical constituents, providing a basis for establishing detail biological activities of the plant and developing novel drug molecules based on the active chemical constituents.

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“…To date there is no evidence in literature that investigate the effect of quercetin on both genomes. A lot of data are available regarding the quercetin effect on nuclear DNA by measuring nonspecific DNA damage by comet assay [8,[14][15][16]18,20,29,32,40,[48][49][50][51][54][55][56]59]. This technique, however, though sensitive, fast and easy, highlights only damages in terms of break of single or double strand of nuclear DNA.…”

Section: Discussionmentioning

confidence: 99%

Effect of quercetin on oxidative nuclear and mitochondrial DNA damage

et al. 2008

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Quercetin is a well-investigated antioxidant known to protect cells against oxidative nuclear DNA damage. There is no knowledge regarding its effect on oxidative mitochondrial DNA damage. In this study we investigated the effect of quercetin on oxidatively-injured DNA. Cell-free and cell studies were performed. Cell-free analyses carried out on plasmidic DNA showed that quercetin protects from all oxidative challenges used. Cellular studies were carried out on NCTC 2544 cells which were insulted with hydrogen peroxide and UVC radiations. Nuclear and mitochondrial DNAs were analysed by measuring DNA damage with a quantitative polymerase chain reaction. Quercetin supplementation showed significant genoprotective activity on mitochondrial DNA when hydroperoxide was used. The evidence of the protection afforded by quercetin suggests that this flavonoid may play an important role on mitochondrial genome stability.

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Natural dietary agents can protect against DMBA genotoxicity in lymphocytes as revealed by single cell gel electrophoresis assay

Cited by 13 publications

References 23 publications

Ameliorative effects of colostrum against DMBA hepatotoxicity in rats

Ameliorative effects of colostrum against DMBA hepatotoxicity in rats

Bitter melon: a panacea for inflammation and cancer

Effect of quercetin on oxidative nuclear and mitochondrial DNA damage

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