Natural deep eutectic systems, an emerging class of cryoprotectant agents

Jesus, Ana Rita; Meneses, Liane; Duarte, Ana Rita C.; Paiva, Alexandre

doi:10.1016/j.cryobiol.2021.05.002

Cited by 37 publications

(44 citation statements)

References 34 publications

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“…These systems are composed by natural metabolites, such as trehalose, glucose, proline and sorbitol. For the majority of the systems, water was required to ensure the formation of the eutectic mixture which was previously confirmed by polarized optical microscopy (POM) and nuclear magnetic resonance (NMR) 26 .…”

Section: Resultsmentioning

confidence: 80%

“…NADES presented in Table 1 were prepared as described previously by our group 26 . These systems are composed by natural metabolites, such as trehalose, glucose, proline and sorbitol.…”

Section: Resultsmentioning

confidence: 99%

“…In our previous report 26 we have prepared a library of NADES composed by natural metabolites, sugars, amino acids, and choline derivatives and showed that they were able to successfully cryopreserve two different types of cells, L929 and HacaT, when used at 10% (w/v). what we propose here is to use NADES at high concentration as CPA for the vitrification of mammalian cells.…”

Section: Introductionmentioning

confidence: 99%

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Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells

Jesus¹,

Duarte²,

Paiva³

2022

Sci Rep

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In this work we present the potential of Natural Deep Eutectic Systems (NADES) as new vitrification media for the cryopreservation of mammalian cells. Several NADES composed of natural metabolites were prepared and tested as CPAs in two cell lines, L929 and HacaT cells. After the harvesting, cells were mixed with the eutectic systems, and frozen directly into liquid nitrogen to achieve a vitreous state. Then, the cells were thawed and it was observed that NADES were able to exert a significant cryoprotective effect in L929 cells, when compared with DMSO or in the absence of a CPA. For HacaT cells, only a eutectic system showed a slightly improvement in cell survival, while DMSO caused complete cell death. Moreover, the thermal behaviour of the best systems was studied for further understanding the protective properties of NADES as CPAs, and have shown a significant difference in terms of Tm and Tc when compared with DMSO and water. Additionally, the results obtained showed that NADES can be maintained in the growth media after the thawing step, without compromising cell viability. In summary, we have shown the great potential of NADES to be used as CPAs for the cryopreservation of different cell types, using the vitrification method.

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Section: Resultsmentioning

confidence: 80%

“…NADES presented in Table 1 were prepared as described previously by our group 26 . These systems are composed by natural metabolites, such as trehalose, glucose, proline and sorbitol.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells

Jesus¹,

Duarte²,

Paiva³

2022

Sci Rep

Self Cite

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“…[6][7][8][9][10] Recently, a contemporary class of green solvents, Deep Eutectic Solvents (DESs), introduced by Abbott et al, [11] are regarded as a potential new type of cryoprotectants. [12,13] DESs are frequently classified as Ionic Liquids' (ILs) analogues. They possess non-toxic character and require one-pot synthesis without byproducts or further purification.…”

Section: Introductionmentioning

confidence: 99%

Time‐Dependent Hydrogen Bond Network Formation in Glycerol‐Based Deep Eutectic Solvents

et al. 2022

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Over the last few years, Deep Eutectic Solvents have gained popularity as a novel class of green solvents, due to their feasible synthesis and overall low production costs. The properties of glycerol (Gly)-based Deep Eutectic Solvents are frequently associated with the formation of an extended hydrogen bond network. In this study, two-dimensional Nuclear Magnetic Resonance (NMR) spectroscopy is employed to analyse the effect of glycerol oversaturation of the hydrogen bond acceptor, choline chloride (ChCl) on the structural arrangement of glyceline (molar ratio 1 : 2 ChCl:Gly), selected to represent Gly-based Deep Eutectic Solvents. The rearrangement of glycerol molecules, additionally trapping water molecules inside of isolated clusters, is revealed during a time-resolved analysis, performed in the presence of various fractions of water added to solvent. 200 % oversaturated Deep Eutectic Solvent (1 : 4 ChCl:Gly) is found to be a suitable cryoprotectant candidate, based on the revealed glycerol-water interactions.

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“…Low-molecular-weight sugars, such as glucose, glycerol, sucrose, trehalose, mannitol, and sorbitol, have been successfully used as cryoprotective agents for LAB during freeze-drying. 16 As CP contains high glucose, fructose, and sucrose levels, it may be suitable as a cryoprotective agent. 17 Therefore, a practical strategy for the utilization of CP in the production of biobased chemicals is required.…”

Section: Introductionmentioning

confidence: 99%

Efficient Citrus (Citrus unshiu) Byproduct Extract-Based Approach forLactobacillus sakeiWiKim31 Shelf-Life Extension

Kim¹,

Jeong²,

Hwang³

et al. 2021

ACS Omega

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Lactic acid bacteria produce various bioactive compounds widely used in human healthcare. However, studies on cryoprotective agents for the efficient storage of lactic acid bacteria after freeze-drying are still lacking. Here, we report the shelf-life extension effects of a highly efficient and eco-friendly cryoprotective agent and a cold adaptation method on Lactobacillus sakei WiKim31. Cold adaptation of L. sakei WiKim31 increased exopolysaccharide expression in response to abiotic stress. As a possible cryoprotective agent, the citrus byproduct (CP) contains a variety of sugars, amino acids, and cations, exhibiting high antioxidant activity. L. sakei WiKim31 powders formulated with CP or a mixture of soy powder (SP) and CP exhibited high cell viability at 58.3 and 76.3%, respectively, after 56 days of storage. These results indicate that CP can be efficiently used as a novel cryoprotective agent either alone or in combination with SP to improve the storage conditions of L. sakei WiKim31 and preserve it longer.

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Natural deep eutectic systems, an emerging class of cryoprotectant agents

Cited by 37 publications

References 34 publications

Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells

Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells

Time‐Dependent Hydrogen Bond Network Formation in Glycerol‐Based Deep Eutectic Solvents

Efficient Citrus (Citrus unshiu) Byproduct Extract-Based Approach forLactobacillus sakeiWiKim31 Shelf-Life Extension

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