Natural deep eutectic solvents as thermostabilizer for Humicola insolens cutinase

Romano, Angela; Varriale, Simona; Pezzella, Cinzia; Totaro, Grazia; Andanson, Jean‐Michel; Verney, Vincent; Sisti, Laura

doi:10.1016/j.nbt.2023.05.006

Cited by 6 publications

(2 citation statements)

References 42 publications

(73 reference statements)

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“…DES has been used for various applications in enzymatic catalysis as a reaction medium, allowing it to carry out reactions with substrates of different polarities [ 63 , 70 , 71 ]. DES in biocatalysis serves both as solvents and substrates.…”

Section: Upstream Bioprocessingmentioning

confidence: 99%

Upstream and Downstream Bioprocessing in Enzyme Technology

Guajardo,

Schrebler

2023

Pharmaceutics

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The development of biotransformation must integrate upstream and downstream processes. Upstream bioprocessing will influence downstream bioprocessing. It is essential to consider this because downstream processes can constitute the highest cost in bioprocessing. This review comprehensively overviews the most critical aspects of upstream and downstream bioprocessing in enzymatic biocatalysis. The main upstream processes discussed are enzyme production, enzyme immobilization methodologies, solvent selection, and statistical optimization methodologies. The main downstream processes reviewed in this work are biocatalyst recovery and product separation and purification. The correct selection and combination of upstream and downstream methodologies will allow the development of a sustainable and highly productive system.

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Section: Upstream Bioprocessingmentioning

confidence: 99%

Upstream and Downstream Bioprocessing in Enzyme Technology

Guajardo,

Schrebler

2023

Pharmaceutics

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“…The results indicated that the enzyme's half-life at 90°C tripled when 20% ChDHC:Xyl (1:1) was utilized [24].…”

Section: Introductionmentioning

confidence: 99%

Deep eutectic solvent as an additive to improve enzymatic hydrolysis of polyethylene terephthalate (PET)

Zheng,

Feng,

et al. 2024

Preprint

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In recent years, the research topic of hydrolysis of polyethylene terephthalate (PET) using bioenzymes has attracted a lot of attention and can be divided into two parts: enzymatic surface modification of polyester fibres and biodegradation of PET waste. The high crystallinity of polyester fibres makes the enzyme surface modification study very challenging compared to PET waste. Deep eutectic solvents (DES) can create a favourable environment for proteins and represent a new generation of biodegradable solvents. However, there are few studies on the use of DES to enhance enzymatic degradation. For these reasons, we attempted to hydrolyse PET with DES-activated enzymes to increase the hydrolysis yield and thus improve PET modification. We investigated the effects of DES type, molar ratio and concentration on enzymatic hydrolysis, utilizing betaine and choline chloride as hydrogen bond acceptors and polyol as a hydrogen bond donor. Humicola insolens cutinase (HiC) is used as the main biocatalyst for PET fabric hydrolysis. The results showed that a low concentration (20% v/v) of deep eutectic solvents (DES) (betaine:sorbitol; 1:2 molar ratio) induced a significant increase in hydrolysis yield (more than 1.5 times). The boosting effect primarily resulted from the benefits of DES itself rather than from the superimposition of individual components. Further studies revealed that HiCexhibited high relative enzyme activity and stability at low concentrations of DES. In addition, spectral analyses showed that DES effectively preserved the structure of HiC. Our work sheds light on the development of efficient and sustainable method to improve HiC hydrolysis of PET fabric, opening up new opportunities and possibilities for comprehensive utilization of DES in bio-modification of PET fabric.

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Deep Eutectic Solvent as an Additive to Improve Enzymatic Hydrolysis of Polyethylene Terephthalate (PET)

Zheng,

Feng,

et al. 2024

J Polym Environ

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Natural deep eutectic solvents as thermostabilizer for Humicola insolens cutinase

Cited by 6 publications

References 42 publications

Upstream and Downstream Bioprocessing in Enzyme Technology

Upstream and Downstream Bioprocessing in Enzyme Technology

Deep eutectic solvent as an additive to improve enzymatic hydrolysis of polyethylene terephthalate (PET)

Deep Eutectic Solvent as an Additive to Improve Enzymatic Hydrolysis of Polyethylene Terephthalate (PET)

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